Restriction map for pF1KB7005
##################################
# NO CUT ENZYMES (   64 enzymes) #
##################################

Acc65I      AclI        AflII       Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     BalI        
BamHI       BbeI        BcgI        BfrI        BglI        
BsiWI       BsmBI       BspHI       BspLU11I    BstBI       
CpoI        DrdI        Eco47III    EcoRI       EcoRV       
HaeII       HpaI        KasI        KpnI        KspI        
MfeI        MluI        MluNI       MscI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SbfI        ScaI        SfiI        SfoI        
SfuI        SmaI        SnaBI       SphI        Sse8387I    
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII       2561  TCCGGA               T^CCGGA
AflIII       2336  ACGTGT               A^C[GA][CT]GT
AgeI         2944  ACCGGT               A^CCGGT
AhdI         3559  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        942  GTGCAC               G^TGCAC
ApaLI         942  GTGCAC               G^TGCAC
Asp700I       537  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3559  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         917  TGCGCA               TGC^GCA
AvrII         591  CCTAGG               C^CTAGG
BciVI        1996  GTATCC               GTATCC(6/5)
BclI         3254  TGATCA               T^GATCA
BlnI          591  CCTAGG               C^CTAGG
BsaBI        2926  GATAGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1978  GAGACC               GGTCTC(1/5)
BseAI        2561  TCCGGA               T^CCGGA
BsiEI        3689  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1835  TGTACA               T^GTACA
BspDI         882  ATCGAT               AT^CGAT
BspEI        2561  TCCGGA               T^CCGGA
BsrBI        3451  CCGCTC               CCGCTC(-3/-3)
BsrGI        1835  TGTACA               T^GTACA
BssHII        987  GCGCGC               G^CGCGC
BstAPI       3678  GCAGGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2751  GGTTACC              G^GT[ACGT]ACC
BstPI        2751  GGTTACC              G^GT[ACGT]ACC
ClaI          882  ATCGAT               AT^CGAT
DraI         3148  TTTAAA               TTT^AAA
EagI         3689  CGGCCG               C^GGCCG
Eam1105I     3559  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3689  CGGCCG               C^GGCCG
Eco52I       3689  CGGCCG               C^GGCCG
EcoO65I      2751  GGTTACC              G^GT[ACGT]ACC
EcoT22I      1744  ATGCAT               ATGCA^T
FbaI         3254  TGATCA               T^GATCA
FseI         3690  GGCCGGCC             GGCCGG^CC
FspI          917  TGCGCA               TGC^GCA
MamI         2926  GATAGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2561  TCCGGA               T^CCGGA
NcoI           41  CCATGG               C^CATGG
NsiI         1744  ATGCAT               ATGCA^T
PinAI        2944  ACCGGT               A^CCGGT
PshAI        1211  GACTTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI         3349  GTCGAC               G^TCGAC
SexAI        1050  ACCTGGT              A^CC[AT]GGT
SgrAI          26  CGCCGGTG             C[GA]^CCGG[CT]G
SpeI         2967  ACTAGT               A^CTAGT
SspBI        1835  TGTACA               T^GTACA
SspI          662  AATATT               AAT^ATT
StuI         3657  AGGCCT               AGG^CCT
XbaI         2133  TCTAGA               T^CTAGA
XcmI          823  CCAGTGAAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          537  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII        1947  GACGTC               GACGT^C
             3481  GACGTC              
AocI         2144  CCTCAGG              CC^T[ACGT]AGG
             2858  CCTCAGG             
AspI         2578  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3751  GACCACGTC           
BanI          793  GGTGCC               G^G[CT][GA]CC
             2167  GGTGCC              
BbvCI        1531  GCTGAGG              CCTCAGC(-5/-2)
             2178  GCTGAGG             
BlpI         1282  GCTGAGC              GC^T[ACGT]AGC
             2466  GCTTAGC             
Bpu1102I     1282  GCTGAGC              GC^T[ACGT]AGC
             2466  GCTTAGC             
Bsu36I       2144  CCTCAGG              CC^T[ACGT]AGG
             2858  CCTCAGG             
CelII        1282  GCTGAGC              GC^T[ACGT]AGC
             2466  GCTTAGC             
DraII         148  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1227  AGGACCT             
Eco81I       2144  CCTCAGG              CC^T[ACGT]AGG
             2858  CCTCAGG             
EcoO109I      148  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1227  AGGACCT             
FauI         2014  CCCGC                CCCGC(4/6)
              876  GCGGG               
HindIII      1355  AAGCTT               A^AGCTT
             3331  AAGCTT              
NaeI         3687  GCCGGC               GCC^GGC
             3691  GCCGGC              
NgoMIV       3687  GCCGGC               G^CCGGC
             3691  GCCGGC              
NspI         2099  ACATGC               [GA]CATG^[CT]
             2687  ACATGC              
PflFI        2578  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3751  GACCACGTC           
PflMI        1149  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3753  CCACGTCCTGG         
PpuMI         148  AGGTCCT              [GA]G^G[AT]CC[CT]
             1227  AGGACCT             
Tth111I      2578  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3751  GACCACGTC           
Van91I       1149  CCACACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3753  CCACGTCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApoI        
AspHI       AvaI        AvaII       BanII       BbrPI       
BbsI        BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaJI       BsaWI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BssSI       Bst1107I    BstF5I      
BstNI       BstUI       BstXI       BstYI       BstZ17I     
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PmaCI       PmlI        PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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