Restriction map for pF1KB5562
 (Linear) (Six-base) MAPSORT of: KIBB5562.seq  Check: 1148  from: 1  to: 2361



KIBB5562.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     943    1935    2361 
   Size:        943     992     426

  Fragments arranged by size: 

                 992     943     426
 
Acc65I G'GTAC_C

Cuts at:      0     345    1536    2361 
   Size:        345    1191     825

  Fragments arranged by size: 

                1191     825     345
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1226    2004    2361 
   Size:        665     561     778     357

  Fragments arranged by size: 

                 778     665     561     357
 
AflIII A'CryG_T

Cuts at:      0    2223    2361 
   Size:       2223     138
 
AleI CACnn'nnGTG

Cuts at:      0    1123    1136    1387    2361 
   Size:       1123      13     251     974

  Fragments arranged by size: 

                1123     974     251      13
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     263    2361 
   Size:        263    2098
 
AlwNI CAG_nnn'CTG

Cuts at:      0     489     832     985    1843    2361 
   Size:        489     343     153     858     518

  Fragments arranged by size: 

                 858     518     489     343     153
 
ApaLI G'TGCA_C

Cuts at:      0     637    2361 
   Size:        637    1724
 
ApoI r'AATT_y

Cuts at:      0      86     172    1192    1794    2323    2361 
   Size:         86      86    1020     602     529      38

  Fragments arranged by size: 

                1020     602     529      86      86      38
 
BanI G'GyrC_C

Cuts at:      0     345    1497    1536    2041    2361 
   Size:        345    1152      39     505     320

  Fragments arranged by size: 

                1152     505     345     320      39
 
BanII G_rGCy'C

Cuts at:      0     247     775    1486    2361 
   Size:        247     528     711     875

  Fragments arranged by size: 

                 875     711     528     247
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     743    1214    2259    2361 
   Size:        743     471    1045     102

  Fragments arranged by size: 

                1045     743     471     102
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     715    2361 
   Size:        715    1646
 
BclI T'GATC_A

Cuts at:      0     192     480     630    2361 
   Size:        192     288     150    1731

  Fragments arranged by size: 

                1731     288     192     150
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2178    2361 
   Size:       2178     183
 
BlpI GC'TnA_GC

Cuts at:      0     844    2361 
   Size:        844    1517
 
Bme1580I G_kGCm'C

Cuts at:      0     363     641    2046    2361 
   Size:        363     278    1405     315

  Fragments arranged by size: 

                1405     363     315     278
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      58     820    1977    2361 
   Size:         58     762    1157     384

  Fragments arranged by size: 

                1157     762     384      58
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     261    1107    1500    2361 
   Size:        261     846     393     861

  Fragments arranged by size: 

                 861     846     393     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1495    1704    2361 
   Size:       1495     209     657

  Fragments arranged by size: 

                1495     657     209
 
Bpu10I CC'TnA_GC

Cuts at:      0    1485    1926    2361 
   Size:       1485     441     435

  Fragments arranged by size: 

                1485     441     435
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     759    1645    2361 
   Size:        759     886     716

  Fragments arranged by size: 

                 886     759     716
 
BsaBI GATnn'nnATC

Cuts at:      0     191    1658    2361 
   Size:        191    1467     703

  Fragments arranged by size: 

                1467     703     191
 
BsaWI w'CCGG_w

Cuts at:      0    1280    1286    2361 
   Size:       1280       6    1075

  Fragments arranged by size: 

                1280    1075       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      92     122    1026    1056    1755    1785    2266    2296 
   Size:         92      30     904      30     699      30     481      30

Cuts at:   2296    2361 
   Size:         65

  Fragments arranged by size: 

                 904     699     481      92      65      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     113    1707    2361 
   Size:        113    1594     654

  Fragments arranged by size: 

                1594     654     113
 
BseYI C'CCAG_C

Cuts at:      0     948    1940    2289    2361 
   Size:        948     992     349      72

  Fragments arranged by size: 

                 992     948     349      72
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     906    2361 
   Size:        906    1455
 
BsiEI CG_ry'CG

Cuts at:      0    1786    2361 
   Size:       1786     575
 
BsiHKAI G_wGCw'C

Cuts at:      0     247     641    2361 
   Size:        247     394    1720

  Fragments arranged by size: 

                1720     394     247
 
Bsp1286I G_dGCh'C

Cuts at:      0     247     363     641     775    1486    2046    2361 
   Size:        247     116     278     134     711     560     315

  Fragments arranged by size: 

                 711     560     315     278     247     134     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     943    1020    1935    2361 
   Size:        943      77     915     426

  Fragments arranged by size: 

                 943     915     426      77
 
BsrBI CCG'CTC

Cuts at:      0     216    1925    2361 
   Size:        216    1709     436

  Fragments arranged by size: 

                1709     436     216
 
BsrDI GCAATG_nn'

Cuts at:      0      59    2361 
   Size:         59    2302
 
BsrFI r'CCGG_y

Cuts at:      0    1785    2361 
   Size:       1785     576
 
BsrGI T'GTAC_A

Cuts at:      0    1051    2361 
   Size:       1051    1310
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1004    2361 
   Size:       1004    1357
 
BstYI r'GATC_y

Cuts at:      0     711    1659    2095    2361 
   Size:        711     948     436     266

  Fragments arranged by size: 

                 948     711     436     266
 
Bsu36I CC'TnA_GG

Cuts at:      0    2261    2361 
   Size:       2261     100
 
BtgI C'CryG_G

Cuts at:      0    1411    2361 
   Size:       1411     950
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2039    2361 
   Size:       2039     322
 
BtsI GCAGTG_nn'

Cuts at:      0     918    2361 
   Size:        918    1443
 
DraIII CAC_nnn'GTG

Cuts at:      0     909    2361 
   Size:        909    1452
 
EaeI y'GGCC_r

Cuts at:      0     987    1013    1556    1783    2170    2253    2361 
   Size:        987      26     543     227     387      83     108

  Fragments arranged by size: 

                 987     543     387     227     108      83      26
 
EagI C'GGCC_G

Cuts at:      0    1783    2361 
   Size:       1783     578
 
EarI CTCTTCn'nnn_

Cuts at:      0    1539    2361 
   Size:       1539     822
 
EcoICRI GAG'CTC

Cuts at:      0     245    2361 
   Size:        245    2116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665    1226    1495    1704    2004    2361 
   Size:        665     561     269     209     300     357

  Fragments arranged by size: 

                 665     561     357     300     269     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1453    2361 
   Size:       1453     908
 
EcoRI G'AATT_C

Cuts at:      0      86    1192    2323    2361 
   Size:         86    1106    1131      38

  Fragments arranged by size: 

                1131    1106      86      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     280    2361 
   Size:        280    2081
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60      92     124     184     216    2266    2298 
   Size:         28      32      32      32      60      32    2050      32

Cuts at:   2298    2361 
   Size:         63

  Fragments arranged by size: 

                2050      63      60      32      32      32      32      32
                  28
 
HincII GTy'rAC

Cuts at:      0    1075    2361 
   Size:       1075    1286
 
KpnI G_GTAC'C

Cuts at:      0     349    1540    2361 
   Size:        349    1191     821

  Fragments arranged by size: 

                1191     821     349
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1260    1451    1904    2361 
   Size:        209    1051     191     453     457

  Fragments arranged by size: 

                1051     457     453     209     191
 
MscI TGG'CCA

Cuts at:      0     989    1015    1558    2172    2255    2361 
   Size:        989      26     543     614      83     106

  Fragments arranged by size: 

                 989     614     543     106      83      26
 
MslI CAynn'nnrTG

Cuts at:      0     644     760    1123    1136    1387    2361 
   Size:        644     116     363      13     251     974

  Fragments arranged by size: 

                 974     644     363     251     116      13
 
MspA1I CmG'CkG

Cuts at:      0    1136    1494    2361 
   Size:       1136     358     867

  Fragments arranged by size: 

                1136     867     358
 
NaeI GCC'GGC

Cuts at:      0    1787    2361 
   Size:       1787     574
 
NgoMIV G'CCGG_C

Cuts at:      0    1785    2361 
   Size:       1785     576
 
NspI r_CATG'y

Cuts at:      0    2227    2361 
   Size:       2227     134
 
PasI CC'CwG_GG

Cuts at:      0    1755    2361 
   Size:       1755     606
 
PciI A'CATG_T

Cuts at:      0    2223    2361 
   Size:       2223     138
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     985    1947    2361 
   Size:        985     962     414

  Fragments arranged by size: 

                 985     962     414
 
PfoI T'CCnGG_A

Cuts at:      0     707    2270    2361 
   Size:        707    1563      91

  Fragments arranged by size: 

                1563     707      91
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1333    1365    2361 
   Size:       1333      32     996

  Fragments arranged by size: 

                1333     996      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1453    2361 
   Size:       1453     908
 
PstI C_TGCA'G

Cuts at:      0    1848    2130    2361 
   Size:       1848     282     231

  Fragments arranged by size: 

                1848     282     231
 
PvuII CAG'CTG

Cuts at:      0    1494    2361 
   Size:       1494     867
 
SacI G_AGCT'C

Cuts at:      0     247    2361 
   Size:        247    2114
 
SfcI C'TryA_G

Cuts at:      0     354    1844    2126    2361 
   Size:        354    1490     282     235

  Fragments arranged by size: 

                1490     354     282     235
 
SmlI C'TyrA_G

Cuts at:      0     774    1624    2361 
   Size:        774     850     737

  Fragments arranged by size: 

                 850     774     737
 
SpeI A'CTAG_T

Cuts at:      0    1036    2361 
   Size:       1036    1325
 
SspI AAT'ATT

Cuts at:      0    1185    2361 
   Size:       1185    1176
 
StuI AGG'CCT

Cuts at:      0     940    2361 
   Size:        940    1421
 
StyI C'CwwG_G

Cuts at:      0    1890    2361 
   Size:       1890     471
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     656    1515    1753    1903    2152    2361 
   Size:        656     859     238     150     249     209

  Fragments arranged by size: 

                 859     656     249     238     209     150
 
TatI w'GTAC_w

Cuts at:      0    1051    1364    2361 
   Size:       1051     313     997

  Fragments arranged by size: 

                1051     997     313
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     403     435    1755    1787    2361 
   Size:        403      32    1320      32     574

  Fragments arranged by size: 

                1320     574     403      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     396     605    2361 
   Size:        396     209    1756

  Fragments arranged by size: 

                1756     396     209
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     BanI    BanII     BbsI    BciVI     BclI     BglI     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PpiI    PpuMI     PstI    PvuII     SacI     SfcI     SmlI
     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BfrBI    BglII    BmgBI     BmtI     BsaI
    BsaAI    BsaHI    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI
     DrdI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SphI     SrfI     SwaI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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