################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccII AflII AgeI AhdI ApaI AscI AspEI AspI AviII BbeI BbrPI BbvCI BfrI BglI BsiEI BsiWI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I FseI FspI KasI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI Sse8387I Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 5474 GACGTC GACGT^C Acc65I 1383 GGTACC G^GTACC AccIII 4835 TCCGGA T^CCGGA AclI 7172 AACGTT AA^CGTT AcyI 5474 GACGTC G[GA]^CG[CT]C AocI 4110 CCTTAGG CC^T[ACGT]AGG Aor51HI 77 AGCGCT AGC^GCT Asp718I 1383 GGTACC G^GTACC AvaI 692 CTCGAG C^[CT]CG[GA]G AvrII 739 CCTAGG C^CTAGG BlnI 739 CCTAGG C^CTAGG BlpI 7309 GCTAAGC GC^T[ACGT]AGC Bpu1102I 7309 GCTAAGC GC^T[ACGT]AGC BsaAI 5234 TACGTA [CT]AC^GT[GA] BsaHI 5474 GACGTC G[GA]^CG[CT]C BseAI 4835 TCCGGA T^CCGGA BsmBI 5472 GAGACG CGTCTC(1/5) BsoBI 692 CTCGAG C^[CT]CG[GA]G BspDI 5254 ATCGAT AT^CGAT BspEI 4835 TCCGGA T^CCGGA BssSI 8099 CTCGTG CACGAG(-5/-1) Bsu36I 4110 CCTTAGG CC^T[ACGT]AGG CelII 7309 GCTAAGC GC^T[ACGT]AGC CfoI 78 GCGC GCG^C ClaI 5254 ATCGAT AT^CGAT DraIII 10 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 77 AGCGCT AGC^GCT Eco81I 4110 CCTTAGG CC^T[ACGT]AGG EcoNI 6 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 4844 CCCGC CCCGC(4/6) HaeII 77 AGCGCT [GA]GCGC^[CT] HgaI 220 GCGTC GACGC(5/10) HhaI 78 GCGC GCG^C Hin1I 5474 GACGTC G[GA]^CG[CT]C HinP1I 78 GCGC G^CGC HpaI 256 GTTAAC GTT^AAC KpnI 1383 GGTACC GGTAC^C MroI 4835 TCCGGA T^CCGGA PaeR7I 692 CTCGAG C^TCGAG PshAI 8740 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 7172 AACGTT AA^CGTT SapI 6205 GCTCTTC GCTCTTC(1/4) SnaBI 5234 TACGTA TAC^GTA SwaI 443 ATTTAAAT ATTT^AAAT XbaI 3436 TCTAGA T^CTAGA XhoI 692 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Alw44I 3152 GTGCAC G^TGCAC 7088 GTGCAC ApaLI 3152 GTGCAC G^TGCAC 7088 GTGCAC BanI 1383 GGTACC G^G[CT][GA]CC 7230 GGCACC BcgI 1848 CGAGGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 7964 GCATAGCAATCG BciVI 5694 GGATAC GTATCC(6/5) 8604 GGATAC BclI 1425 TGATCA T^GATCA 5397 TGATCA BglII 456 AGATCT A^GATCT 3205 AGATCT BsaI 4151 GAGACC GGTCTC(1/5) 4115 GGTCTC BsaWI 1159 ACCGGA [AT]^CCGG[AT] 4835 TCCGGA BsgI 7 CTGCAC GTGCAG(16/14) 2096 GTGCAG Bsp1407I 1173 TGTACA T^GTACA 6556 TGTACA BsrGI 1173 TGTACA T^GTACA 6556 TGTACA BstXI 6871 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 8652 CCATGACAGTGG FbaI 1425 TGATCA T^GATCA 5397 TGATCA HindIII 6837 AAGCTT A^AGCTT 7712 AAGCTT PpuMI 3272 GGGTCCT [GA]G^G[AT]CC[CT] 7689 AGGTCCT SexAI 11 ACCAGGT A^CC[AT]GGT 3126 ACCTGGT SpeI 5698 ACTAGT A^CTAGT 7143 ACTAGT SspBI 1173 TGTACA T^GTACA 6556 TGTACA StuI 3133 AGGCCT AGG^CCT 4701 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI