Restriction map for pF1KB5631
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        Asp700I     AspEI       AspI        AviII       
AvrII       BalI        BbeI        BbrPI       BciVI       
BclI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaI        BseAI       BsiEI       
BsiWI       Bsp1407I    BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       CpoI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoT22I     
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HpaI        KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        Tth111I     
XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        288  GGTACC               G^GTACC
AccII          89  CGCG                 CG^CG
AflII        1566  CTTAAG               C^TTAAG
Aor51HI      1982  AGCGCT               AGC^GCT
AseI          298  ATTAAT               AT^TAAT
AsnI          298  ATTAAT               AT^TAAT
Asp718I       288  GGTACC               G^GTACC
BbvCI        2983  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2280  CGATGCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         1566  CTTAAG               C^TTAAG
BglII         685  AGATCT               A^GATCT
BpmI          689  CTGGAG               CTGGAG(16/14)
BsaBI        1125  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2891  TCCGGT               [AT]^CCGG[AT]
BsmBI        1520  CGTCTC               CGTCTC(1/5)
BspDI        1013  ATCGAT               AT^CGAT
BsrBI        2121  CCGCTC               CCGCTC(-3/-3)
BssSI        1955  CTCGTG               CACGAG(-5/-1)
BstAPI       1704  GCACCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2365  TTCGAA               TT^CGAA
BstUI          89  CGCG                 CG^CG
BstXI         981  CCAGAAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1983  GCGC                 GCG^C
ClaI         1013  ATCGAT               AT^CGAT
EaeI         2730  TGGCCG               [CT]^GGCC[GA]
Eco47III     1982  AGCGCT               AGC^GCT
EcoNI        1448  CCTATGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        2078  GATATC               GAT^ATC
HaeII        1982  AGCGCT               [GA]GCGC^[CT]
HhaI         1983  GCGC                 GCG^C
HinP1I       1983  GCGC                 G^CGC
HindIII      1396  AAGCTT               A^AGCTT
KpnI          288  GGTACC               GGTAC^C
MamI         1125  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI           89  CGCG                 CG^CG
NcoI         3092  CCATGG               C^CATGG
NdeI         1025  CATATG               CA^TATG
NspV         2365  TTCGAA               TT^CGAA
PaeR7I        470  CTCGAG               C^TCGAG
PshBI         298  ATTAAT               AT^TAAT
SacI         2533  GAGCTC               GAGCT^C
SapI          731  GAAGAGC              GCTCTTC(1/4)
SfuI         2365  TTCGAA               TT^CGAA
SphI         2639  GCATGC               GCATG^C
SwaI          333  ATTTAAAT             ATTT^AAAT
XcmI         3083  CCATAGTGCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          470  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII        128  ACGTGT               A^C[GA][CT]GT
              437  ACACGT              
AlwNI        1809  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2687  CAGAGACTG           
AspHI        1471  GAGCAC               G[AT]GC[AT]^C
             2533  GAGCTC              
AvaI          470  CTCGAG               C^[CT]CG[GA]G
             2741  CCCGAG              
BamHI        2297  GGATCC               G^GATCC
             2811  GGATCC              
BanII        1382  GGGCTC               G[GA]GC[CT]^C
             2533  GAGCTC              
BbsI          431  GAAGAC               GAAGAC(2/6)
             1843  GTCTTC              
BfaI          428  CTAG                 C^TAG
             2548  CTAG                
BpuAI         431  GAAGAC               GAAGAC(2/6)
             1843  GTCTTC              
BsiHKAI      1471  GAGCAC               G[AT]GC[AT]^C
             2533  GAGCTC              
BsmI         1044  GAATGC               GAATGC(1/-1)
             1208  GCATTC              
BsoBI         470  CTCGAG               C^[CT]CG[GA]G
             2741  CCCGAG              
BspMI        1539  ACCTGC               ACCTGC(4/8)
             1709  ACCTGC              
BsrFI        2383  ACCGGC               [GA]^CCGG[CT]
             2732  GCCGGT              
Cfr10I       2383  ACCGGC               [GA]^CCGG[CT]
             2732  GCCGGT              
DraI          334  TTTAAA               TTT^AAA
              921  TTTAAA              
EcoRI         841  GAATTC               G^AATTC
              973  GAATTC              
FauI           90  GCGGG                CCCGC(4/6)
             1503  GCGGG               
HgaI         1717  GACGC                GACGC(5/10)
             1317  GCGTC               
HphI         1338  GGTGA                GGTGA(8/7)
             1386  TCACC               
MaeI          428  CTAG                 C^TAG
             2548  CTAG                
MslI         1332  CATAAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1482  CACAGACGTG          
PstI           40  CTGCAG               CTGCA^G
             1934  CTGCAG              
PvuII         628  CAGCTG               CAG^CTG
             2929  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BanI        BbvI        
BcnI        BmyI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       
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