Restriction map for pF1KB5691
 (Linear) (Six-base) MAPSORT of: KIBB5691.seq  Check: 3481  from: 1  to: 2580



KIBB5691.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:      0     570    2580 
   Size:        570    2010
 
AclI AA'CG_TT

Cuts at:      0     371    2580 
   Size:        371    2209
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803    1210    1640    1814    1898    1936    2043    2580 
   Size:        803     407     430     174      84      38     107     537

  Fragments arranged by size: 

                 803     537     430     407     174     107      84      38
            
 
AflIII A'CryG_T

Cuts at:      0     412    2580 
   Size:        412    2168
 
AgeI A'CCGG_T

Cuts at:      0    2519    2580 
   Size:       2519      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0     955    2017    2580 
   Size:        955    1062     563

  Fragments arranged by size: 

                1062     955     563
 
ApoI r'AATT_y

Cuts at:      0     106    2580 
   Size:        106    2474
 
AvaI C'yCGr_G

Cuts at:      0       5      55    2580 
   Size:          5      50    2525

  Fragments arranged by size: 

                2525      50       5
 
BanI G'GyrC_C

Cuts at:      0     275    2580 
   Size:        275    2305
 
BanII G_rGCy'C

Cuts at:      0    1402    1579    2580 
   Size:       1402     177    1001

  Fragments arranged by size: 

                1402    1001     177
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     345    2091    2580 
   Size:        345    1746     489

  Fragments arranged by size: 

                1746     489     345
 
BglII A'GATC_T

Cuts at:      0    1102    2357    2580 
   Size:       1102    1255     223

  Fragments arranged by size: 

                1255    1102     223
 
BlpI GC'TnA_GC

Cuts at:      0    1396    2252    2580 
   Size:       1396     856     328

  Fragments arranged by size: 

                1396     856     328
 
Bme1580I G_kGCm'C

Cuts at:      0     278     676    2580 
   Size:        278     398    1904

  Fragments arranged by size: 

                1904     398     278
 
BmgBI CAC'GTC

Cuts at:      0      39     574     584    2580 
   Size:         39     535      10    1996

  Fragments arranged by size: 

                1996     535      39      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1669    1916    1975    2580 
   Size:       1669     247      59     605

  Fragments arranged by size: 

                1669     605     247      59
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     206     365    1193    1301    2139    2580 
   Size:         82     124     159     828     108     838     441

  Fragments arranged by size: 

                 838     828     441     159     124     108      82
 
Bpu10I CC'TnA_GC

Cuts at:      0    1256    1880    2580 
   Size:       1256     624     700

  Fragments arranged by size: 

                1256     700     624
 
BsaAI yAC'GTr

Cuts at:      0     413    2580 
   Size:        413    2167
 
BsaWI w'CCGG_w

Cuts at:      0    2519    2580 
   Size:       2519      61
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      55      85     338     368    1260    1290    1599    1629 
   Size:         55      30     253      30     892      30     309      30

Cuts at:   1629    2580 
   Size:        951

  Fragments arranged by size: 

                 951     892     309     253      55      30      30      30
                  30
 
BseYI C'CCAG_C

Cuts at:      0     669    2580 
   Size:        669    1911
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513    2580 
   Size:        513    2067
 
BsiHKAI G_wGCw'C

Cuts at:      0    1579    2580 
   Size:       1579    1001
 
BsmI GAATG_Cn'

Cuts at:      0     671    2580 
   Size:        671    1909
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     676    1402    1579    2580 
   Size:        278     398     726     177    1001

  Fragments arranged by size: 

                1001     726     398     278     177
 
BspHI T'CATG_A

Cuts at:      0    1920    2580 
   Size:       1920     660
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     688    2580 
   Size:        688    1892
 
BsrDI GCAATG_nn'

Cuts at:      0    1199    1533    2580 
   Size:       1199     334    1047

  Fragments arranged by size: 

                1199    1047     334
 
BsrFI r'CCGG_y

Cuts at:      0    2519    2580 
   Size:       2519      61
 
BsrGI T'GTAC_A

Cuts at:      0     313    2580 
   Size:        313    2267
 
BssSI C'ACGA_G

Cuts at:      0     860    2580 
   Size:        860    1720
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2262    2580 
   Size:       2262     318
 
BstEII G'GTnAC_C

Cuts at:      0    2363    2580 
   Size:       2363     217
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1728    2580 
   Size:       1728     852
 
BstYI r'GATC_y

Cuts at:      0    1102    2357    2580 
   Size:       1102    1255     223

  Fragments arranged by size: 

                1255    1102     223
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2131    2580 
   Size:       2131     449
 
BtsI GCAGTG_nn'

Cuts at:      0    2246    2580 
   Size:       2246     334
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      11      46     570     605    2580 
   Size:         11      35     524      35    1975

  Fragments arranged by size: 

                1975     524      35      35      11
 
EaeI y'GGCC_r

Cuts at:      0    2220    2580 
   Size:       2220     360
 
EarI CTCTTCn'nnn_

Cuts at:      0      96     822     881     888     893    1151    1955    2369 
   Size:         96     726      59       7       5     258     804     414

Cuts at:   2369    2580 
   Size:        211

  Fragments arranged by size: 

                 804     726     414     258     211      96      59       7
                   5
 
EcoICRI GAG'CTC

Cuts at:      0    1577    2580 
   Size:       1577    1003
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     206     365     803    1193    1210    1301    1640 
   Size:         82     124     159     438     390      17      91     339

Cuts at:   1640    1814    1898    1936    2043    2139    2580 
   Size:        174      84      38     107      96     441

  Fragments arranged by size: 

                 441     438     390     339     174     159     124     107
                  96      91      84      82      38      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      50      66    2014    2580 
   Size:         50      16    1948     566

  Fragments arranged by size: 

                1948     566      50      16
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     154    2580 
   Size:        154    2426
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87      95     127    1030    1062    1260    1292 
   Size:         55      32       8      32     903      32     198      32

Cuts at:   1292    1632    1664    1723    1755    2014    2046    2580 
   Size:        340      32      59      32     259      32     534

  Fragments arranged by size: 

                 903     534     340     259     198      59      55      32
                  32      32      32      32      32      32       8
 
HincII GTy'rAC

Cuts at:      0     571    2580 
   Size:        571    2009
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1760    2504    2580 
   Size:        154    1606     744      76

  Fragments arranged by size: 

                1606     744     154      76
 
MscI TGG'CCA

Cuts at:      0    2222    2580 
   Size:       2222     358
 
MslI CAynn'nnrTG

Cuts at:      0     774    2580 
   Size:        774    1806
 
MspA1I CmG'CkG

Cuts at:      0    1213    2580 
   Size:       1213    1367
 
PacI TTA_AT'TAA

Cuts at:      0     563    2580 
   Size:        563    2017
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2183    2580 
   Size:       2183     397
 
PfoI T'CCnGG_A

Cuts at:      0    2155    2580 
   Size:       2155     425
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1873    1905    2580 
   Size:       1873      32     675

  Fragments arranged by size: 

                1873     675      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      50      66    2014    2580 
   Size:         50      16    1948     566

  Fragments arranged by size: 

                1948     566      50      16
 
PsiI TTA'TAA

Cuts at:      0    2138    2416    2580 
   Size:       2138     278     164

  Fragments arranged by size: 

                2138     278     164
 
PstI C_TGCA'G

Cuts at:      0     763    1668    2303    2580 
   Size:        763     905     635     277

  Fragments arranged by size: 

                 905     763     635     277
 
PvuII CAG'CTG

Cuts at:      0    1213    2580 
   Size:       1213    1367
 
SacI G_AGCT'C

Cuts at:      0    1579    2580 
   Size:       1579    1001
 
SalI G'TCGA_C

Cuts at:      0     569    2580 
   Size:        569    2011
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1955    2580 
   Size:       1955     625
 
SexAI A'CCwGG_T

Cuts at:      0    2016    2580 
   Size:       2016     564
 
SfcI C'TryA_G

Cuts at:      0     683     759    1440    1664    2299    2580 
   Size:        683      76     681     224     635     281

  Fragments arranged by size: 

                 683     681     635     281     224      76
 
SspI AAT'ATT

Cuts at:      0     359    2580 
   Size:        359    2221
 
StyI C'CwwG_G

Cuts at:      0    1814    2580 
   Size:       1814     766
 
TatI w'GTAC_w

Cuts at:      0     313    2580 
   Size:        313    2267
 
Tth111I GACn'n_nGTC

Cuts at:      0    1981    2580 
   Size:       1981     599
 
XbaI T'CTAG_A

Cuts at:      0    1516    2580 
   Size:       1516    1064
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      29    2580 
   Size:         29    2551
 
XmnI GAAnn'nnTTC

Cuts at:      0    1220    2580 
   Size:       1220    1360
 

 Enzymes that do cut:

     AccI     AclI     AcuI   AflIII     AgeI    AlwNI     ApoI     AvaI
     BanI    BanII    BciVI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI   BstEII    BstXI    BstYI    BtgZI     BtsI    CspCI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     PacI    PflMI     PfoI     PpiI    PpuMI
     PsiI     PstI    PvuII     SacI     SalI     SapI    SexAI     SfcI
     SspI     StyI     TatI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BseRI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI   BssHII    BstBI  BstZ17I   Bsu36I
     BtgI     ClaI     DraI   DraIII     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PasI     PciI
     PmeI     PmlI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TstI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com