Restriction map for pF1KB5485
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AviII       
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BfaI        BfrI        BlnI        BseAI       BsiEI       
BsiHKAI     BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HinfI       
HpaI        KpnI        KspI        MaeI        MfeI        
MluI        MroI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI          555  GAATTT               [GA]^AATT[CT]
AcyI          278  GGCGCC               G[GA]^CG[CT]C
AlwNI        1267  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          555  GAATTT               [GA]^AATT[CT]
Asp700I       153  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          418  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          662  TGGCCA               TGG^CCA
BanI          278  GGCGCC               G^G[CT][GA]CC
BbeI          278  GGCGCC               GGCGC^C
BcgI         1226  GCATGACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          671  TGATCA               T^GATCA
BglII        1247  AGATCT               A^GATCT
BlpI          631  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      631  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1320  CACGTA               [CT]AC^GT[GA]
BsaBI        1263  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         278  GGCGCC               G[GA]^CG[CT]C
BsaI          367  GAGACC               GGTCTC(1/5)
BsiWI        1322  CGTACG               C^GTACG
BsmI          775  GCATTC               GAATGC(1/-1)
BspMI        1080  GCAGGT               ACCTGC(4/8)
BssHII          6  GCGCGC               G^CGCGC
CelII         631  GCTGAGC              GC^T[ACGT]AGC
DraIII       1398  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          864  GAAGAG               CTCTTC(1/4)
FbaI          671  TGATCA               T^GATCA
HaeII         278  GGCGCC               [GA]GCGC^[CT]
HgaI          289  GACGC                GACGC(5/10)
Hin1I         278  GGCGCC               G[GA]^CG[CT]C
KasI          278  GGCGCC               G^GCGCC
Ksp632I       864  GAAGAG               CTCTTC(1/4)
MamI         1263  GATGCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         662  TGGCCA               TGG^CCA
MscI          662  TGGCCA               TGG^CCA
MseI         1100  TTAA                 T^TAA
NarI          278  GGCGCC               GG^CGCC
PflFI         418  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1495  CTGCAG               CTGCA^G
SapI          864  GAAGAGC              GCTCTTC(1/4)
SexAI         404  ACCTGGT              A^CC[AT]GGT
SfcI         1495  CTGCAG               C^T[GA][CT]AG
SfiI          243  GGCCGCCCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          278  GGCGCC               GGC^GCC
SmlI          835  CTCAAG               C^T[CT][GA]AG
TaqI          335  TCGA                 T^CGA
Tru9I        1100  TTAA                 T^TAA
Tth111I       418  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       335  TCGA                 T^CGA
XmnI          153  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII           7  CGCG                 CG^CG
              802  CGCG                
AlwI         1107  GATCC                GGATC(4/5)
             1269  GATCC               
ApaI           25  GGGCCC               GGGCC^C
              588  GGGCCC              
BbsI          100  GTCTTC               GAAGAC(2/6)
             1342  GTCTTC              
BpmI         1387  CTCCAG               CTGGAG(16/14)
              182  CTGGAG              
BpuAI         100  GTCTTC               GAAGAC(2/6)
             1342  GTCTTC              
BsaWI         344  ACCGGA               [AT]^CCGG[AT]
              437  TCCGGT              
BseRI        1243  GAGGAG               GAGGAG(10/8)
             1291  GAGGAG              
BsgI          979  GTGCAG               GTGCAG(16/14)
             1216  GTGCAG              
BsrDI         744  CATTGC               GCAATG(2/0)
             1167  CATTGC              
BstUI           7  CGCG                 CG^CG
              802  CGCG                
DraII          25  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1382  AGGCCCT             
EcoNI         669  CCTGATCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1374  CCTGTCCCAGG         
EcoO109I       25  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1382  AGGCCCT             
HincII        703  GTCAAC               GT[CT]^[GA]AC
             1408  GTCAAC              
HindII        703  GTCAAC               GT[CT]^[GA]AC
             1408  GTCAAC              
MaeII         611  ACGT                 A^CGT
             1321  ACGT                
MspA1I        262  CAGCTG               C[AC]G^C[GT]G
             1337  CAGCTG              
MvnI            7  CGCG                 CG^CG
              802  CGCG                
PvuII         262  CAGCTG               CAG^CTG
             1337  CAGCTG              
SmaI          248  CCCGGG               CCC^GGG
             1022  CCCGGG              
XmaCI         248  CCCGGG               C^CCGGG
             1022  CCCGGG              
XmaI          248  CCCGGG               C^CCGGG
             1022  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspI        MvaI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TseI        Tsp45I      Tsp509I     TspRI       XcmI        
XhoII       
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