Restriction map for pF1KB5633
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaHI       
BsgI        BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraII       
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII        875  TCCGGA               T^CCGGA
AlwNI          72  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          520  ATTAAT               AT^TAAT
AsnI          520  ATTAAT               AT^TAAT
Asp700I       889  GAAACTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         985  GAGCTC               G[AT]GC[AT]^C
AvaI         1153  CCCGGG               C^[CT]CG[GA]G
AvaII         873  GGTCC                G^G[AT]CC
BamHI          31  GGATCC               G^GATCC
BanII         985  GAGCTC               G[GA]GC[CT]^C
BclI         1305  TGATCA               T^GATCA
BfaI          697  CTAG                 C^TAG
BlpI         1198  GCTCAGC              GC^T[ACGT]AGC
BmyI          985  GAGCTC               G[AGT]GC[ACT]^C
BpmI          135  CTCCAG               CTGGAG(16/14)
Bpu1102I     1198  GCTCAGC              GC^T[ACGT]AGC
BsaI         1494  GGTCTC               GGTCTC(1/5)
BsaWI         875  TCCGGA               [AT]^CCGG[AT]
BseAI         875  TCCGGA               T^CCGGA
BsiHKAI       985  GAGCTC               G[AT]GC[AT]^C
BsoBI        1153  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      985  GAGCTC               G[AGT]GC[ACT]^C
BspEI         875  TCCGGA               T^CCGGA
BspMI         301  GCAGGT               ACCTGC(4/8)
BsrDI        1046  GCAATG               GCAATG(2/0)
BstAPI        269  GCAAAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1001  TTCGAA               TT^CGAA
CelII        1198  GCTCAGC              GC^T[ACGT]AGC
DraIII        458  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
FauI         1502  CCCGC                CCCGC(4/6)
FbaI         1305  TGATCA               T^GATCA
HgaI          498  GCGTC                GACGC(5/10)
HincII        319  GTTGAC               GT[CT]^[GA]AC
HindII        319  GTTGAC               GT[CT]^[GA]AC
MaeI          697  CTAG                 C^TAG
MaeII         372  ACGT                 A^CGT
MroI          875  TCCGGA               T^CCGGA
MspA1I         24  CAGCTG               C[AC]G^C[GT]G
NcoI           65  CCATGG               C^CATGG
NspI         1169  GCATGC               [GA]CATG^[CT]
NspV         1001  TTCGAA               TT^CGAA
PshBI         520  ATTAAT               AT^TAAT
PvuII          24  CAGCTG               CAG^CTG
SacI          985  GAGCTC               GAGCT^C
SfuI         1001  TTCGAA               TT^CGAA
SmaI         1153  CCCGGG               CCC^GGG
SphI         1169  GCATGC               GCATG^C
SspI          523  AATATT               AAT^ATT
XbaI          696  TCTAGA               T^CTAGA
XmaCI        1153  CCCGGG               C^CCGGG
XmaI         1153  CCCGGG               C^CCGGG
XmnI          889  GAAACTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI         1503  CCGC                 CCGC(-3/-1)
              254  GCGG                
Aor51HI        82  AGCGCT               AGC^GCT
             1202  AGCGCT              
BalI           62  TGGCCA               TGG^CCA
               68  TGGCCA              
BbvI          577  GCTGC                GCAGC(8/12)
             1365  GCTGC               
BseRI         151  GAGGAG               GAGGAG(10/8)
             1105  GAGGAG              
BsmFI         485  GTCCC                GGGAC(10/14)
              500  GTCCC               
BsmI          205  GAATGC               GAATGC(1/-1)
             1082  GAATGC              
DsaI           65  CCATGG               C^C[GA][CT]GG
             1393  CCACGG              
EarI         1102  GAAGAG               CTCTTC(1/4)
             1339  GAAGAG              
Eco47III       82  AGCGCT               AGC^GCT
             1202  AGCGCT              
EcoT22I      1084  ATGCAT               ATGCA^T
             1429  ATGCAT              
HaeII          82  AGCGCT               [GA]GCGC^[CT]
             1202  AGCGCT              
HphI          393  GGTGA                GGTGA(8/7)
              897  TCACC               
Ksp632I      1102  GAAGAG               CTCTTC(1/4)
             1339  GAAGAG              
MluNI          62  TGGCCA               TGG^CCA
               68  TGGCCA              
MscI           62  TGGCCA               TGG^CCA
               68  TGGCCA              
MseI          521  TTAA                 T^TAA
             1463  TTAA                
MslI          807  CATGAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1037  CACAGCAGTG          
NsiI         1084  ATGCAT               ATGCA^T
             1429  ATGCAT              
PleI          853  GACTC                GAGTC(4/5)
                3  GAGTC               
ScaI          733  AGTACT               AGT^ACT
             1549  AGTACT              
SfcI           78  CTGTAG               C^T[GA][CT]AG
              707  CTACAG              
SmlI          442  CTTGAG               C^T[CT][GA]AG
             1009  CTTGAG              
TaqI         1002  TCGA                 T^CGA
             1031  TCGA                
Tru9I         521  TTAA                 T^TAA
             1463  TTAA                
TseI          577  GCTGC                G^C[AT]GC
             1365  GCTGC               
TthHB8I      1002  TCGA                 T^CGA
             1031  TCGA                
XcmI          137  CCAGCTCATTTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              212  CCATCTGCTTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        Tsp45I      Tsp509I     TspRI       XhoII       
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