Restriction map for pF1KB5591
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BbrPI       BbsI        BbvCI       BcgI        
BclI        BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaI        BsiWI       BspDI       BspHI       
BssHII      BssSI       CelII       ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    EcoNI       
EcoRV       EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        MluI        
NdeI        NheI        NruI        NsiI        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SalI        SbfI        SfiI        
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Tth111I     XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         1717  GTATAC               GT^[AC][GT]AC
AflII        2731  CTTAAG               C^TTAAG
AflIII        660  ACATGT               A^C[GA][CT]GT
Asp700I      1606  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        1562  TGCGCA               TGC^GCA
BanI         2527  GGCGCC               G^G[CT][GA]CC
BbeI         2527  GGCGCC               GGCGC^C
BciVI         504  GTATCC               GTATCC(6/5)
BfaI          814  CTAG                 C^TAG
BfrI         2731  CTTAAG               C^TTAAG
BsaBI         552  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1804  GAGACG               CGTCTC(1/5)
BsmI          653  GCATTC               GAATGC(1/-1)
Bsp1407I     2561  TGTACA               T^GTACA
BspLU11I      660  ACATGT               A^CATGT
BsrBI        1410  CCGCTC               CCGCTC(-3/-3)
BsrGI        2561  TGTACA               T^GTACA
Bst1107I     1717  GTATAC               GTA^TAC
BstAPI       2190  GCAGGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1645  TTCGAA               TT^CGAA
BstEII        130  GGTTACC              G^GT[ACGT]ACC
BstPI         130  GGTTACC              G^GT[ACGT]ACC
BstXI        1304  CCAACATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1717  GTATAC               GTA^TAC
EagI         2172  CGGCCG               C^GGCCG
EclXI        2172  CGGCCG               C^GGCCG
Eco52I       2172  CGGCCG               C^GGCCG
EcoO65I       130  GGTTACC              G^GT[ACGT]ACC
EcoRI          96  GAATTC               G^AATTC
FspI         1562  TGCGCA               TGC^GCA
KasI         2527  GGCGCC               G^GCGCC
MaeI          814  CTAG                 C^TAG
MamI          552  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1932  CAATTG               C^AATTG
MunI         1932  CAATTG               C^AATTG
NarI         2527  GGCGCC               GG^CGCC
NcoI          933  CCATGG               C^CATGG
NotI         2171  GCGGCCGC             GC^GGCCGC
NspV         1645  TTCGAA               TT^CGAA
PaeR7I       1951  CTCGAG               C^TCGAG
PflMI         658  CCACATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI          683  AGTACT               AGT^ACT
SexAI         794  ACCTGGT              A^CC[AT]GGT
SfoI         2527  GGCGCC               GGC^GCC
SfuI         1645  TTCGAA               TT^CGAA
SgrAI        1736  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        2561  TGTACA               T^GTACA
Van91I        658  CCACATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1951  CTCGAG               C^TCGAG
XmnI         1606  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccIII       1994  TCCGGA               T^CCGGA
             2341  TCCGGA              
AcsI           96  GAATTC               [GA]^AATT[CT]
             2052  GAATTT              
AcyI         2527  GGCGCC               G[GA]^CG[CT]C
             2611  GGCGTC              
AocI         1435  CCTCAGG              CC^T[ACGT]AGG
             2516  CCTCAGG             
ApoI           96  GAATTC               [GA]^AATT[CT]
             2052  GAATTT              
BalI          539  TGGCCA               TGG^CCA
              764  TGGCCA              
BamHI         728  GGATCC               G^GATCC
             2571  GGATCC              
BglI         1409  GCCGCTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1586  GCCTAAACGGC         
BsaHI        2527  GGCGCC               G[GA]^CG[CT]C
             2611  GGCGTC              
BseAI        1994  TCCGGA               T^CCGGA
             2341  TCCGGA              
BspEI        1994  TCCGGA               T^CCGGA
             2341  TCCGGA              
BsrDI        1332  CATTGC               GCAATG(2/0)
              830  GCAATG              
Bsu36I       1435  CCTCAGG              CC^T[ACGT]AGG
             2516  CCTCAGG             
DraIII         52  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1869  CACAGAGTG           
Eco81I       1435  CCTCAGG              CC^T[ACGT]AGG
             2516  CCTCAGG             
EcoT14I       767  CCAAGG               C^C[AT][AT]GG
              933  CCATGG              
HaeII        2184  GGCGCT               [GA]GCGC^[CT]
             2527  GGCGCC              
HgaI         1806  GACGC                GACGC(5/10)
             2612  GCGTC               
Hin1I        2527  GGCGCC               G[GA]^CG[CT]C
             2611  GGCGTC              
KspI         1518  CCGCGG               CCGC^GG
             2175  CCGCGG              
MaeII        2035  ACGT                 A^CGT
             2337  ACGT                
MluNI         539  TGGCCA               TGG^CCA
              764  TGGCCA              
MroI         1994  TCCGGA               T^CCGGA
             2341  TCCGGA              
MscI          539  TGGCCA               TGG^CCA
              764  TGGCCA              
MseI         1006  TTAA                 T^TAA
             2732  TTAA                
MspA1I       1518  CCGCGG               C[AC]G^C[GT]G
             2175  CCGCGG              
NaeI          926  GCCGGC               GCC^GGC
             1737  GCCGGC              
NgoMIV        926  GCCGGC               G^CCGGC
             1737  GCCGGC              
SacII        1518  CCGCGG               CCGC^GG
             2175  CCGCGG              
SapI         1824  GAAGAGC              GCTCTTC(1/4)
             1269  GCTCTTC             
SmlI         1951  CTCGAG               C^T[CT][GA]AG
             2731  CTTAAG              
StuI         1326  AGGCCT               AGG^CCT
             2637  AGGCCT              
StyI          767  CCAAGG               C^C[AT][AT]GG
              933  CCATGG              
Tru9I        1006  TTAA                 T^TAA
             2732  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BglII       BmyI        BpmI        
BsaJI       BsaWI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       
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