Restriction map for pF1KB9359
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     MamI        MfeI        MflI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AocI          762  CCTCAGG              CC^T[ACGT]AGG
ApaI          829  GGGCCC               GGGCC^C
AspHI         670  GAGCTC               G[AT]GC[AT]^C
AvrII         432  CCTAGG               C^CTAGG
BalI          239  TGGCCA               TGG^CCA
BbsI          229  GTCTTC               GAAGAC(2/6)
BfaI          433  CTAG                 C^TAG
BlnI          432  CCTAGG               C^CTAGG
BpuAI         229  GTCTTC               GAAGAC(2/6)
BsaAI         112  TACGTA               [CT]AC^GT[GA]
BsaI          343  GAGACC               GGTCTC(1/5)
BsiHKAI       670  GAGCTC               G[AT]GC[AT]^C
BsrBI         773  CCGCTC               CCGCTC(-3/-3)
BsrFI         497  ACCGGC               [GA]^CCGG[CT]
BstAPI         84  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        762  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        497  ACCGGC               [GA]^CCGG[CT]
EaeI          239  TGGCCA               [CT]^GGCC[GA]
Eco81I        762  CCTCAGG              CC^T[ACGT]AGG
HgaI          272  GCGTC                GACGC(5/10)
KspI           55  CCGCGG               CCGC^GG
MaeI          433  CTAG                 C^TAG
MaeII         113  ACGT                 A^CGT
MluNI         239  TGGCCA               TGG^CCA
MscI          239  TGGCCA               TGG^CCA
MslI          702  CACTGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI         449  GGGACCT              [GA]G^G[AT]CC[CT]
SacI          670  GAGCTC               GAGCT^C
SacII          55  CCGCGG               CCGC^GG
SmlI          442  CTCAAG               C^T[CT][GA]AG
SnaBI         112  TACGTA               TAC^GTA
XcmI          242  CCATGAACTACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          115  GTAC                 GT^AC
              591  GTAC                
AlwI          255  GGATC                GGATC(4/5)
              692  GGATC               
AlwNI          85  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              298  CAGCTCCTG           
Aor51HI       107  AGCGCT               AGC^GCT
              212  AGCGCT              
AvaI           38  CCCGGG               C^[CT]CG[GA]G
              809  CCCGGG              
BanI           22  GGCACC               G^G[CT][GA]CC
              646  GGTGCC              
BsmAI         343  GAGAC                GTCTC(1/5)
              394  GTCTC               
BsoBI          38  CCCGGG               C^[CT]CG[GA]G
              809  CCCGGG              
DraII         449  GGGACCT              [GA]G^G[ACGT]CC[CT]
              828  AGGGCCC             
DrdI           70  GACCAGCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              385  GACCACGCTGTC        
DsaI           55  CCGCGG               C^C[GA][CT]GG
              611  CCACGG              
Eco47III      107  AGCGCT               AGC^GCT
              212  AGCGCT              
EcoO109I      449  GGGACCT              [GA]G^G[ACGT]CC[CT]
              828  AGGGCCC             
MaeIII        515  GTGAC                ^GT[ACGT]AC
              859  GTCAC               
MboII         171  GAAGA                GAAGA(8/7)
              230  TCTTC               
MspA1I         55  CCGCGG               C[AC]G^C[GT]G
              145  CAGCGG              
PstI           82  CTGCAG               CTGCA^G
              629  CTGCAG              
RsaI          115  GTAC                 GT^AC
              591  GTAC                
SmaI           38  CCCGGG               CCC^GGG
              809  CCCGGG              
SphI          317  GCATGC               GCATG^C
              540  GCATGC              
StuI          524  AGGCCT               AGG^CCT
              639  AGGCCT              
TaqI          362  TCGA                 T^CGA
              740  TCGA                
Tsp45I        515  GTGAC                ^GT[GC]AC
              859  GTCAC               
TthHB8I       362  TCGA                 T^CGA
              740  TCGA                
XmaCI          38  CCCGGG               C^CCGGG
              809  CCCGGG              
XmaI           38  CCCGGG               C^CCGGG
              809  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MboI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        TspRI       
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