Restriction map for pF1KB5383
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        Eam1105I    Eco81I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HpaI        KpnI        MaeII       
MamI        MluI        MluNI       MroI        MscI        
MslI        NcoI        NdeI        NheI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AcyI           64  GGCGCC               G[GA]^CG[CT]C
AfaI          359  GTAC                 GT^AC
AflIII        449  ACATGT               A^C[GA][CT]GT
AlwI           96  GATCC                GGATC(4/5)
AlwNI        1528  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1205  AGCGCT               AGC^GCT
AseI         1477  ATTAAT               AT^TAAT
AsnI         1477  ATTAAT               AT^TAAT
Asp700I      1570  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI            4  CCCGAG               C^[CT]CG[GA]G
BanII        1256  GGGCTC               G[GA]GC[CT]^C
BbeI           64  GGCGCC               GGCGC^C
BbsI          991  GAAGAC               GAAGAC(2/6)
BbvCI        1084  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1393  CTAG                 C^TAG
BglII         609  AGATCT               A^GATCT
BlpI         1201  GCTGAGC              GC^T[ACGT]AGC
BpmI         1280  CTCCAG               CTGGAG(16/14)
Bpu1102I     1201  GCTGAGC              GC^T[ACGT]AGC
BpuAI         991  GAAGAC               GAAGAC(2/6)
BsaHI          64  GGCGCC               G[GA]^CG[CT]C
BseRI        1087  GAGGAG               GAGGAG(10/8)
BsiEI          51  CGGCCG               CG[GA][CT]^CG
BsmAI         453  GTCTC                GTCTC(1/5)
BsmFI         646  GGGAC                GGGAC(10/14)
BsoBI           4  CCCGAG               C^[CT]CG[GA]G
BspHI        1215  TCATGA               T^CATGA
BspLU11I      449  ACATGT               A^CATGT
BsrBI          24  GAGCGG               CCGCTC(-3/-3)
BsrDI         333  CATTGC               GCAATG(2/0)
CelII        1201  GCTGAGC              GC^T[ACGT]AGC
DraII         416  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII       1325  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          444  GACTGACATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           54  CCGCGG               C^C[GA][CT]GG
EaeI           51  CGGCCG               [CT]^GGCC[GA]
EagI           51  CGGCCG               C^GGCCG
EclXI          51  CGGCCG               C^GGCCG
Eco47III     1205  AGCGCT               AGC^GCT
Eco52I         51  CGGCCG               C^GGCCG
EcoNI         420  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      416  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1000  GAATTC               G^AATTC
EcoT14I       153  CCTTGG               C^C[AT][AT]GG
Hin1I          64  GGCGCC               G[GA]^CG[CT]C
KasI           64  GGCGCC               G^GCGCC
KspI           54  CCGCGG               CCGC^GG
MaeI         1393  CTAG                 C^TAG
MspA1I         54  CCGCGG               C[AC]G^C[GT]G
NarI           64  GGCGCC               GG^CGCC
NotI           50  GCGGCCGC             GC^GGCCGC
PshBI        1477  ATTAAT               AT^TAAT
PstI         1469  CTGCAG               CTGCA^G
RcaI         1215  TCATGA               T^CATGA
RsaI          359  GTAC                 GT^AC
SacII          54  CCGCGG               CCGC^GG
SfoI           64  GGCGCC               GGC^GCC
SphI         1449  GCATGC               GCATG^C
SspI         1313  AATATT               AAT^ATT
StyI          153  CCTTGG               C^C[AT][AT]GG
SwaI         1097  ATTTAAAT             ATTT^AAAT
Tsp45I        473  GTGAC                ^GT[GC]AC
XmnI         1570  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          55  CGCG                 CG^CG
               79  CGCG                
AspHI        1277  GTGCTC               G[AT]GC[AT]^C
             1550  GTGCTC              
BcnI           20  CCCGG                CC^[GC]GG
               38  CCGGG               
BsiHKAI      1277  GTGCTC               G[AT]GC[AT]^C
             1550  GTGCTC              
BsmI          266  GAATGC               GAATGC(1/-1)
             1583  GCATTC              
BstUI          55  CGCG                 CG^CG
               79  CGCG                
BstXI         263  CCAGAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1502  CCAACATTTTGG        
BstYI          95  AGATCC               [GA]^GATC[CT]
              609  AGATCT              
CfoI           65  GCGC                 GCG^C
             1206  GCGC                
DraI          849  TTTAAA               TTT^AAA
             1098  TTTAAA              
EarI          832  GAAGAG               CTCTTC(1/4)
             1251  GAAGAG              
FauI           26  GCGGG                CCCGC(4/6)
               56  GCGGG               
HaeII          64  GGCGCC               [GA]GCGC^[CT]
             1205  AGCGCT              
HhaI           65  GCGC                 GCG^C
             1206  GCGC                
HinP1I         65  GCGC                 G^CGC
             1206  GCGC                
HindIII       364  AAGCTT               A^AGCTT
              637  AAGCTT              
Ksp632I       832  GAAGAG               CTCTTC(1/4)
             1251  GAAGAG              
MflI           95  AGATCC               [GA]^GATC[CT]
              609  AGATCT              
MvnI           55  CGCG                 CG^CG
               79  CGCG                
NaeI           46  GCCGGC               GCC^GGC
             1405  GCCGGC              
NciI           20  CCCGG                CC^[GC]GG
               38  CCGGG               
NgoMIV         46  GCCGGC               G^CCGGC
             1405  GCCGGC              
PleI         1557  GACTC                GAGTC(4/5)
              403  GAGTC               
SfcI         1424  CTACAG               C^T[GA][CT]AG
             1469  CTGCAG              
SmlI          290  CTCAAG               C^T[CT][GA]AG
             1120  CTCAAG              
TaqI          456  TCGA                 T^CGA
             1614  TCGA                
TthHB8I       456  TCGA                 T^CGA
             1614  TCGA                
XcmI          263  CCAGAATGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              767  CCAAAACAGACATGG     
XhoII          95  AGATCC               [GA]^GATC[CT]
              609  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        AvaII       
BanI        BbvI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr10I      Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MfeI        
MnlI        MseI        MspI        MunI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
Sau3AI      Sau96I      ScrFI       SfaNI       TfiI        
Tru9I       TseI        Tsp509I     TspRI       
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