Restriction map for pF1KB9101
 (Linear) (Six-base) MAPSORT of: KIBB9101.seq  Check: 3102  from: 1  to: 3474



KIBB9101.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2385    3474 
   Size:       2385    1089
 
AclI AA'CG_TT

Cuts at:      0    1870    3474 
   Size:       1870    1604
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    3474 
   Size:        394    3080
 
AflII C'TTAA_G

Cuts at:      0    2196    3474 
   Size:       2196    1278
 
AhdI GACnn_n'nnGTC

Cuts at:      0     117    2131    3474 
   Size:        117    2014    1343

  Fragments arranged by size: 

                2014    1343     117
 
AleI CACnn'nnGTG

Cuts at:      0    2305    3474 
   Size:       2305    1169
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     455     500    3474 
   Size:        455      45    2974

  Fragments arranged by size: 

                2974     455      45
 
AlwNI CAG_nnn'CTG

Cuts at:      0     103    1360    2080    3079    3474 
   Size:        103    1257     720     999     395

  Fragments arranged by size: 

                1257     999     720     395     103
 
ApaLI G'TGCA_C

Cuts at:      0      31    1853    2308    3474 
   Size:         31    1822     455    1166

  Fragments arranged by size: 

                1822    1166     455      31
 
ApoI r'AATT_y

Cuts at:      0     318    1464    1731    1896    2291    2701    2722    2923 
   Size:        318    1146     267     165     395     410      21     201

Cuts at:   2923    3474 
   Size:        551

  Fragments arranged by size: 

                1146     551     410     395     318     267     201     165
                  21
 
AvaI C'yCGr_G

Cuts at:      0     839    3474 
   Size:        839    2635
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      92     125    3474 
   Size:         92      33    3349

  Fragments arranged by size: 

                3349      92      33
 
BamHI G'GATC_C

Cuts at:      0    1413    3474 
   Size:       1413    2061
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     257    2402    3474 
   Size:        257    2145    1072

  Fragments arranged by size: 

                2145    1072     257
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     820     854    3474 
   Size:        820      34    2620

  Fragments arranged by size: 

                2620     820      34
 
BfrBI ATG'CAT

Cuts at:      0      78    3474 
   Size:         78    3396
 
BlpI GC'TnA_GC

Cuts at:      0    2748    3474 
   Size:       2748     726
 
Bme1580I G_kGCm'C

Cuts at:      0      35    1857    2312    2337    2658    3474 
   Size:         35    1822     455      25     321     816

  Fragments arranged by size: 

                1822     816     455     321      35      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1622    3101    3474 
   Size:       1622    1479     373

  Fragments arranged by size: 

                1622    1479     373
 
BmtI G_CTAG'C

Cuts at:      0     349    1016    3474 
   Size:        349     667    2458

  Fragments arranged by size: 

                2458     667     349
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      39    3474 
   Size:         39    3435
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1631    2122    3474 
   Size:       1631     491    1352

  Fragments arranged by size: 

                1631    1352     491
 
Bpu10I CC'TnA_GC

Cuts at:      0    1169    3044    3474 
   Size:       1169    1875     430

  Fragments arranged by size: 

                1875    1169     430
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    2334    3474 
   Size:        454    1880    1140

  Fragments arranged by size: 

                1880    1140     454
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1694    1938    3474 
   Size:       1694     244    1536

  Fragments arranged by size: 

                1694    1536     244
 
BsaBI GATnn'nnATC

Cuts at:      0     602    1259    3474 
   Size:        602     657    2215

  Fragments arranged by size: 

                2215     657     602
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     644    3474 
   Size:        644    2830
 
BseYI C'CCAG_C

Cuts at:      0     124    1948    2971    3474 
   Size:        124    1824    1023     503

  Fragments arranged by size: 

                1824    1023     503     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    2383    3080    3474 
   Size:        220    2163     697     394

  Fragments arranged by size: 

                2163     697     394     220
 
BsiHKAI G_wGCw'C

Cuts at:      0      35    1657    1857    2312    3050    3474 
   Size:         35    1622     200     455     738     424

  Fragments arranged by size: 

                1622     738     455     424     200      35
 
BsmI GAATG_Cn'

Cuts at:      0     285    1919    3474 
   Size:        285    1634    1555

  Fragments arranged by size: 

                1634    1555     285
 
Bsp1286I G_dGCh'C

Cuts at:      0      35    1657    1857    2312    2337    2658    3050    3474 
   Size:         35    1622     200     455      25     321     392     424

  Fragments arranged by size: 

                1622     455     424     392     321     200      35      25
            
 
BspHI T'CATG_A

Cuts at:      0     314     495     686    3474 
   Size:        314     181     191    2788

  Fragments arranged by size: 

                2788     314     191     181
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1069    2385    3474 
   Size:       1069    1316    1089

  Fragments arranged by size: 

                1316    1089    1069
 
BsrGI T'GTAC_A

Cuts at:      0    2954    3474 
   Size:       2954     520
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     976    1932    3474 
   Size:        976     956    1542

  Fragments arranged by size: 

                1542     976     956
 
BstYI r'GATC_y

Cuts at:      0     223    1413    3474 
   Size:        223    1190    2061

  Fragments arranged by size: 

                2061    1190     223
 
Bsu36I CC'TnA_GG

Cuts at:      0    1556    2423    3474 
   Size:       1556     867    1051

  Fragments arranged by size: 

                1556    1051     867
 
BtsI GCAGTG_nn'

Cuts at:      0     171    1290    3474 
   Size:        171    1119    2184

  Fragments arranged by size: 

                2184    1119     171
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     719     754    3474 
   Size:        719      35    2720

  Fragments arranged by size: 

                2720     719      35
 
DraI TTT'AAA

Cuts at:      0     323     506     854    2403    2610    2727    2942    3474 
   Size:        323     183     348    1549     207     117     215     532

  Fragments arranged by size: 

                1549     532     348     323     215     207     183     117
            
 
DraIII CAC_nnn'GTG

Cuts at:      0    1909    3474 
   Size:       1909    1565
 
EaeI y'GGCC_r

Cuts at:      0     902     921    1515    3474 
   Size:        902      19     594    1959

  Fragments arranged by size: 

                1959     902     594      19
 
EarI CTCTTCn'nnn_

Cuts at:      0       8     131     289    1931    2164    2687    3311    3474 
   Size:          8     123     158    1642     233     523     624     163

  Fragments arranged by size: 

                1642     624     523     233     163     158     123       8
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1631    2122    3474 
   Size:        394    1237     491    1352

  Fragments arranged by size: 

                1352    1237     491     394
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2084    3474 
   Size:       2084    1390
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2077    2675    3118    3474 
   Size:       2077     598     443     356

  Fragments arranged by size: 

                2077     598     443     356
 
EcoRI G'AATT_C

Cuts at:      0    1464    1731    3474 
   Size:       1464     267    1743

  Fragments arranged by size: 

                1743    1464     267
 
EcoRV GAT'ATC

Cuts at:      0    1437    3474 
   Size:       1437    2037
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1513    3340    3386    3474 
   Size:       1513    1827      46      88

  Fragments arranged by size: 

                1827    1513      88      46
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     426     458     473     505    2117    2149 
   Size:        103      32     291      32      15      32    1612      32

Cuts at:   2149    2389    2421    3265    3297    3474 
   Size:        240      32     844      32     177

  Fragments arranged by size: 

                1612     844     291     240     177     103      32      32
                  32      32      32      32      15
 
HincII GTy'rAC

Cuts at:      0     111     360    1964    2068    3474 
   Size:        111     249    1604     104    1406

  Fragments arranged by size: 

                1604    1406     249     111     104
 
HindIII A'AGCT_T

Cuts at:      0     338    2578    2793    3344    3474 
   Size:        338    2240     215     551     130

  Fragments arranged by size: 

                2240     551     338     215     130
 
HpaI GTT'AAC

Cuts at:      0    1964    3474 
   Size:       1964    1510
 
MfeI C'AATT_G

Cuts at:      0    1504    3474 
   Size:       1504    1970
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     645    1331    3474 
   Size:        645     686    2143

  Fragments arranged by size: 

                2143     686     645
 
MscI TGG'CCA

Cuts at:      0     904     923    1517    3474 
   Size:        904      19     594    1957

  Fragments arranged by size: 

                1957     904     594      19
 
MslI CAynn'nnrTG

Cuts at:      0     974    1523    1571    1930    2305    3474 
   Size:        974     549      48     359     375    1169

  Fragments arranged by size: 

                1169     974     549     375     359      48
 
MspA1I CmG'CkG

Cuts at:      0    1959    2814    3474 
   Size:       1959     855     660

  Fragments arranged by size: 

                1959     855     660
 
NdeI CA'TA_TG

Cuts at:      0    1743    3383    3474 
   Size:       1743    1640      91

  Fragments arranged by size: 

                1743    1640      91
 
NheI G'CTAG_C

Cuts at:      0     345    1012    3474 
   Size:        345     667    2462

  Fragments arranged by size: 

                2462     667     345
 
NsiI A_TGCA'T

Cuts at:      0      80    3474 
   Size:         80    3394
 
NspI r_CATG'y

Cuts at:      0     234    3213    3474 
   Size:        234    2979     261

  Fragments arranged by size: 

                2979     261     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1180    1797    3079    3128    3474 
   Size:       1180     617    1282      49     346

  Fragments arranged by size: 

                1282    1180     617     346      49
 
PpuMI rG'GwC_Cy

Cuts at:      0    2077    2675    3118    3474 
   Size:       2077     598     443     356

  Fragments arranged by size: 

                2077     598     443     356
 
PshAI GACnn'nnGTC

Cuts at:      0    2510    3474 
   Size:       2510     964
 
PsiI TTA'TAA

Cuts at:      0    1085    3474 
   Size:       1085    2389
 
PstI C_TGCA'G

Cuts at:      0     434    1753    3336    3474 
   Size:        434    1319    1583     138

  Fragments arranged by size: 

                1583    1319     434     138
 
PvuII CAG'CTG

Cuts at:      0    1959    2814    3474 
   Size:       1959     855     660

  Fragments arranged by size: 

                1959     855     660
 
SapI GCTCTTCn'nnn_

Cuts at:      0       8    1931    2687    3474 
   Size:          8    1923     756     787

  Fragments arranged by size: 

                1923     787     756       8
 
ScaI AGT'ACT

Cuts at:      0    1755    2211    2666    3474 
   Size:       1755     456     455     808

  Fragments arranged by size: 

                1755     808     456     455
 
SfcI C'TryA_G

Cuts at:      0     430    1028    1749    3332    3474 
   Size:        430     598     721    1583     142

  Fragments arranged by size: 

                1583     721     598     430     142
 
SmlI C'TyrA_G

Cuts at:      0     469     839    2196    2313    3474 
   Size:        469     370    1357     117    1161

  Fragments arranged by size: 

                1357    1161     469     370     117
 
SphI G_CATG'C

Cuts at:      0     234    3474 
   Size:        234    3240
 
SspI AAT'ATT

Cuts at:      0    1249    3474 
   Size:       1249    2225
 
SwaI ATTT'AAAT

Cuts at:      0    2727    3474 
   Size:       2727     747
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    3083    3474 
   Size:       3083     391
 
TatI w'GTAC_w

Cuts at:      0    1225    1563    1630    1753    2209    2664    2954    3474 
   Size:       1225     338      67     123     456     455     290     520

  Fragments arranged by size: 

                1225     520     456     455     338     290     123      67
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     511     543    1398    1430    3474 
   Size:        511      32     855      32    2044

  Fragments arranged by size: 

                2044     855     511      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1187    3474 
   Size:       1187    2287
 
XbaI T'CTAG_A

Cuts at:      0    2538    3067    3474 
   Size:       2538     529     407

  Fragments arranged by size: 

                2538     529     407
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     931    2979    3474 
   Size:        931    2048     495

  Fragments arranged by size: 

                2048     931     495
 
XhoI C'TCGA_G

Cuts at:      0     839    3474 
   Size:        839    2635
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AflII     AhdI     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI     BaeI    BamHI     BbsI     BcgI    BfrBI
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrGI    BstXI    BstYI   Bsu36I     BtsI    CspCI
     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MscI     MslI   MspA1I     NdeI     NheI     NsiI     NspI    PflMI
    PpuMI    PshAI     PsiI     PstI    PvuII     SapI     ScaI     SfcI
     SmlI     SphI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BanI    BanII     BbeI    BbvCI
    BciVI     BclI     BglI    BglII    BmgBI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI
     ClaI     DrdI     EagI     EciI  EcoICRI     FseI     FspI    FspAI
    HaeII     KasI     KpnI     MluI     NaeI     NarI     NcoI   NgoMIV
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     StyI     XmaI     XmnI
     ZraI
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