Restriction map for pF1KB5485
 (Circular) (Six-base) MAPSORT of: pF1KB5485.seq  Check: 5636  from: 1  to: 4664



pF1KB5485  4664 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1179    1179 
   Size:       4664
 
AccI GT'mk_AC

Cuts at:   1702    1702 
   Size:       4664
 
Acc65I G'GTAC_C

Cuts at:   1680    1680 
   Size:       4664
 
AclI AA'CG_TT

Cuts at:   4504    4504 
   Size:       4664
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     594    1402    1637    2489    2921    3274    3936     335 
   Size:        259     808     235     852     432     353     662    1063

  Fragments arranged by size: 

                1063     852     808     662     432     353     259     235
            
 
AflIII A'CryG_T

Cuts at:   2016    3816    4097    2016 
   Size:       1800     281    2583

  Fragments arranged by size: 

                2583    1800     281
 
AleI CACnn'nnGTG

Cuts at:   1618    1618 
   Size:       4664
 
AlwNI CAG_nnn'CTG

Cuts at:   1380    1969    3407    1380 
   Size:        589    1438    2637

  Fragments arranged by size: 

                2637    1438     589
 
ApaI G_GGCC'C

Cuts at:    137     700     137 
   Size:        563    4101
 
ApaLI G'TGCA_C

Cuts at:   3502    3502 
   Size:       4664
 
ApoI r'AATT_y

Cuts at:    663    1668    4017    4273     663 
   Size:       1005    2349     256    1054

  Fragments arranged by size: 

                2349    1054    1005     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4664
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4664
 
AvaI C'yCGr_G

Cuts at:    202     356    1130    1142    1684     202 
   Size:        154     774      12     542    3182

  Fragments arranged by size: 

                3182     774     542     154      12
 
BamHI G'GATC_C

Cuts at:   1689    1689 
   Size:       4664
 
BanI G'GyrC_C

Cuts at:    386    1680    2094    2342    2377     386 
   Size:       1294     414     248      35    2673

  Fragments arranged by size: 

                2673    1294     414     248      35
 
BanII G_rGCy'C

Cuts at:    137     379     700     979    1542    1678    2708     137 
   Size:        242     321     279     563     136    1030    2093

  Fragments arranged by size: 

                2093    1030     563     321     279     242     136
 
BbeI G_GCGC'C

Cuts at:    390    2098    2346     390 
   Size:       1708     248    2708

  Fragments arranged by size: 

                2708    1708     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    201    1443     201 
   Size:       1242    3422
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1323    1357    1323 
   Size:         34    4630
 
BciVI GTATCCnnnnn_n'

Cuts at:   2562    3618    4664    2562 
   Size:       1056    1046    2562

  Fragments arranged by size: 

                2562    1056    1046
 
BclI T'GATC_A

Cuts at:    779    2184     779 
   Size:       1405    3259
 
BglI GCCn_nnn'nGGC

Cuts at:    175     358     562    1498     175 
   Size:        183     204     936    3341

  Fragments arranged by size: 

                3341     936     204     183
 
BglII A'GATC_T

Cuts at:   1355    2179    4216    1355 
   Size:        824    2037    1803

  Fragments arranged by size: 

                2037    1803     824
 
BlpI GC'TnA_GC

Cuts at:    740    1776     740 
   Size:       1036    3628
 
Bme1580I G_kGCm'C

Cuts at:    137     700    1507    2289    2382    3506     137 
   Size:        563     807     782      93    1124    1295

  Fragments arranged by size: 

                1295    1124     807     782     563      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1536    1876    1957    2292    4436    1536 
   Size:        340      81     335    2144    1764

  Fragments arranged by size: 

                2144    1764     340     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3031    3031 
   Size:       4664
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    311    1480     311 
   Size:       1169    3495
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    928    1840    2719    3433    3731     928 
   Size:        912     879     714     298    1861

  Fragments arranged by size: 

                1861     912     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    469    4368     469 
   Size:       3899     765
 
BsaAI yAC'GTr

Cuts at:   1430    2017    1430 
   Size:        587    4077
 
BsaBI GATnn'nnATC

Cuts at:      6    1375    2178    4215       6 
   Size:       1369     803    2037     455

  Fragments arranged by size: 

                2037    1369     803     455
 
BsaHI Gr'CG_yC

Cuts at:    387    2095    2343    3045    4541     387 
   Size:       1708     248     702    1496     510

  Fragments arranged by size: 

                1708    1496     702     510     248
 
BsaWI w'CCGG_w

Cuts at:    452     545    1853    2374    3052    3463    3610    3884     452 
   Size:         93    1308     521     678     411     147     274    1232

  Fragments arranged by size: 

                1308    1232     678     521     411     274     147      93
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1366    1414    1366 
   Size:         48    4616
 
BseYI C'CCAG_C

Cuts at:    372     560     693     699     841     928     963    2089    2847 
   Size:        188     133       6     142      87      35    1126     758

Cuts at:   2847    3512    3920     372 
   Size:        665     408    1116

  Fragments arranged by size: 

                1126    1116     758     665     408     188     142     133
                  87      35       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1108    1345    1108 
   Size:        237    4427
 
BsiEI CG_ry'CG

Cuts at:    104    2206    2252    3482     104 
   Size:       2102      46    1230    1286

  Fragments arranged by size: 

                2102    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1678    2456    2646    3506    1678 
   Size:        778     190     860    2836

  Fragments arranged by size: 

                2836     860     778     190
 
BsiWI C'GTAC_G

Cuts at:   1430    1430 
   Size:       4664
 
BsmI GAATG_Cn'

Cuts at:    883     883 
   Size:       4664
 
Bsp1286I G_dGCh'C

Cuts at:    137     379     700     979    1507    1542    1678    2289    2382 
   Size:        242     321     279     528      35     136     611      93

Cuts at:   2382    2456    2646    2708    3506     137 
   Size:         74     190      62     798    1295

  Fragments arranged by size: 

                1295     798     611     528     321     279     242     190
                 136      93      74      62      35
 
BspHI T'CATG_A

Cuts at:   3096    3096 
   Size:       4664
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1179    1714    1928    2230    2611    1179 
   Size:        535     214     302     381    3232

  Fragments arranged by size: 

                3232     535     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     447     672    1119    2956    3010    4494    4659      50 
   Size:        397     225     447    1837      54    1484     165      55

  Fragments arranged by size: 

                1837    1484     447     397     225     165      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:    851    1274    2576     851 
   Size:        423    1302    2939

  Fragments arranged by size: 

                2939    1302     423
 
BsrFI r'CCGG_y

Cuts at:    413     556    1069    2662    2843     413 
   Size:        143     513    1593     181    2234

  Fragments arranged by size: 

                2234    1593     513     181     143
 
BssHII G'CGCG_C

Cuts at:    114     114 
   Size:       4664
 
BssSI C'ACGA_G

Cuts at:   2935    3643    2935 
   Size:        708    3956
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2041    2041 
   Size:       4664
 
BstBI TT'CG_AA

Cuts at:   3025    3025 
   Size:       4664
 
BstYI r'GATC_y

Cuts at:      1    1214    1355    1376    1689    2156    2179    2514    2760 
   Size:       1213     141      21     313     467      23     335     246

Cuts at:   2760    3066    3078    3164    4216       1 
   Size:        306      12      86    1052     449

  Fragments arranged by size: 

                1213    1052     467     449     335     313     306     246
                 141      86      23      21      12
 
BtgI C'CryG_G

Cuts at:    576     658     836    1016    1287     576 
   Size:         82     178     180     271    3953

  Fragments arranged by size: 

                3953     271     180     178      82
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1326    2614    2795    2958    1326 
   Size:       1288     181     163    3032

  Fragments arranged by size: 

                3032    1288     181     163
 
BtsI GCAGTG_nn'

Cuts at:    350    1907     350 
   Size:       1557    3107
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    885     920    1296    1331     885 
   Size:         35     376      35    4218

  Fragments arranged by size: 

                4218     376      35      35
 
DraI TTT'AAA

Cuts at:   1663    1663 
   Size:       4664
 
DraIII CAC_nnn'GTG

Cuts at:   1511    1511 
   Size:       4664
 
DrdI GACnn_nn'nnGTC

Cuts at:   2370    3714    2370 
   Size:       1344    3320
 
EaeI y'GGCC_r

Cuts at:    431     770     906    1439    1578    2249    2423    2814    2841 
   Size:        339     136     533     139     671     174     391      27

Cuts at:   2841    4186     431 
   Size:       1345     909

  Fragments arranged by size: 

                1345     909     671     533     391     339     174     139
                 136      27
 
EagI C'GGCC_G

Cuts at:   2249    2249 
   Size:       4664
 
EarI CTCTTCn'nnn_

Cuts at:    967    2687    2897     967 
   Size:       1720     210    2734

  Fragments arranged by size: 

                2734    1720     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2872    3614    3760    4474    2872 
   Size:        742     146     714    3062

  Fragments arranged by size: 

                3062     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1676    1676 
   Size:       4664
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    311     335     594    1402    1480    1637    2489    2921    3274 
   Size:         24     259     808      78     157     852     432     353

Cuts at:   3274    3936     311 
   Size:        662    1039

  Fragments arranged by size: 

                1039     852     808     662     432     353     259     157
                  78      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:    781    1486     781 
   Size:        705    3959
 
EcoO109I rG'GnC_Cy

Cuts at:    134    1491    1803     134 
   Size:       1357     312    2995

  Fragments arranged by size: 

                2995    1357     312
 
EcoRI G'AATT_C

Cuts at:   1668    1668 
   Size:       4664
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2683    2683 
   Size:       4664
 
FspI TGC'GCA

Cuts at:   2445    2445 
   Size:       4664
 
HaeII r_GCGC'y

Cuts at:    390    2098    2346    3576    4347     390 
   Size:       1708     248    1230     771     707

  Fragments arranged by size: 

                1708    1230     771     707     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    284     316    2506    2536    2538    2568     284 
   Size:         32    2190      30       2      30    2380

  Fragments arranged by size: 

                2380    2190      32      30      30       2
 
HincII GTy'rAC

Cuts at:    813    1518    1703     813 
   Size:        705     185    3774

  Fragments arranged by size: 

                3774     705     185
 
KasI G'GCGC_C

Cuts at:    386    2094    2342     386 
   Size:       1708     248    2708

  Fragments arranged by size: 

                2708    1708     248
 
KpnI G_GTAC'C

Cuts at:   1684    1684 
   Size:       4664
 
MluI A'CGCG_T

Cuts at:   4097    4097 
   Size:       4664
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3423    3607    3423 
   Size:        184    4480
 
MscI TGG'CCA

Cuts at:    772    2425     772 
   Size:       1653    3011
 
MslI CAynn'nnrTG

Cuts at:    866     988    1618    2653    2780     866 
   Size:        122     630    1035     127    2750

  Fragments arranged by size: 

                2750    1035     630     127     122
 
MspA1I CmG'CkG

Cuts at:    372    1447    1775    2089    2449    3233    3478     372 
   Size:       1075     328     314     360     784     245    1558

  Fragments arranged by size: 

                1558    1075     784     360     328     314     245
 
NaeI GCC'GGC

Cuts at:    415     558    1071    2845     415 
   Size:        143     513    1774    2234

  Fragments arranged by size: 

                2234    1774     513     143
 
NarI GG'CG_CC

Cuts at:    387    2095    2343     387 
   Size:       1708     248    2708

  Fragments arranged by size: 

                2708    1708     248
 
NgoMIV G'CCGG_C

Cuts at:    413     556    1069    2843     413 
   Size:        143     513    1774    2234

  Fragments arranged by size: 

                2234    1774     513     143
 
NspI r_CATG'y

Cuts at:   1717    2217    3820    1717 
   Size:        500    1603    2561

  Fragments arranged by size: 

                2561    1603     500
 
PciI A'CATG_T

Cuts at:   3816    3816 
   Size:       4664
 
PfoI T'CCnGG_A

Cuts at:   1075    1075 
   Size:       4664
 
PmeI GTTT'AAAC

Cuts at:   1663    1663 
   Size:       4664
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3110    3142    3110 
   Size:         32    4632
 
PspOMI G'GGCC_C

Cuts at:    133     696     133 
   Size:        563    4101
 
PstI C_TGCA'G

Cuts at:   1607    1711    2396    1607 
   Size:        104     685    3875

  Fragments arranged by size: 

                3875     685     104
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4664
 
PvuII CAG'CTG

Cuts at:    372    1447    2089    2449     372 
   Size:       1075     642     360    2587

  Fragments arranged by size: 

                2587    1075     642     360
 
RsrII CG'GwC_CG

Cuts at:   2859    2859 
   Size:       4664
 
SacI G_AGCT'C

Cuts at:   1678    1678 
   Size:       4664
 
SalI G'TCGA_C

Cuts at:   1701    1701 
   Size:       4664
 
SapI GCTCTTCn'nnn_

Cuts at:    967    2687    2897     967 
   Size:       1720     210    2734

  Fragments arranged by size: 

                2734    1720     210
 
SbfI CC_TGCA'GG

Cuts at:   1711    1711 
   Size:       4664
 
ScaI AGT'ACT

Cuts at:   4181    4181 
   Size:       4664
 
SexAI A'CCwGG_T

Cuts at:    512     512 
   Size:       4664
 
SfcI C'TryA_G

Cuts at:     33    1603    1707    2392    3360    3551      33 
   Size:       1570     104     685     968     191    1146

  Fragments arranged by size: 

                1570    1146     968     685     191     104
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    358     358 
   Size:       4664
 
SfoI GGC'GCC

Cuts at:    388    2096    2344     388 
   Size:       1708     248    2708

  Fragments arranged by size: 

                2708    1708     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4664
 
SmaI CCC'GGG

Cuts at:    358    1132    1686     358 
   Size:        774     554    3336

  Fragments arranged by size: 

                3336     774     554
 
SmlI C'TyrA_G

Cuts at:    943    1819    2734    3448    3710     943 
   Size:        876     915     714     262    1897

  Fragments arranged by size: 

                1897     915     876     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4664
 
SphI G_CATG'C

Cuts at:   1717    2217    1717 
   Size:        500    4164
 
SrfI GCCC'GGGC

Cuts at:    358    1132     358 
   Size:        774    3890
 
StyI C'CwwG_G

Cuts at:    382     470     704     956    1346    1798    2152     382 
   Size:         88     234     252     390     452     354    2894

  Fragments arranged by size: 

                2894     452     390     354     252     234      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    187    1189    2247    2380    2839    3048     187 
   Size:       1002    1058     133     459     209    1803

  Fragments arranged by size: 

                1803    1058    1002     459     209     133
 
TatI w'GTAC_w

Cuts at:   4179    4179 
   Size:       4664
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    264     296    4181    4213     264 
   Size:         32    3885      32     715

  Fragments arranged by size: 

                3885     715      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    529    2461     529 
   Size:       1932    2732
 
XbaI T'CTAG_A

Cuts at:   1695    1695 
   Size:       4664
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    925    1143    1434    1549    2159     925 
   Size:        218     291     115     610    3430

  Fragments arranged by size: 

                3430     610     291     218     115
 
XmaI C'CCGG_G

Cuts at:    356    1130    1684     356 
   Size:        774     554    3336

  Fragments arranged by size: 

                3336     774     554
 
XmnI GAAnn'nnTTC

Cuts at:    265     265 
   Size:       4664
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AleI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI
     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SrfI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AgeI     AhdI     AlfI     AloI     AscI
    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I    BsaXI
    BsmBI    BspEI    BsrGI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SgrAI
    SnaBI     SspI     StuI     SwaI     XhoI     ZraI
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