Restriction map for pF1KB5591
 (Linear) (Six-base) MAPSORT of: KIBB5591.seq  Check: 3031  from: 1  to: 2778



KIBB5591.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     594    2132    2181    2778 
   Size:        594    1538      49     597

  Fragments arranged by size: 

                1538     597     594      49
 
AccI GT'mk_AC

Cuts at:      0    1718    2778 
   Size:       1718    1060
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    1447    2778 
   Size:        458     989    1331

  Fragments arranged by size: 

                1331     989     458
 
AflII C'TTAA_G

Cuts at:      0    2731    2778 
   Size:       2731      47
 
AflIII A'CryG_T

Cuts at:      0     660    2778 
   Size:        660    2118
 
AleI CACnn'nnGTG

Cuts at:      0    1540    2778 
   Size:       1540    1238
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2322    2354    2778 
   Size:       2322      32     424

  Fragments arranged by size: 

                2322     424      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     436     573    2524    2778 
   Size:        436     137    1951     254

  Fragments arranged by size: 

                1951     436     254     137
 
ApoI r'AATT_y

Cuts at:      0      96    2052    2778 
   Size:         96    1956     726

  Fragments arranged by size: 

                1956     726      96
 
AvaI C'yCGr_G

Cuts at:      0     345    1413    1951    2667    2778 
   Size:        345    1068     538     716     111

  Fragments arranged by size: 

                1068     716     538     345     111
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1233    1266    2778 
   Size:       1233      33    1512

  Fragments arranged by size: 

                1512    1233      33
 
BamHI G'GATC_C

Cuts at:      0     728    2571    2778 
   Size:        728    1843     207

  Fragments arranged by size: 

                1843     728     207
 
BanI G'GyrC_C

Cuts at:      0    2527    2778 
   Size:       2527     251
 
BanII G_rGCy'C

Cuts at:      0     352    1271    1384    1518    1961    2605    2680    2720 
   Size:        352     919     113     134     443     644      75      40

Cuts at:   2720    2778 
   Size:         58

  Fragments arranged by size: 

                 919     644     443     352     134     113      75      58
                  40
 
BbeI G_GCGC'C

Cuts at:      0    2531    2778 
   Size:       2531     247
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     515    2778 
   Size:        515    2263
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1415    1592    2778 
   Size:       1415     177    1186

  Fragments arranged by size: 

                1415    1186     177
 
BglII A'GATC_T

Cuts at:      0     419    1346    1470    2778 
   Size:        419     927     124    1308

  Fragments arranged by size: 

                1308     927     419     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1379    1611    2617    2778 
   Size:       1379     232    1006     161

  Fragments arranged by size: 

                1379    1006     232     161
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607     970    1285    1398    1780    2001    2410    2514 
   Size:        607     363     315     113     382     221     409     104

Cuts at:   2514    2719    2778 
   Size:        205      59

  Fragments arranged by size: 

                 607     409     382     363     315     221     205     113
                 104      59
 
Bpu10I CC'TnA_GC

Cuts at:      0    2674    2778 
   Size:       2674     104
 
BsaBI GATnn'nnATC

Cuts at:      0     556    2778 
   Size:        556    2222
 
BsaHI Gr'CG_yC

Cuts at:      0    2528    2612    2778 
   Size:       2528      84     166

  Fragments arranged by size: 

                2528     166      84
 
BsaWI w'CCGG_w

Cuts at:      0    1994    2030    2341    2778 
   Size:       1994      36     311     437

  Fragments arranged by size: 

                1994     437     311      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     133     163    1278    1308    1371    1401    1717    1747 
   Size:        133      30    1115      30      63      30     316      30

Cuts at:   1747    2140    2170    2206    2236    2778 
   Size:        393      30      36      30     542

  Fragments arranged by size: 

                1115     542     393     316     133      63      36      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     333    1468    1538    1876    1969    2778 
   Size:        333    1135      70     338      93     809

  Fragments arranged by size: 

                1135     809     338     333      93      70
 
BseYI C'CCAG_C

Cuts at:      0    1311    1442    1628    1767    1809    2284    2778 
   Size:       1311     131     186     139      42     475     494

  Fragments arranged by size: 

                1311     494     475     186     139     131      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507    1409    2637    2654    2673    2778 
   Size:        507     902    1228      17      19     105

  Fragments arranged by size: 

                1228     902     507     105      19      17
 
BsiEI CG_ry'CG

Cuts at:      0    2031    2175    2540    2778 
   Size:       2031     144     365     238

  Fragments arranged by size: 

                2031     365     238     144
 
BsiHKAI G_wGCw'C

Cuts at:      0    1271    2605    2680    2720    2778 
   Size:       1271    1334      75      40      58

  Fragments arranged by size: 

                1334    1271      75      58      40
 
BsmI GAATG_Cn'

Cuts at:      0     653    2778 
   Size:        653    2125
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1798    2778 
   Size:       1798     980
 
Bsp1286I G_dGCh'C

Cuts at:      0     352    1271    1384    1518    1961    2605    2680    2720 
   Size:        352     919     113     134     443     644      75      40

Cuts at:   2720    2778 
   Size:         58

  Fragments arranged by size: 

                 919     644     443     352     134     113      75      58
                  40
 
BspEI T'CCGG_A

Cuts at:      0    1994    2341    2778 
   Size:       1994     347     437

  Fragments arranged by size: 

                1994     437     347
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     594    1004    2132    2181    2778 
   Size:        594     410    1128      49     597

  Fragments arranged by size: 

                1128     597     594     410      49
 
BsrBI CCG'CTC

Cuts at:      0    1412    2778 
   Size:       1412    1366
 
BsrDI GCAATG_nn'

Cuts at:      0     837    1331    2778 
   Size:        837     494    1447

  Fragments arranged by size: 

                1447     837     494
 
BsrFI r'CCGG_y

Cuts at:      0     926    1124    1737    2778 
   Size:        926     198     613    1041

  Fragments arranged by size: 

                1041     926     613     198
 
BsrGI T'GTAC_A

Cuts at:      0    2561    2778 
   Size:       2561     217
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2196    2778 
   Size:       2196     582
 
BstBI TT'CG_AA

Cuts at:      0    1646    2778 
   Size:       1646    1132
 
BstEII G'GTnAC_C

Cuts at:      0     130    2778 
   Size:        130    2648
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1311    2778 
   Size:       1311    1467
 
BstYI r'GATC_y

Cuts at:      0     419     728    1346    1470    2571    2778 
   Size:        419     309     618     124    1101     207

  Fragments arranged by size: 

                1101     618     419     309     207     124
 
BstZ17I GTA'TAC

Cuts at:      0    1719    2778 
   Size:       1719    1059
 
Bsu36I CC'TnA_GG

Cuts at:      0    1436    2517    2778 
   Size:       1436    1081     261

  Fragments arranged by size: 

                1436    1081     261
 
BtgI C'CryG_G

Cuts at:      0     933    1518    1796    2165    2175    2778 
   Size:        933     585     278     369      10     603

  Fragments arranged by size: 

                 933     603     585     369     278      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     306    1008    2577    2778 
   Size:        306     702    1569     201

  Fragments arranged by size: 

                1569     702     306     201
 
BtsI GCAGTG_nn'

Cuts at:      0    1201    1535    2429    2778 
   Size:       1201     334     894     349

  Fragments arranged by size: 

                1201     894     349     334
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     294     329     497     532     669     704    1157    1192 
   Size:        294      35     168      35     137      35     453      35

Cuts at:   1192    2778 
   Size:       1586

  Fragments arranged by size: 

                1586     453     294     168     137      35      35      35
                  35
 
DraIII CAC_nnn'GTG

Cuts at:      0      57    1874    2778 
   Size:         57    1817     904

  Fragments arranged by size: 

                1817     904      57
 
EaeI y'GGCC_r

Cuts at:      0     539     764     855    1019    2172    2778 
   Size:        539     225      91     164    1153     606

  Fragments arranged by size: 

                1153     606     539     225     164      91
 
EagI C'GGCC_G

Cuts at:      0    2172    2778 
   Size:       2172     606
 
EarI CTCTTCn'nnn_

Cuts at:      0    1276    1362    1819    1826    1853    2778 
   Size:       1276      86     457       7      27     925

  Fragments arranged by size: 

                1276     925     457      86      27       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1216    1341    2286    2778 
   Size:       1216     125     945     492

  Fragments arranged by size: 

                1216     945     492     125
 
EcoICRI GAG'CTC

Cuts at:      0    1269    2603    2678    2718    2778 
   Size:       1269    1334      75      40      60

  Fragments arranged by size: 

                1334    1269      75      60      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     607     970    1285    1398    1447    1780    2001 
   Size:        458     149     363     315     113      49     333     221

Cuts at:   2001    2410    2514    2719    2778 
   Size:        409     104     205      59

  Fragments arranged by size: 

                 458     409     363     333     315     221     205     149
                 113     104      59      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1617    1625    1813    2778 
   Size:       1617       8     188     965

  Fragments arranged by size: 

                1617     965     188       8
 
EcoRI G'AATT_C

Cuts at:      0      96    2778 
   Size:         96    2682
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     273     792    2778 
   Size:        273     519    1986

  Fragments arranged by size: 

                1986     519     273
 
FspI TGC'GCA

Cuts at:      0    1564    2778 
   Size:       1564    1214
 
HaeII r_GCGC'y

Cuts at:      0    2188    2531    2778 
   Size:       2188     343     247

  Fragments arranged by size: 

                2188     343     247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     133     165     271     303     763     795    1517    1549 
   Size:        133      32     106      32     460      32     722      32

Cuts at:   1549    1717    1749    2778 
   Size:        168      32    1029

  Fragments arranged by size: 

                1029     722     460     168     133     106      32      32
                  32      32      32
 
KasI G'GCGC_C

Cuts at:      0    2527    2778 
   Size:       2527     251
 
MfeI C'AATT_G

Cuts at:      0    1932    2778 
   Size:       1932     846
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     995    1328    1948    2778 
   Size:        401     594     333     620     830

  Fragments arranged by size: 

                 830     620     594     401     333
 
MscI TGG'CCA

Cuts at:      0     541     766    2778 
   Size:        541     225    2012

  Fragments arranged by size: 

                2012     541     225
 
MslI CAynn'nnrTG

Cuts at:      0     754     760     938    1540    2596    2778 
   Size:        754       6     178     602    1056     182

  Fragments arranged by size: 

                1056     754     602     182     178       6
 
MspA1I CmG'CkG

Cuts at:      0    1520    2177    2778 
   Size:       1520     657     601

  Fragments arranged by size: 

                1520     657     601
 
NaeI GCC'GGC

Cuts at:      0     928    1739    2778 
   Size:        928     811    1039

  Fragments arranged by size: 

                1039     928     811
 
NarI GG'CG_CC

Cuts at:      0    2528    2778 
   Size:       2528     250
 
NcoI C'CATG_G

Cuts at:      0     933    2778 
   Size:        933    1845
 
NgoMIV G'CCGG_C

Cuts at:      0     926    1737    2778 
   Size:        926     811    1041

  Fragments arranged by size: 

                1041     926     811
 
NotI GC'GGCC_GC

Cuts at:      0    2172    2778 
   Size:       2172     606
 
NspI r_CATG'y

Cuts at:      0     664    1311    1659    2778 
   Size:        664     647     348    1119

  Fragments arranged by size: 

                1119     664     647     348
 
PciI A'CATG_T

Cuts at:      0     660    2778 
   Size:        660    2118
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     664    2778 
   Size:        664    2114
 
PpuMI rG'GwC_Cy

Cuts at:      0    1617    1625    1813    2778 
   Size:       1617       8     188     965

  Fragments arranged by size: 

                1617     965     188       8
 
PspXI vC'TCGA_Gb

Cuts at:      0    1951    2778 
   Size:       1951     827
 
PstI C_TGCA'G

Cuts at:      0     569    1891    2069    2192    2778 
   Size:        569    1322     178     123     586

  Fragments arranged by size: 

                1322     586     569     178     123
 
SacI G_AGCT'C

Cuts at:      0    1271    2605    2680    2720    2778 
   Size:       1271    1334      75      40      58

  Fragments arranged by size: 

                1334    1271      75      58      40
 
SacII CC_GC'GG

Cuts at:      0    1521    2178    2778 
   Size:       1521     657     600

  Fragments arranged by size: 

                1521     657     600
 
SanDI GG'GwC_CC

Cuts at:      0    1813    2778 
   Size:       1813     965
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1276    1819    2778 
   Size:       1276     543     959

  Fragments arranged by size: 

                1276     959     543
 
ScaI AGT'ACT

Cuts at:      0     685    2778 
   Size:        685    2093
 
SexAI A'CCwGG_T

Cuts at:      0     794    2778 
   Size:        794    1984
 
SfcI C'TryA_G

Cuts at:      0     565    1581    1887    2065    2188    2778 
   Size:        565    1016     306     178     123     590

  Fragments arranged by size: 

                1016     590     565     306     178     123
 
SfoI GGC'GCC

Cuts at:      0    2529    2778 
   Size:       2529     249
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1737    2778 
   Size:       1737    1041
 
SmlI C'TyrA_G

Cuts at:      0    1951    2731    2778 
   Size:       1951     780      47

  Fragments arranged by size: 

                1951     780      47
 
StuI AGG'CCT

Cuts at:      0    1328    2639    2778 
   Size:       1328    1311     139

  Fragments arranged by size: 

                1328    1311     139
 
StyI C'CwwG_G

Cuts at:      0     767     933    2778 
   Size:        767     166    1845

  Fragments arranged by size: 

                1845     767     166
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1378    1497    2554    2778 
   Size:       1378     119    1057     224

  Fragments arranged by size: 

                1378    1057     224     119
 
TatI w'GTAC_w

Cuts at:      0     683    2561    2778 
   Size:        683    1878     217

  Fragments arranged by size: 

                1878     683     217
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1278    1310    1371    1403    1805    1837    2556    2588 
   Size:       1278      32      61      32     402      32     719      32

Cuts at:   2588    2597    2629    2778 
   Size:          9      32     149

  Fragments arranged by size: 

                1278     719     402     149      61      32      32      32
                  32      32       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     549    2292    2604    2778 
   Size:        549    1743     312     174

  Fragments arranged by size: 

                1743     549     312     174
 
XhoI C'TCGA_G

Cuts at:      0    1951    2778 
   Size:       1951     827
 
XmnI GAAnn'nnTTC

Cuts at:      0    1610    2778 
   Size:       1610    1168
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AleI     AloI    AlwNI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BciVI
     BglI    BglII     BmrI     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI    CspCI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I     KasI
     MfeI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PciI    PflMI    PpuMI    PspXI     PstI
     SacI    SacII    SanDI     SapI     ScaI    SexAI     SfcI     SfoI
    SgrAI     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI
     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AlfI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbsI    BbvCI     BcgI
     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsiWI    BspHI   BssHII    BssSI     ClaI     DraI
     DrdI    EcoNI    EcoRV     FseI    FspAI   HincII  HindIII     HpaI
     KpnI     MluI     NdeI     NheI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    PvuII    RsrII     SalI     SbfI     SfiI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XmaI     ZraI
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