Restriction map for pF1KB9348
 (Linear) (Six-base) MAPSORT of: KIBB9348.seq  Check: 9815  from: 1  to: 819



KIBB9348.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568     819 
   Size:        568     251
 
AhdI GACnn_n'nnGTC

Cuts at:      0     416     819 
   Size:        416     403
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     548     819 
   Size:        548     271
 
ApaI G_GGCC'C

Cuts at:      0     815     819 
   Size:        815       4
 
BanI G'GyrC_C

Cuts at:      0     671     819 
   Size:        671     148
 
BanII G_rGCy'C

Cuts at:      0     509     815     819 
   Size:        509     306       4

  Fragments arranged by size: 

                 509     306       4
 
BbeI G_GCGC'C

Cuts at:      0     675     819 
   Size:        675     144
 
BbvCI CC'TCA_GC

Cuts at:      0     536     819 
   Size:        536     283
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     765     799     819 
   Size:        765      34      20

  Fragments arranged by size: 

                 765      34      20
 
BclI T'GATC_A

Cuts at:      0     563     819 
   Size:        563     256
 
Bme1580I G_kGCm'C

Cuts at:      0     815     819 
   Size:        815       4
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     410     819 
   Size:        410     409
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     428     819 
   Size:        428     391
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     112     217     247     415     471     733     819 
   Size:         52      60     105      30     168      56     262      86

  Fragments arranged by size: 

                 262     168     105      86      60      56      52      30
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     536     819 
   Size:        536     283
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       4     819 
   Size:          4     815
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     793     819 
   Size:        793      26
 
BsaBI GATnn'nnATC

Cuts at:      0     694     819 
   Size:        694     125
 
BsaHI Gr'CG_yC

Cuts at:      0     672     819 
   Size:        672     147
 
BsaWI w'CCGG_w

Cuts at:      0     462     819 
   Size:        462     357
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     330     553     819 
   Size:        109     221     223     266

  Fragments arranged by size: 

                 266     223     221     109
 
BseYI C'CCAG_C

Cuts at:      0     554     819 
   Size:        554     265
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     485     509     819 
   Size:         38     447      24     310

  Fragments arranged by size: 

                 447     310      38      24
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     485     509     815     819 
   Size:         38     447      24     306       4

  Fragments arranged by size: 

                 447     306      38      24       4
 
BspEI T'CCGG_A

Cuts at:      0     462     819 
   Size:        462     357
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     278     819 
   Size:        278     541
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42     819 
   Size:         42     777
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     123     155     258     438     819 
   Size:        123      32     103     180     381

  Fragments arranged by size: 

                 381     180     123     103      32
 
Bsu36I CC'TnA_GG

Cuts at:      0      11     819 
   Size:         11     808
 
BtgI C'CryG_G

Cuts at:      0     122     819 
   Size:        122     697
 
BtsI GCAGTG_nn'

Cuts at:      0     344     819 
   Size:        344     475
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     786     819 
   Size:        786      33
 
EaeI y'GGCC_r

Cuts at:      0      49     746     819 
   Size:         49     697      73

  Fragments arranged by size: 

                 697      73      49
 
EarI CTCTTCn'nnn_

Cuts at:      0      52     321     514     819 
   Size:         52     269     193     305

  Fragments arranged by size: 

                 305     269     193      52
 
EcoICRI GAG'CTC

Cuts at:      0     507     819 
   Size:        507     312
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     112     217     247     415     471     568     733 
   Size:         52      60     105      30     168      56      97     165

Cuts at:    733     819 
   Size:         86

  Fragments arranged by size: 

                 168     165     105      97      86      60      56      52
                  30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     397     715     819 
   Size:        397     318     104

  Fragments arranged by size: 

                 397     318     104
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15     543     812     819 
   Size:         15     528     269       7

  Fragments arranged by size: 

                 528     269      15       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     600     819 
   Size:        600     219
 
HaeII r_GCGC'y

Cuts at:      0     675     819 
   Size:        675     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     201     402     434     819 
   Size:        169      32     201      32     385

  Fragments arranged by size: 

                 385     201     169      32      32
 
KasI G'GCGC_C

Cuts at:      0     671     819 
   Size:        671     148
 
MfeI C'AATT_G

Cuts at:      0     432     819 
   Size:        432     387
 
MscI TGG'CCA

Cuts at:      0      51     819 
   Size:         51     768
 
NarI GG'CG_CC

Cuts at:      0     672     819 
   Size:        672     147
 
NcoI C'CATG_G

Cuts at:      0     122     819 
   Size:        122     697
 
NdeI CA'TA_TG

Cuts at:      0     174     819 
   Size:        174     645
 
NspI r_CATG'y

Cuts at:      0       9     819 
   Size:          9     810
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     178     819 
   Size:        178     641
 
PfoI T'CCnGG_A

Cuts at:      0     710     819 
   Size:        710     109
 
PpuMI rG'GwC_Cy

Cuts at:      0      15     543     819 
   Size:         15     528     276

  Fragments arranged by size: 

                 528     276      15
 
PspOMI G'GGCC_C

Cuts at:      0     811     819 
   Size:        811       8
 
SacI G_AGCT'C

Cuts at:      0     509     819 
   Size:        509     310
 
SapI GCTCTTCn'nnn_

Cuts at:      0     321     514     819 
   Size:        321     193     305

  Fragments arranged by size: 

                 321     305     193
 
ScaI AGT'ACT

Cuts at:      0     616     819 
   Size:        616     203
 
SexAI A'CCwGG_T

Cuts at:      0     476     819 
   Size:        476     343
 
SfcI C'TryA_G

Cuts at:      0     281     819 
   Size:        281     538
 
SfoI GGC'GCC

Cuts at:      0     673     819 
   Size:        673     146
 
SmlI C'TyrA_G

Cuts at:      0      19     819 
   Size:         19     800
 
StyI C'CwwG_G

Cuts at:      0      46     122     819 
   Size:         46      76     697

  Fragments arranged by size: 

                 697      76      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     165     268     819 
   Size:        165     103     551

  Fragments arranged by size: 

                 551     165     103
 
TatI w'GTAC_w

Cuts at:      0     200     614     819 
   Size:        200     414     205

  Fragments arranged by size: 

                 414     205     200
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI     ApaI     BanI    BanII     BbeI    BbvCI
     BcgI     BclI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspEI    BspMI   BstAPI    BstXI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I     KasI     MfeI     MscI     NarI     NcoI     NdeI     NspI
    PflMI     PfoI    PpuMI   PspOMI     SacI     SapI     ScaI    SexAI
     SfcI     SfoI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MluI
     MmeI     MslI   MspA1I     NaeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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