################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 41 TCCGGA T^CCGGA AcsI 317 AAATTC [GA]^AATT[CT] AcyI 64 GACGCC G[GA]^CG[CT]C AfaI 531 GTAC GT^AC AlwNI 223 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 317 AAATTC [GA]^AATT[CT] AspHI 442 GAGCTC G[AT]GC[AT]^C AvaI 602 CTCGGG C^[CT]CG[GA]G AviII 29 TGCGCA TGC^GCA BanI 85 GGCACC G^G[CT][GA]CC BbsI 544 GAAGAC GAAGAC(2/6) BlpI 439 GCTGAGC GC^T[ACGT]AGC Bpu1102I 439 GCTGAGC GC^T[ACGT]AGC BpuAI 544 GAAGAC GAAGAC(2/6) BsaHI 64 GACGCC G[GA]^CG[CT]C BsaI 127 GAGACC GGTCTC(1/5) BsaJI 197 CCCAGG C^C[ACGT][ACGT]GG BsaWI 41 TCCGGA [AT]^CCGG[AT] BseAI 41 TCCGGA T^CCGGA BsgI 425 GTGCAG GTGCAG(16/14) BsiEI 366 CGACCG CG[GA][CT]^CG BsiHKAI 442 GAGCTC G[AT]GC[AT]^C BsoBI 602 CTCGGG C^[CT]CG[GA]G BspEI 41 TCCGGA T^CCGGA BsrBI 433 GAGCGG CCGCTC(-3/-3) BsrDI 351 CATTGC GCAATG(2/0) BsrI 510 ACTGG ACTGG(1/-1) BssHII 78 GCGCGC G^CGCGC BssSI 109 CACGAG CACGAG(-5/-1) BstF5I 165 CATCC GGATG(2/0) CelII 439 GCTGAGC GC^T[ACGT]AGC DraII 569 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 723 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 569 AGGTCCT [GA]G^G[ACGT]CC[CT] FokI 165 CATCC GGATG(9/13) FspI 29 TGCGCA TGC^GCA HapII 42 CCGG C^CGG Hin1I 64 GACGCC G[GA]^CG[CT]C HpaII 42 CCGG C^CGG MroI 41 TCCGGA T^CCGGA MspI 42 CCGG C^CGG NlaIII 270 CATG CATG^ PpuMI 569 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 169 CAGCTG CAG^CTG RsaI 531 GTAC GT^AC SacI 442 GAGCTC GAGCT^C SbfI 48 CCTGCAGG CCTGCA^GG ScaI 530 AGTACT AGT^ACT SmlI 682 CTTGAG C^T[CT][GA]AG Sse8387I 48 CCTGCAGG CCTGCA^GG TaqI 288 TCGA T^CGA TspRI 509 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 288 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AvaII 105 GGACC G^G[AT]CC 570 GGTCC BanII 290 GAGCCC G[GA]GC[CT]^C 442 GAGCTC BbvCI 70 GCTGAGG CCTCAGC(-5/-2) 120 GCTGAGG BbvI 93 GCAGC GCAGC(8/12) 247 GCAGC BseRI 73 GAGGAG GAGGAG(10/8) 430 GAGGAG BsiYI 390 CCGTAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 723 CCTGAATGAGG BslI 390 CCGTAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 723 CCTGAATGAGG BsmAI 127 GAGAC GTCTC(1/5) 598 GAGAC BssKI 198 CCAGG ^CC[ACGT]GG 417 CCTGG BstNI 198 CCAGG CC^[AT]GG 417 CCTGG BstYI 38 AGATCC [GA]^GATC[CT] 326 AGATCC EcoRII 198 CCAGG ^CC[AT]GG 417 CCTGG HaeIII 387 GGCC GG^CC 720 GGCC HinfI 285 GAGTC G^A[ACGT]TC 600 GACTC MaeII 143 ACGT A^CGT 423 ACGT MflI 38 AGATCC [GA]^GATC[CT] 326 AGATCC MvaI 198 CCAGG CC^[AT]GG 417 CCTGG NlaIV 44 GGAGCC GG[ACGT]^[ACGT]CC 85 GGCACC PleI 600 GACTC GAGTC(4/5) 285 GAGTC PstI 49 CTGCAG CTGCA^G 91 CTGCAG ScrFI 198 CCAGG CC^[ACGT]GG 417 CCTGG SfaNI 361 GATGC GCATC(5/9) 164 GCATC SfcI 49 CTGCAG C^T[GA][CT]AG 91 CTGCAG TseI 93 GCAGC G^C[AT]GC 247 GCAGC Tsp45I 490 GTGAC ^GT[GC]AC 507 GTCAC XhoII 38 AGATCC [GA]^GATC[CT] 326 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AccII AciI AluI AlwI BmyI BpmI Bsp1286I BstUI Cac8I CfoI Cfr13I DdeI DpnII EarI FauI Fnu4HI HgaI HhaI HinP1I ItaI Ksp632I MaeIII MboI MboII MnlI MspA1I MvnI MwoI NdeII Sau3AI Sau96I Tsp509I