Restriction map for pF1KB5613
 (Linear) (Six-base) MAPSORT of: KIBB5613.seq  Check: 2537  from: 1  to: 1503



KIBB5613.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     788    1503 
   Size:        139     649     715

  Fragments arranged by size: 

                 715     649     139
 
AlwNI CAG_nnn'CTG

Cuts at:      0     415    1213    1503 
   Size:        415     798     290

  Fragments arranged by size: 

                 798     415     290
 
ApoI r'AATT_y

Cuts at:      0     939    1167    1503 
   Size:        939     228     336

  Fragments arranged by size: 

                 939     336     228
 
AseI AT'TA_AT

Cuts at:      0     875    1503 
   Size:        875     628
 
AvaI C'yCGr_G

Cuts at:      0    1037    1503 
   Size:       1037     466
 
BamHI G'GATC_C

Cuts at:      0     566    1503 
   Size:        566     937
 
BanI G'GyrC_C

Cuts at:      0     163    1266    1503 
   Size:        163    1103     237

  Fragments arranged by size: 

                1103     237     163
 
BbvCI CC'TCA_GC

Cuts at:      0     881    1503 
   Size:        881     622
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1220    1254    1503 
   Size:       1220      34     249

  Fragments arranged by size: 

                1220     249      34
 
BfrBI ATG'CAT

Cuts at:      0     733    1251    1503 
   Size:        733     518     252

  Fragments arranged by size: 

                 733     518     252
 
BglI GCCn_nnn'nGGC

Cuts at:      0      99    1503 
   Size:         99    1404
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     943    1503 
   Size:        943     560
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     341     576     644     999    1166    1397    1503 
   Size:        193     148     235      68     355     167     231     106

  Fragments arranged by size: 

                 355     235     231     193     167     148     106      68
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     881    1503 
   Size:        881     622
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1142    1503 
   Size:       1142     361
 
BsaBI GATnn'nnATC

Cuts at:      0    1250    1503 
   Size:       1250     253
 
BsaHI Gr'CG_yC

Cuts at:      0     668    1503 
   Size:        668     835
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     382     898    1082    1503 
   Size:        382     516     184     421

  Fragments arranged by size: 

                 516     421     382     184
 
BseYI C'CCAG_C

Cuts at:      0    1213    1281    1503 
   Size:       1213      68     222

  Fragments arranged by size: 

                1213     222      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1503 
   Size:        133    1370
 
BsiHKAI G_wGCw'C

Cuts at:      0     209    1503 
   Size:        209    1294
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1040    1503 
   Size:       1040     463
 
Bsp1286I G_dGCh'C

Cuts at:      0     209    1503 
   Size:        209    1294
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      53    1503 
   Size:         53    1450
 
BsrBI CCG'CTC

Cuts at:      0     927    1503 
   Size:        927     576
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     415    1503 
   Size:        415    1088
 
BstEII G'GTnAC_C

Cuts at:      0      40    1045    1503 
   Size:         40    1005     458

  Fragments arranged by size: 

                1005     458      40
 
BstYI r'GATC_y

Cuts at:      0     566    1503 
   Size:        566     937
 
Bsu36I CC'TnA_GG

Cuts at:      0     197    1503 
   Size:        197    1306
 
BtgI C'CryG_G

Cuts at:      0     818    1503 
   Size:        818     685
 
BtsI GCAGTG_nn'

Cuts at:      0     911     917    1503 
   Size:        911       6     586

  Fragments arranged by size: 

                 911     586       6
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     887     922    1503 
   Size:        887      35     581

  Fragments arranged by size: 

                 887     581      35
 
DraI TTT'AAA

Cuts at:      0     663    1503 
   Size:        663     840
 
EaeI y'GGCC_r

Cuts at:      0     821    1503 
   Size:        821     682
 
EarI CTCTTCn'nnn_

Cuts at:      0     115     675    1144    1312    1503 
   Size:        115     560     469     168     191

  Fragments arranged by size: 

                 560     469     191     168     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     193     341     576     644     788     999    1166 
   Size:        139      54     148     235      68     144     211     167

Cuts at:   1166    1397    1503 
   Size:        231     106

  Fragments arranged by size: 

                 235     231     211     167     148     144     139     106
                  68      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     261    1503 
   Size:        261    1242
 
EcoRV GAT'ATC

Cuts at:      0     425    1503 
   Size:        425    1078
 
HaeII r_GCGC'y

Cuts at:      0     563    1503 
   Size:        563     940
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     167     199    1503 
   Size:        167      32    1304

  Fragments arranged by size: 

                1304     167      32
 
HincII GTy'rAC

Cuts at:      0      60    1503 
   Size:         60    1443
 
HpaI GTT'AAC

Cuts at:      0      60    1503 
   Size:         60    1443
 
MscI TGG'CCA

Cuts at:      0     823    1503 
   Size:        823     680
 
MspA1I CmG'CkG

Cuts at:      0     117     508     554    1102    1503 
   Size:        117     391      46     548     401

  Fragments arranged by size: 

                 548     401     391     117      46
 
NcoI C'CATG_G

Cuts at:      0     818    1503 
   Size:        818     685
 
NdeI CA'TA_TG

Cuts at:      0     735    1503 
   Size:        735     768
 
NsiI A_TGCA'T

Cuts at:      0     735    1253    1503 
   Size:        735     518     250

  Fragments arranged by size: 

                 735     518     250
 
NspI r_CATG'y

Cuts at:      0     234    1304    1503 
   Size:        234    1070     199

  Fragments arranged by size: 

                1070     234     199
 
PasI CC'CwG_GG

Cuts at:      0      78    1503 
   Size:         78    1425
 
PpuMI rG'GwC_Cy

Cuts at:      0     261    1503 
   Size:        261    1242
 
PshAI GACnn'nnGTC

Cuts at:      0    1378    1503 
   Size:       1378     125
 
PstI C_TGCA'G

Cuts at:      0      50     411    1503 
   Size:         50     361    1092

  Fragments arranged by size: 

                1092     361      50
 
PvuII CAG'CTG

Cuts at:      0     117     508     554    1102    1503 
   Size:        117     391      46     548     401

  Fragments arranged by size: 

                 548     401     391     117      46
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1144    1503 
   Size:       1144     359
 
SbfI CC_TGCA'GG

Cuts at:      0      50    1503 
   Size:         50    1453
 
SfcI C'TryA_G

Cuts at:      0      46     407     521    1503 
   Size:         46     361     114     982

  Fragments arranged by size: 

                 982     361     114      46
 
SmlI C'TyrA_G

Cuts at:      0    1037    1503 
   Size:       1037     466
 
StyI C'CwwG_G

Cuts at:      0     818    1503 
   Size:        818     685
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     372    1503 
   Size:        372    1131
 
TatI w'GTAC_w

Cuts at:      0    1345    1503 
   Size:       1345     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     240     272    1262    1294    1503 
   Size:        240      32     990      32     209

  Fragments arranged by size: 

                 990     240     209      32      32
 
XhoI C'TCGA_G

Cuts at:      0    1037    1503 
   Size:       1037     466
 
XmnI GAAnn'nnTTC

Cuts at:      0     684    1503 
   Size:        684     819
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI     AvaI    BamHI     BanI    BbvCI
     BcgI    BfrBI     BglI     BmrI     BpmI   Bpu10I     BsaI    BsaBI
    BsaHI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI   BstAPI   BstEII    BstYI   Bsu36I     BtgI     BtsI    CspCI
     DraI     EaeI     EarI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I
   HincII     HpaI     MscI   MspA1I     NcoI     NdeI     NsiI     NspI
     PasI    PpuMI    PshAI     PstI    PvuII     SapI     SbfI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI    AvrII     BaeI    BanII     BbeI     BbsI    BciVI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaAI
    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI  BstZ17I    BtgZI
     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
     FalI     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XmaI     ZraI
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