Restriction map for pF1KB5613
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BanII       
BbeI        BbrPI       BbsI        BciVI       BclI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
FauI        FbaI        FseI        FspI        HindIII     
KasI        KpnI        KspI        MaeI        MaeII       
MfeI        MluI        MroI        MslI        MunI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   71 enzymes) #
###################################

AccII         698  CGCG                 CG^CG
AcyI          667  GACGCC               G[GA]^CG[CT]C
AocI          196  CCTAAGG              CC^T[ACGT]AGG
AseI          874  ATTAAT               AT^TAAT
AsnI          874  ATTAAT               AT^TAAT
Asp700I       680  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         205  GTGCTC               G[AT]GC[AT]^C
AvaI         1037  CTCGAG               C^[CT]CG[GA]G
BalI          821  TGGCCA               TGG^CCA
BamHI         566  GGATCC               G^GATCC
BbvCI         880  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1231  CGATACAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          563  CCCGG                CC^[GC]GG
BglI           93  GCCACCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          205  GTGCTC               G[AGT]GC[ACT]^C
BsaBI        1246  GATATGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         667  GACGCC               G[GA]^CG[CT]C
BsaI         1148  GAGACC               GGTCTC(1/5)
BsgI          112  GTGCAG               GTGCAG(16/14)
BsiHKAI       205  GTGCTC               G[AT]GC[AT]^C
BsmBI        1034  CGTCTC               CGTCTC(1/5)
BsoBI        1037  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      205  GTGCTC               G[AGT]GC[ACT]^C
BspMI          44  ACCTGC               ACCTGC(4/8)
BsrBI         925  GAGCGG               CCGCTC(-3/-3)
BstAPI        409  GCAGCTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1278  CATCC                GGATG(2/0)
BstUI         698  CGCG                 CG^CG
BstYI         566  GGATCC               [GA]^GATC[CT]
Bsu36I        196  CCTAAGG              CC^T[ACGT]AGG
CfoI          560  GCGC                 GCG^C
DraI          661  TTTAAA               TTT^AAA
DraII         260  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          818  CCATGG               C^C[GA][CT]GG
EaeI          821  TGGCCA               [CT]^GGCC[GA]
Eco81I        196  CCTAAGG              CC^T[ACGT]AGG
EcoO109I      260  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         423  GATATC               GAT^ATC
EcoT14I       818  CCATGG               C^C[AT][AT]GG
FokI         1278  CATCC                GGATG(9/13)
HaeII         559  AGCGCC               [GA]GCGC^[CT]
HapII         564  CCGG                 C^CGG
HhaI          560  GCGC                 GCG^C
Hin1I         667  GACGCC               G[GA]^CG[CT]C
HinP1I        560  GCGC                 G^CGC
HincII         58  GTTAAC               GT[CT]^[GA]AC
HindII         58  GTTAAC               GT[CT]^[GA]AC
HpaI           58  GTTAAC               GTT^AAC
HpaII         564  CCGG                 C^CGG
MamI         1246  GATATGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          566  GGATCC               [GA]^GATC[CT]
MluNI         821  TGGCCA               TGG^CCA
MscI          821  TGGCCA               TGG^CCA
MspI          564  CCGG                 C^CGG
MvnI          698  CGCG                 CG^CG
NciI          563  CCCGG                CC^[GC]GG
NcoI          818  CCATGG               C^CATGG
NdeI          734  CATATG               CA^TATG
PaeR7I       1037  CTCGAG               C^TCGAG
PpuMI         260  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1374  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         874  ATTAAT               AT^TAAT
SapI         1137  GCTCTTC              GCTCTTC(1/4)
SbfI           45  CCTGCAGG             CCTGCA^GG
SfaNI        1251  GCATC                GCATC(5/9)
SmlI         1037  CTCGAG               C^T[CT][GA]AG
Sse8387I       45  CCTGCAGG             CCTGCA^GG
StyI          818  CCATGG               C^C[AT][AT]GG
XhoI         1037  CTCGAG               C^TCGAG
XhoII         566  GGATCC               [GA]^GATC[CT]
XmnI          680  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI          939  AAATTT               [GA]^AATT[CT]
             1167  GAATTT              
AlwI          567  GATCC                GGATC(4/5)
              566  GGATC               
AlwNI         410  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1208  CAGTTGCTG           
ApoI          939  AAATTT               [GA]^AATT[CT]
             1167  GAATTT              
BanI          163  GGCACC               G^G[CT][GA]CC
             1266  GGTGCC              
BstEII         40  GGTTACC              G^GT[ACGT]ACC
             1045  GGTCACC             
BstPI          40  GGTTACC              G^GT[ACGT]ACC
             1045  GGTCACC             
EcoO65I        40  GGTTACC              G^GT[ACGT]ACC
             1045  GGTCACC             
EcoT22I       731  ATGCAT               ATGCA^T
             1249  ATGCAT              
HaeIII        822  GGCC                 GG^CC
              858  GGCC                
HphI          830  TCACC                GGTGA(8/7)
             1047  TCACC               
NsiI          731  ATGCAT               ATGCA^T
             1249  ATGCAT              
NspI          230  GCATGT               [GA]CATG^[CT]
             1300  ACATGC              
PstI           46  CTGCAG               CTGCA^G
              407  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsrI        BssKI       BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      HgaI        HinfI       ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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