Restriction map for pF1KB7005
 (Linear) (Six-base) MAPSORT of: KIBB7005.seq  Check: 3092  from: 1  to: 3777



KIBB7005.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AatII G_ACGT'C

Cuts at:      0    1951    3485    3777 
   Size:       1951    1534     292

  Fragments arranged by size: 

                1951    1534     292
 
AccI GT'mk_AC

Cuts at:      0    1405    2257    2522    3136    3350    3572    3777 
   Size:       1405     852     265     614     214     222     205

  Fragments arranged by size: 

                1405     852     614     265     222     214     205
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364    1045    1676    1875    2642    2805    2817    3777 
   Size:        364     681     631     199     767     163      12     960

  Fragments arranged by size: 

                 960     767     681     631     364     199     163      12
            
 
AflIII A'CryG_T

Cuts at:      0    2336    3777 
   Size:       2336    1441
 
AgeI A'CCGG_T

Cuts at:      0    2944    3777 
   Size:       2944     833
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3564    3777 
   Size:       3564     213
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     967    1769    2614    3777 
   Size:        967     802     845    1163

  Fragments arranged by size: 

                1163     967     845     802
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2971    3003    3777 
   Size:       2971      32     774

  Fragments arranged by size: 

                2971     774      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1023    3103    3412    3684    3777 
   Size:       1023    2080     309     272      93

  Fragments arranged by size: 

                2080    1023     309     272      93
 
ApaLI G'TGCA_C

Cuts at:      0     942    3777 
   Size:        942    2835
 
ApoI r'AATT_y

Cuts at:      0      81    2629    2895    3145    3159    3777 
   Size:         81    2548     266     250      14     618

  Fragments arranged by size: 

                2548     618     266     250      81      14
 
AvaI C'yCGr_G

Cuts at:      0    1975    3416    3714    3777 
   Size:       1975    1441     298      63

  Fragments arranged by size: 

                1975    1441     298      63
 
AvrII C'CTAG_G

Cuts at:      0     591    3777 
   Size:        591    3186
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     278     311    3777 
   Size:        278      33    3466

  Fragments arranged by size: 

                3466     278      33
 
BanI G'GyrC_C

Cuts at:      0     793    2167    3777 
   Size:        793    1374    1610

  Fragments arranged by size: 

                1610    1374     793
 
BanII G_rGCy'C

Cuts at:      0     165    2290    2354    2708    3777 
   Size:        165    2125      64     354    1069

  Fragments arranged by size: 

                2125    1069     354     165      64
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1924    2537    3693    3777 
   Size:       1924     613    1156      84

  Fragments arranged by size: 

                1924    1156     613      84
 
BbvCI CC'TCA_GC

Cuts at:      0    1532    2179    3777 
   Size:       1532     647    1598

  Fragments arranged by size: 

                1598    1532     647
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2007    3777 
   Size:       2007    1770
 
BclI T'GATC_A

Cuts at:      0    3254    3777 
   Size:       3254     523
 
BfrBI ATG'CAT

Cuts at:      0    1746    3777 
   Size:       1746    2031
 
BglII A'GATC_T

Cuts at:      0     912    1242    1449    1767    3191    3777 
   Size:        912     330     207     318    1424     586

  Fragments arranged by size: 

                1424     912     586     330     318     207
 
BlpI GC'TnA_GC

Cuts at:      0    1283    2467    3777 
   Size:       1283    1184    1310

  Fragments arranged by size: 

                1310    1283    1184
 
Bme1580I G_kGCm'C

Cuts at:      0     946    1128    1545    2172    3721    3777 
   Size:        946     182     417     627    1549      56

  Fragments arranged by size: 

                1549     946     627     417     182      56
 
BmgBI CAC'GTC

Cuts at:      0    2337    3756    3777 
   Size:       2337    1419      21

  Fragments arranged by size: 

                2337    1419      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2084    3375    3777 
   Size:       2084    1291     402

  Fragments arranged by size: 

                2084    1291     402
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2142    2160    3777 
   Size:       2142      18    1617

  Fragments arranged by size: 

                2142    1617      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     669    1108    2041    2179    3265    3693    3777 
   Size:         92     577     439     933     138    1086     428      84

  Fragments arranged by size: 

                1086     933     577     439     428     138      92      84
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1532    2179    3777 
   Size:       1532     647    1598

  Fragments arranged by size: 

                1598    1532     647
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    3022    3595    3777 
   Size:        642    2380     573     182

  Fragments arranged by size: 

                2380     642     573     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1972    3777 
   Size:       1972    1805
 
BsaAI yAC'GTr

Cuts at:      0    1803    1863    2175    2193    2364    2622    3777 
   Size:       1803      60     312      18     171     258    1155

  Fragments arranged by size: 

                1803    1155     312     258     171      60      18
 
BsaBI GATnn'nnATC

Cuts at:      0    2930    3777 
   Size:       2930     847
 
BsaHI Gr'CG_yC

Cuts at:      0       5     722     749    1948    3482    3777 
   Size:          5     717      27    1199    1534     295

  Fragments arranged by size: 

                1534    1199     717     295      27       5
 
BsaWI w'CCGG_w

Cuts at:      0    1913    2561    2944    3777 
   Size:       1913     648     383     833

  Fragments arranged by size: 

                1913     833     648     383
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     642     672    2834    2864    3777 
   Size:        642      30    2162      30     913

  Fragments arranged by size: 

                2162     913     642      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     223     694    1675    2118    2176    3513    3777 
   Size:         28     195     471     981     443      58    1337     264

  Fragments arranged by size: 

                1337     981     471     443     264     195      58      28
            
 
BseYI C'CCAG_C

Cuts at:      0     983    1067    1538    2354    2664    2691    2990    3096 
   Size:        983      84     471     816     310      27     299     106

Cuts at:   3096    3739    3777 
   Size:        643      38

  Fragments arranged by size: 

                 983     816     643     471     310     299     106      84
                  38      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751     988    1036    2205    2344    2347    3500    3777 
   Size:        751     237      48    1169     139       3    1153     277

  Fragments arranged by size: 

                1169    1153     751     277     237     139      48       3
            
 
BsiEI CG_ry'CG

Cuts at:      0    3692    3777 
   Size:       3692      85
 
BsiHKAI G_wGCw'C

Cuts at:      0     494     946    1059    1515    3653    3777 
   Size:        494     452     113     456    2138     124

  Fragments arranged by size: 

                2138     494     456     452     124     113
 
BsmI GAATG_Cn'

Cuts at:      0     543    1746    2396    3630    3777 
   Size:        543    1203     650    1234     147

  Fragments arranged by size: 

                1234    1203     650     543     147
 
Bsp1286I G_dGCh'C

Cuts at:      0     165     494     946    1059    1128    1515    1545    2172 
   Size:        165     329     452     113      69     387      30     627

Cuts at:   2172    2290    2354    2708    3653    3721    3777 
   Size:        118      64     354     945      68      56

  Fragments arranged by size: 

                 945     627     452     387     354     329     165     118
                 113      69      68      64      56      30
 
BspEI T'CCGG_A

Cuts at:      0    2561    3777 
   Size:       2561    1216
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1261    1622    2969    3777 
   Size:       1261     361    1347     808

  Fragments arranged by size: 

                1347    1261     808     361
 
BsrBI CCG'CTC

Cuts at:      0    3453    3777 
   Size:       3453     324
 
BsrDI GCAATG_nn'

Cuts at:      0     562    1142    2478    3069    3777 
   Size:        562     580    1336     591     708

  Fragments arranged by size: 

                1336     708     591     580     562
 
BsrFI r'CCGG_y

Cuts at:      0      27    2428    2944    3074    3687    3691    3777 
   Size:         27    2401     516     130     613       4      86

  Fragments arranged by size: 

                2401     613     516     130      86      27       4
 
BsrGI T'GTAC_A

Cuts at:      0    1835    3777 
   Size:       1835    1942
 
BssHII G'CGCG_C

Cuts at:      0     987    3777 
   Size:        987    2790
 
BssSI C'ACGA_G

Cuts at:      0    2532    2586    3258    3777 
   Size:       2532      54     672     519

  Fragments arranged by size: 

                2532     672     519      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3684    3777 
   Size:       3684      93
 
BstEII G'GTnAC_C

Cuts at:      0    2751    3777 
   Size:       2751    1026
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     140     867    1794    3408    3777 
   Size:        140     727     927    1614     369

  Fragments arranged by size: 

                1614     927     727     369     140
 
BstYI r'GATC_y

Cuts at:      0     912    1242    1449    1767    3191    3777 
   Size:        912     330     207     318    1424     586

  Fragments arranged by size: 

                1424     912     586     330     318     207
 
BstZ17I GTA'TAC

Cuts at:      0    1406    2258    3137    3777 
   Size:       1406     852     879     640

  Fragments arranged by size: 

                1406     879     852     640
 
Bsu36I CC'TnA_GG

Cuts at:      0    2145    2859    3777 
   Size:       2145     714     918

  Fragments arranged by size: 

                2145     918     714
 
BtgI C'CryG_G

Cuts at:      0      41     970    1793    2785    3777 
   Size:         41     929     823     992     992

  Fragments arranged by size: 

                 992     992     929     823      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2793    3777 
   Size:       2793     984
 
BtsI GCAGTG_nn'

Cuts at:      0    3377    3682    3777 
   Size:       3377     305      95

  Fragments arranged by size: 

                3377     305      95
 
ClaI AT'CG_AT

Cuts at:      0     883    3777 
   Size:        883    2894
 
DraI TTT'AAA

Cuts at:      0    3150    3777 
   Size:       3150     627
 
DraIII CAC_nnn'GTG

Cuts at:      0     329    1054    1155    2769    3777 
   Size:        329     725     101    1614    1008

  Fragments arranged by size: 

                1614    1008     725     329     101
 
EaeI y'GGCC_r

Cuts at:      0     156    1574    2430    2739    3689    3693    3777 
   Size:        156    1418     856     309     950       4      84

  Fragments arranged by size: 

                1418     950     856     309     156      84       4
 
EagI C'GGCC_G

Cuts at:      0    3689    3777 
   Size:       3689      88
 
EarI CTCTTCn'nnn_

Cuts at:      0     519     625     760     823     961    1652    3044    3460 
   Size:        519     106     135      63     138     691    1392     416

Cuts at:   3460    3777 
   Size:        317

  Fragments arranged by size: 

                1392     691     519     416     317     138     135     106
                  63
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2518    3777 
   Size:       2518    1259
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     364     669    1045    1108    1676    1875    2041 
   Size:         92     272     305     376      63     568     199     166

Cuts at:   2041    2179    2642    2805    2817    3265    3693    3777 
   Size:        138     463     163      12     448     428      84

  Fragments arranged by size: 

                 568     463     448     428     376     305     272     199
                 166     163     138      92      84      63      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1531    2497    3277    3777 
   Size:       1531     966     780     500

  Fragments arranged by size: 

                1531     966     780     500
 
EcoO109I rG'GnC_Cy

Cuts at:      0     149    1228    3777 
   Size:        149    1079    2549

  Fragments arranged by size: 

                2549    1079     149
 
FseI GG_CCGG'CC

Cuts at:      0    3695    3777 
   Size:       3695      82
 
FspI TGC'GCA

Cuts at:      0     919    3777 
   Size:        919    2858
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     833     865    2326    2358    2580    2612    3550    3582 
   Size:        833      32    1461      32     222      32     938      32

Cuts at:   3582    3777 
   Size:        195

  Fragments arranged by size: 

                1461     938     833     222     195      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     774    1260    3351    3777 
   Size:        774     486    2091     426

  Fragments arranged by size: 

                2091     774     486     426
 
HindIII A'AGCT_T

Cuts at:      0    1355    3331    3777 
   Size:       1355    1976     446

  Fragments arranged by size: 

                1976    1355     446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     521    1422    2552    2600    3066    3777 
   Size:        127     394     901    1130      48     466     711

  Fragments arranged by size: 

                1130     901     711     466     394     127      48
 
MslI CAynn'nnrTG

Cuts at:      0     865    1399    3400    3406    3442    3777 
   Size:        865     534    2001       6      36     335

  Fragments arranged by size: 

                2001     865     534     335      36       6
 
MspA1I CmG'CkG

Cuts at:      0      21    1572    2271    2358    2373    2744    2919    3114 
   Size:         21    1551     699      87      15     371     175     195

Cuts at:   3114    3545    3777 
   Size:        431     232

  Fragments arranged by size: 

                1551     699     431     371     232     195     175      87
                  21      15
 
NaeI GCC'GGC

Cuts at:      0    3689    3693    3777 
   Size:       3689       4      84

  Fragments arranged by size: 

                3689      84       4
 
NcoI C'CATG_G

Cuts at:      0      41    3777 
   Size:         41    3736
 
NgoMIV G'CCGG_C

Cuts at:      0    3687    3691    3777 
   Size:       3687       4      86

  Fragments arranged by size: 

                3687      86       4
 
NsiI A_TGCA'T

Cuts at:      0    1748    3777 
   Size:       1748    2029
 
NspI r_CATG'y

Cuts at:      0    2103    2691    3777 
   Size:       2103     588    1086

  Fragments arranged by size: 

                2103    1086     588
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1155    3759    3777 
   Size:       1155    2604      18

  Fragments arranged by size: 

                2604    1155      18
 
PfoI T'CCnGG_A

Cuts at:      0     532    3758    3777 
   Size:        532    3226      19

  Fragments arranged by size: 

                3226     532      19
 
PmlI CAC'GTG

Cuts at:      0    1803    1863    2175    2193    2364    2622    3777 
   Size:       1803      60     312      18     171     258    1155

  Fragments arranged by size: 

                1803    1155     312     258     171      60      18
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1013    1045    3777 
   Size:       1013      32    2732

  Fragments arranged by size: 

                2732    1013      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     149    1228    3777 
   Size:        149    1079    2549

  Fragments arranged by size: 

                2549    1079     149
 
PshAI GACnn'nnGTC

Cuts at:      0    1215    3777 
   Size:       1215    2562
 
PsiI TTA'TAA

Cuts at:      0    1322    3777 
   Size:       1322    2455
 
PstI C_TGCA'G

Cuts at:      0     710     965    1619    2089    2465    3643    3680    3777 
   Size:        710     255     654     470     376    1178      37      97

  Fragments arranged by size: 

                1178     710     654     470     376     255      97      37
            
 
PvuII CAG'CTG

Cuts at:      0    1572    2271    2358    2919    3114    3545    3777 
   Size:       1572     699      87     561     195     431     232

  Fragments arranged by size: 

                1572     699     561     431     232     195      87
 
SalI G'TCGA_C

Cuts at:      0    3349    3777 
   Size:       3349     428
 
SapI GCTCTTCn'nnn_

Cuts at:      0     823     961    3044    3460    3777 
   Size:        823     138    2083     416     317

  Fragments arranged by size: 

                2083     823     416     317     138
 
SexAI A'CCwGG_T

Cuts at:      0    1050    3777 
   Size:       1050    2727
 
SfcI C'TryA_G

Cuts at:      0     305     706     961    1615    2085    2461    3003    3639 
   Size:        305     401     255     654     470     376     542     636

Cuts at:   3639    3676    3777 
   Size:         37     101

  Fragments arranged by size: 

                 654     636     542     470     401     376     305     255
                 101      37
 
SgrAI Cr'CCGG_yG

Cuts at:      0      27    3777 
   Size:         27    3750
 
SmlI C'TyrA_G

Cuts at:      0     621    3037    3610    3777 
   Size:        621    2416     573     167

  Fragments arranged by size: 

                2416     621     573     167
 
SpeI A'CTAG_T

Cuts at:      0    2967    3777 
   Size:       2967     810
 
SspI AAT'ATT

Cuts at:      0     664    3777 
   Size:        664    3113
 
StuI AGG'CCT

Cuts at:      0    3659    3777 
   Size:       3659     118
 
StyI C'CwwG_G

Cuts at:      0      41     591    1193    1593    2240    3777 
   Size:         41     550     602     400     647    1537

  Fragments arranged by size: 

                1537     647     602     550     400      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1059    3777 
   Size:       1059    2718
 
TatI w'GTAC_w

Cuts at:      0    1835    2339    3777 
   Size:       1835     504    1438

  Fragments arranged by size: 

                1835    1438     504
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     642     674    1933    1965    2834    2866    3025    3057 
   Size:        642      32    1259      32     869      32     159      32

Cuts at:   3057    3777 
   Size:        720

  Fragments arranged by size: 

                1259     869     720     642     159      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2581    3754    3777 
   Size:       2581    1173      23

  Fragments arranged by size: 

                2581    1173      23
 
XbaI T'CTAG_A

Cuts at:      0    2133    3777 
   Size:       2133    1644
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     830    3777 
   Size:        830    2947
 
XmnI GAAnn'nnTTC

Cuts at:      0     541    3777 
   Size:        541    3236
 
ZraI GAC'GTC

Cuts at:      0    1949    3483    3777 
   Size:       1949    1534     294

  Fragments arranged by size: 

                1949    1534     294
 

 Enzymes that do cut:

    AatII     AccI     AcuI   AflIII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     ApoI     AvaI    AvrII     BaeI     BanI    BanII
     BbsI    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FseI     FspI
    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NsiI     NspI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI     PsiI     PstI    PvuII     SalI     SapI    SexAI     SfcI
    SgrAI     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI    AflII     AleI     ApaI     AscI
     AseI    AsiSI    BamHI     BbeI     BcgI     BglI     BmtI    BsiWI
    BsmBI    BspHI    BstBI    CspCI     DrdI  EcoICRI    EcoRI    EcoRV
     FalI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NarI     NdeI     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SphI     SrfI     SwaI     XhoI     XmaI
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