Restriction map for pF1KB9025
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstXI       BstZ17I     Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO109I    EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MluI        MroI        MslI        MspA1I      MspI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NsiI        NspV        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          625  GTAGAC               GT^[AC][GT]AC
AccII         945  CGCG                 CG^CG
AciI          442  GCGG                 CCGC(-3/-1)
AlwI          646  GATCC                GGATC(4/5)
AocI          223  CCTCAGG              CC^T[ACGT]AGG
AspHI        1111  GAGCAC               G[AT]GC[AT]^C
AspI          835  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII        1142  GGTCC                G^G[AT]CC
BalI         1167  TGGCCA               TGG^CCA
BanI            8  GGTGCC               G^G[CT][GA]CC
BanII        1214  GGGCTC               G[GA]GC[CT]^C
BbvI         1134  GCAGC                GCAGC(8/12)
BfaI         1058  CTAG                 C^TAG
BglII         453  AGATCT               A^GATCT
BsiHKAI      1111  GAGCAC               G[AT]GC[AT]^C
BspHI         345  TCATGA               T^CATGA
BspMI         807  ACCTGC               ACCTGC(4/8)
BssSI        1079  CTCGTG               CACGAG(-5/-1)
BstAPI        670  GCAGAAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        296  GGATG                GGATG(2/0)
BstUI         945  CGCG                 CG^CG
BstYI         453  AGATCT               [GA]^GATC[CT]
Bsu36I        223  CCTCAGG              CC^T[ACGT]AGG
Cfr13I       1142  GGTCC                G^G[ACGT]CC
DraI          525  TTTAAA               TTT^AAA
DrdI          131  GACTGAAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1167  TGGCCA               [CT]^GGCC[GA]
EarI          135  GAAGAG               CTCTTC(1/4)
Eco81I        223  CCTCAGG              CC^T[ACGT]AGG
EcoNI         793  CCTTATATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         106  GAATTC               G^AATTC
Fnu4HI       1134  GCAGC                GC^[ACGT]GC
FokI          296  GGATG                GGATG(9/13)
ItaI         1134  GCAGC                GC^[ACGT]GC
Ksp632I       135  GAAGAG               CTCTTC(1/4)
MaeI         1058  CTAG                 C^TAG
MfeI          665  CAATTG               C^AATTG
MflI          453  AGATCT               [GA]^GATC[CT]
MluNI        1167  TGGCCA               TGG^CCA
MscI         1167  TGGCCA               TGG^CCA
MunI          665  CAATTG               C^AATTG
MvnI          945  CGCG                 CG^CG
NruI          944  TCGCGA               TCG^CGA
PacI          997  TTAATTAA             TTAAT^TAA
PflFI         835  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          345  TCATGA               T^CATGA
Sau96I       1142  GGTCC                G^G[ACGT]CC
SexAI         633  ACCTGGT              A^CC[AT]GGT
TseI         1134  GCAGC                G^C[AT]GC
Tth111I       835  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         453  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          904  GTAC                 GT^AC
             1101  GTAC                
BbsI          398  GAAGAC               GAAGAC(2/6)
             1296  GAAGAC              
BbvCI           3  GCTGAGG              CCTCAGC(-5/-2)
              406  GCTGAGG             
BmyI         1111  GAGCAC               G[AGT]GC[ACT]^C
             1214  GGGCTC              
BpuAI         398  GAAGAC               GAAGAC(2/6)
             1296  GAAGAC              
BsaJI         963  CCTTGG               C^C[ACGT][ACGT]GG
             1104  CCAAGG              
BsiYI         793  CCTTATATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1159  CCTGTAAGTGG         
BslI          793  CCTTATATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1159  CCTGTAAGTGG         
Bsp1286I     1111  GAGCAC               G[AGT]GC[ACT]^C
             1214  GGGCTC              
BssKI         338  CCAGG                ^CC[ACGT]GG
              634  CCTGG               
BstNI         338  CCAGG                CC^[AT]GG
              634  CCTGG               
EcoRII        338  CCAGG                ^CC[AT]GG
              634  CCTGG               
EcoT14I       963  CCTTGG               C^C[AT][AT]GG
             1104  CCAAGG              
MvaI          338  CCAGG                CC^[AT]GG
              634  CCTGG               
NspI          402  ACATGC               [GA]CATG^[CT]
              555  ACATGC              
PleI          879  GACTC                GAGTC(4/5)
              138  GAGTC               
RsaI          904  GTAC                 GT^AC
             1101  GTAC                
ScrFI         338  CCAGG                CC^[ACGT]GG
              634  CCTGG               
SfaNI         439  GATGC                GCATC(5/9)
             1069  GATGC               
StyI          963  CCTTGG               C^C[AT][AT]GG
             1104  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AcsI        ApoI        BseRI       BsmAI       BsrI        
BstEII      BstPI       DdeI        DpnII       EcoO65I     
HaeIII      HinfI       HphI        MaeIII      MboI        
MboII       MnlI        MseI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       
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