Restriction map for pF1KB5398
##################################
# NO CUT ENZYMES (  141 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspI        AvaI        
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfaI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DrdI        Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        HgaI        
HincII      HindII      HinfI       HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MunI        MvnI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
TaqI        TfiI        Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AccIII        530  TCCGGA               T^CCGGA
AcyI          233  GGCGCC               G[GA]^CG[CT]C
AflII         109  CTTAAG               C^TTAAG
AhdI          511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         450  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I         6  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         384  GGACC                G^G[AT]CC
AviII          53  TGCGCA               TGC^GCA
BalI          207  TGGCCA               TGG^CCA
BanI          233  GGCGCC               G^G[CT][GA]CC
BbeI          233  GGCGCC               GGCGC^C
BbsI           87  GAAGAC               GAAGAC(2/6)
BcnI          348  CCGGG                CC^[GC]GG
BfrI          109  CTTAAG               C^TTAAG
BglI          225  GCCCCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI          87  GAAGAC               GAAGAC(2/6)
BsaHI         233  GGCGCC               G[GA]^CG[CT]C
BsaWI         530  TCCGGA               [AT]^CCGG[AT]
BseAI         530  TCCGGA               T^CCGGA
BseRI         313  GAGGAG               GAGGAG(10/8)
BsgI          433  CTGCAC               GTGCAG(16/14)
BsiEI         407  CGGCCG               CG[GA][CT]^CG
BsmFI         197  GGGAC                GGGAC(10/14)
BsmI          402  GAATGC               GAATGC(1/-1)
BspEI         530  TCCGGA               T^CCGGA
BspHI         320  TCATGA               T^CATGA
BspMI         472  ACCTGC               ACCTGC(4/8)
BsrDI         281  GCAATG               GCAATG(2/0)
BstYI         218  AGATCC               [GA]^GATC[CT]
Cac8I         330  GCTGGC               GC[ACGT]^[ACGT]GC
DraIII        189  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          164  CCGTGG               C^C[GA][CT]GG
EagI          407  CGGCCG               C^GGCCG
Eam1105I      511  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           22  CTCTTC               CTCTTC(1/4)
EclXI         407  CGGCCG               C^GGCCG
Eco52I        407  CGGCCG               C^GGCCG
EcoNI          21  CCTCTTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI           53  TGCGCA               TGC^GCA
HaeII         233  GGCGCC               [GA]GCGC^[CT]
Hin1I         233  GGCGCC               G[GA]^CG[CT]C
HindIII       106  AAGCTT               A^AGCTT
HphI          118  GGTGA                GGTGA(8/7)
KasI          233  GGCGCC               G^GCGCC
Ksp632I        22  CTCTTC               CTCTTC(1/4)
MaeIII        127  GTGAC                ^GT[ACGT]AC
MflI          218  AGATCC               [GA]^GATC[CT]
MluNI         207  TGGCCA               TGG^CCA
MroI          530  TCCGGA               T^CCGGA
MscI          207  TGGCCA               TGG^CCA
MseI          110  TTAA                 T^TAA
MspA1I        483  CAGCGG               C[AC]G^C[GT]G
NarI          233  GGCGCC               GG^CGCC
NciI          348  CCGGG                CC^[GC]GG
PpuMI         383  AGGACCT              [GA]G^G[AT]CC[CT]
PstI           80  CTGCAG               CTGCA^G
RcaI          320  TCATGA               T^CATGA
SfcI           80  CTGCAG               C^T[GA][CT]AG
SfoI          233  GGCGCC               GGC^GCC
SmlI          109  CTTAAG               C^T[CT][GA]AG
StuI          258  AGGCCT               AGG^CCT
Tru9I         110  TTAA                 T^TAA
Tsp45I        127  GTGAC                ^GT[GC]AC
TspRI         189  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         218  AGATCC               [GA]^GATC[CT]
XmnI            6  GAACATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI          406  GCGG                 CCGC(-3/-1)
              485  GCGG                
AfaI          171  GTAC                 GT^AC
              459  GTAC                
AlwI           96  GATCC                GGATC(4/5)
              219  GATCC               
AspHI         316  GAGCTC               G[AT]GC[AT]^C
              343  GAGCTC              
BbvI           79  GCTGC                GCAGC(8/12)
              432  GCTGC               
BsaJI         164  CCGTGG               C^C[ACGT][ACGT]GG
              335  CCGAGG              
BsiHKAI       316  GAGCTC               G[AT]GC[AT]^C
              343  GAGCTC              
DraII          29  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              383  AGGACCT             
EcoO109I       29  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              383  AGGACCT             
MaeII         155  ACGT                 A^CGT
              200  ACGT                
MslI           57  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              321  CATGAGGATG          
RsaI          171  GTAC                 GT^AC
              459  GTAC                
SacI          316  GAGCTC               GAGCT^C
              343  GAGCTC              
TseI           79  GCTGC                G^C[AT]GC
              432  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AluI        BanII       BmyI        BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
CfoI        Cfr13I      DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfaNI       
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