Restriction map for pF1KB9348
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AvaI        AviII       AvrII       BamHI       
BbrPI       BbsI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MflI        MluI        MseI        MslI        
MspA1I      MvnI        NaeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccIII        462  TCCGGA               T^CCGGA
AcyI          671  GGCGCC               G[GA]^CG[CT]C
AhdI          411  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           10  CCTGAGG              CC^T[ACGT]AGG
ApaI          811  GGGCCC               GGGCC^C
AspEI         411  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI           49  TGGCCA               TGG^CCA
BanI          671  GGCGCC               G^G[CT][GA]CC
BbeI          671  GGCGCC               GGCGC^C
BbvCI         535  GCTGAGG              CCTCAGC(-5/-2)
BcgI          776  GCAAGACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          563  TGATCA               T^GATCA
BfaI          292  CTAG                 C^TAG
BsaBI         690  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         671  GGCGCC               G[GA]^CG[CT]C
BsaI          799  GAGACC               GGTCTC(1/5)
BsaWI         462  TCCGGA               [AT]^CCGG[AT]
BseAI         462  TCCGGA               T^CCGGA
BspEI         462  TCCGGA               T^CCGGA
BspMI         269  ACCTGC               ACCTGC(4/8)
BstAPI         36  GCACTCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         10  CCTGAGG              CC^T[ACGT]AGG
CfoI          672  GCGC                 GCG^C
DrdI          780  GACCATCGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          122  CCATGG               C^C[GA][CT]GG
Eam1105I      411  GACTCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I         10  CCTGAGG              CC^T[ACGT]AGG
FbaI          563  TGATCA               T^GATCA
HaeII         671  GGCGCC               [GA]GCGC^[CT]
HgaI          626  GACGC                GACGC(5/10)
HhaI          672  GCGC                 GCG^C
Hin1I         671  GGCGCC               G[GA]^CG[CT]C
HinP1I        672  GCGC                 G^CGC
HphI          692  TCACC                GGTGA(8/7)
KasI          671  GGCGCC               G^GCGCC
MaeI          292  CTAG                 C^TAG
MaeII         641  ACGT                 A^CGT
MaeIII        657  GTCAC                ^GT[ACGT]AC
MamI          690  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          432  CAATTG               C^AATTG
MluNI          49  TGGCCA               TGG^CCA
MroI          462  TCCGGA               T^CCGGA
MscI           49  TGGCCA               TGG^CCA
MunI          432  CAATTG               C^AATTG
NarI          671  GGCGCC               GG^CGCC
NcoI          122  CCATGG               C^CATGG
NdeI          173  CATATG               CA^TATG
NspI            5  ACATGC               [GA]CATG^[CT]
PflMI         172  CCATATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          505  GAGCTC               GAGCT^C
ScaI          614  AGTACT               AGT^ACT
SexAI         476  ACCTGGT              A^CC[AT]GGT
SfcI          281  CTGTAG               C^T[GA][CT]AG
SfoI          671  GGCGCC               GGC^GCC
SmlI           19  CTCAAG               C^T[CT][GA]AG
TaqI          425  TCGA                 T^CGA
Tsp45I        657  GTCAC                ^GT[GC]AC
Tsp509I       433  AATT                 ^AATT
TthHB8I       425  TCGA                 T^CGA
Van91I        172  CCATATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AfaI          201  GTAC                 GT^AC
              615  GTAC                
BanII         505  GAGCTC               G[GA]GC[CT]^C
              811  GGGCCC              
BcnI          668  CCCGG                CC^[GC]GG
              699  CCGGG               
DpnII         564  GATC                 ^GATC
              690  GATC                
EaeI           49  TGGCCA               [CT]^GGCC[GA]
              746  TGGCCG              
EcoNI         393  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              711  CCTGGAGAAGG         
EcoT14I        46  CCTTGG               C^C[AT][AT]GG
              122  CCATGG              
MboI          564  GATC                 ^GATC
              690  GATC                
NciI          668  CCCGG                CC^[GC]GG
              699  CCGGG               
NdeII         564  GATC                 ^GATC
              690  GATC                
PleI          411  GACTC                GAGTC(4/5)
              304  GAGTC               
PpuMI          14  AGGTCCT              [GA]G^G[AT]CC[CT]
              542  AGGACCT             
RsaI          201  GTAC                 GT^AC
              615  GTAC                
SapI          314  GCTCTTC              GCTCTTC(1/4)
              507  GCTCTTC             
Sau3AI        564  GATC                 ^GATC
              690  GATC                
StyI           46  CCTTGG               C^C[AT][AT]GG
              122  CCATGG              
TspRI         345  CACTG                CA[GC]TG[ACGT][ACGT]^
              489  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BbvI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
Cac8I       Cfr13I      DdeI        DraII       EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
Ksp632I     MboII       MnlI        MspI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       TfiI        TseI        
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