Restriction map for pF1KB5562
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BfrI        BglII       BlnI        BsaAI       BsaHI       
BsaI        BseAI       BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CfoI        ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
EcoNI       EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KspI        
MaeII       MfeI        MluI        MroI        MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SwaI        TfiI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AflIII       2223  ACATGT               A^C[GA][CT]GT
Alw44I        637  GTGCAC               G^TGCAC
AocI         2260  CCTGAGG              CC^T[ACGT]AGG
ApaLI         637  GTGCAC               G^TGCAC
BciVI         704  GTATCC               GTATCC(6/5)
BcnI         1781  CCCGG                CC^[GC]GG
BglI         2172  GCCACAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          843  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      843  GCTGAGC              GC^T[ACGT]AGC
BsgI          921  CTGCAC               GTGCAG(16/14)
BsiEI        1783  CGGCCG               CG[GA][CT]^CG
Bsp1407I     1051  TGTACA               T^GTACA
BspLU11I     2223  ACATGT               A^CATGT
BsrDI          60  CATTGC               GCAATG(2/0)
BsrFI        1785  GCCGGC               [GA]^CCGG[CT]
BsrGI        1051  TGTACA               T^GTACA
BstAPI        998  GCAAGTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2260  CCTGAGG              CC^T[ACGT]AGG
CelII         843  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1785  GCCGGC               [GA]^CCGG[CT]
DraII        1452  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        904  CACTTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1411  CCACGG               C^C[GA][CT]GG
EagI         1783  CGGCCG               C^GGCCG
EarI         1544  GAAGAG               CTCTTC(1/4)
EclXI        1783  CGGCCG               C^GGCCG
Eco52I       1783  CGGCCG               C^GGCCG
Eco81I       2260  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1452  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I      1890  CCAAGG               C^C[AT][AT]GG
HincII       1073  GTCAAC               GT[CT]^[GA]AC
HindII       1073  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1544  GAAGAG               CTCTTC(1/4)
NaeI         1785  GCCGGC               GCC^GGC
NciI         1781  CCCGG                CC^[GC]GG
NgoMIV       1785  GCCGGC               G^CCGGC
NspI         2223  ACATGT               [GA]CATG^[CT]
PpuMI        1452  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII        1492  CAGCTG               CAG^CTG
SacI          243  GAGCTC               GAGCT^C
SpeI         1036  ACTAGT               A^CTAGT
SspBI        1051  TGTACA               T^GTACA
SspI         1183  AATATT               AAT^ATT
StuI          938  AGGCCT               AGG^CCT
StyI         1890  CCAAGG               C^C[AT][AT]GG
TaqI          369  TCGA                 T^CGA
TthHB8I       369  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I        345  GGTACC               G^GTACC
             1536  GGTACC              
Asp718I       345  GGTACC               G^GTACC
             1536  GGTACC              
AspHI         243  GAGCTC               G[AT]GC[AT]^C
              637  GTGCAC              
BpmI         1719  CTCCAG               CTGGAG(16/14)
             1474  CTGGAG              
BsaBI         187  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1654  GATAGAGATC          
BsaWI        1280  TCCGGT               [AT]^CCGG[AT]
             1286  TCCGGT              
BseRI        1716  CTCCTC               GAGGAG(10/8)
               98  GAGGAG              
BsiHKAI       243  GAGCTC               G[AT]GC[AT]^C
              637  GTGCAC              
BsrBI        1923  CCGCTC               CCGCTC(-3/-3)
              214  GAGCGG              
HphI         1131  TCACC                GGTGA(8/7)
             2084  TCACC               
KpnI          345  GGTACC               GGTAC^C
             1536  GGTACC              
MamI          187  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1654  GATAGAGATC          
MseI          122  TTAA                 T^TAA
             1252  TTAA                
MspA1I       1134  CCGCTG               C[AC]G^C[GT]G
             1492  CAGCTG              
PflMI         979  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1941  CCAGCAGGTGG         
PstI         1844  CTGCAG               CTGCA^G
             2126  CTGCAG              
SmlI          774  CTCAAG               C^T[CT][GA]AG
             1624  CTTGAG              
Tru9I         122  TTAA                 T^TAA
             1252  TTAA                
Tsp45I        577  GTGAC                ^GT[GC]AC
             1591  GTGAC               
Van91I        979  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1941  CCAGCAGGTGG         
XcmI          389  CCATCTTACGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              598  CCACAGTCTGAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BclI        BfaI        
BmyI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EaeI        EcoRI       
EcoRII      FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp509I     TspRI       
XhoII       
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