Restriction map for pF1KB5335
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BcgI        BfaI        BfrI        
BglI        BglII       BlnI        BsaBI       BsaHI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CfoI        ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Van91I      XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AfaI           75  GTAC                 GT^AC
AlwNI        1065  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          363  GGGCCC               GGGCC^C
Asp700I       741  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         229  GAGCTC               G[AT]GC[AT]^C
AspI         1875  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1746  TGGCCA               TGG^CCA
BamHI         357  GGATCC               G^GATCC
BanI          445  GGTGCC               G^G[CT][GA]CC
BbvCI        1857  CCTCAGC              CCTCAGC(-5/-2)
BciVI         698  GGATAC               GTATCC(6/5)
BclI          417  TGATCA               T^GATCA
BlpI           51  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       51  GCTGAGC              GC^T[ACGT]AGC
BseRI        1045  GAGGAG               GAGGAG(10/8)
BsiHKAI       229  GAGCTC               G[AT]GC[AT]^C
BsmFI         371  GGGAC                GGGAC(10/14)
BsmI            4  GCATTC               GAATGC(1/-1)
Bsp1407I       74  TGTACA               T^GTACA
BspMI         680  ACCTGC               ACCTGC(4/8)
BsrGI          74  TGTACA               T^GTACA
BstAPI        285  GCAGCATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII          51  GCTGAGC              GC^T[ACGT]AGC
DraII         429  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1475  CCATGG               C^C[GA][CT]GG
EcoO109I      429  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI        1260  GAATTC               G^AATTC
FbaI          417  TGATCA               T^GATCA
MfeI          803  CAATTG               C^AATTG
MluNI        1746  TGGCCA               TGG^CCA
MscI         1746  TGGCCA               TGG^CCA
MseI          833  TTAA                 T^TAA
MunI          803  CAATTG               C^AATTG
NcoI         1475  CCATGG               C^CATGG
NdeI         1811  CATATG               CA^TATG
PaeR7I       1629  CTCGAG               C^TCGAG
PflFI        1875  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         429  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI           75  GTAC                 GT^AC
SacI          229  GAGCTC               GAGCT^C
SapI          759  GAAGAGC              GCTCTTC(1/4)
SexAI         107  ACCTGGT              A^CC[AT]GGT
SfcI           99  CTACAG               C^T[GA][CT]AG
SspBI          74  TGTACA               T^GTACA
TfiI         1942  GATTC                G^A[AT]TC
Tru9I         833  TTAA                 T^TAA
Tth111I      1875  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1629  CTCGAG               C^TCGAG
XmnI          741  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI          433  CCTGAGG              CC^T[ACGT]AGG
              583  CCTGAGG             
AvaI          789  CTCGGG               C^[CT]CG[GA]G
             1629  CTCGAG              
BbsI         1050  GAAGAC               GAAGAC(2/6)
             1872  GAAGAC              
BpuAI        1050  GAAGAC               GAAGAC(2/6)
             1872  GAAGAC              
BsaAI         721  TACGTG               [CT]AC^GT[GA]
              973  TACGTG              
BsaWI         836  ACCGGA               [AT]^CCGG[AT]
              874  TCCGGT              
BsoBI         789  CTCGGG               C^[CT]CG[GA]G
             1629  CTCGAG              
BspLU11I      890  ACATGT               A^CATGT
             1646  ACATGT              
BsrFI         201  ACCGGC               [GA]^CCGG[CT]
             1600  GCCGGT              
Bsu36I        433  CCTGAGG              CC^T[ACGT]AGG
              583  CCTGAGG             
Cfr10I        201  ACCGGC               [GA]^CCGG[CT]
             1600  GCCGGT              
EaeI         1410  TGGCCG               [CT]^GGCC[GA]
             1746  TGGCCA              
Eco81I        433  CCTGAGG              CC^T[ACGT]AGG
              583  CCTGAGG             
EcoT14I      1475  CCATGG               C^C[AT][AT]GG
             1749  CCAAGG              
PleI           69  GACTC                GAGTC(4/5)
              480  GACTC               
PvuII         154  CAGCTG               CAG^CTG
             1013  CAGCTG              
SfaNI          87  GATGC                GCATC(5/9)
             1163  GATGC               
StyI         1475  CCATGG               C^C[AT][AT]GG
             1749  CCAAGG              
TaqI          239  TCGA                 T^CGA
             1630  TCGA                
TthHB8I       239  TCGA                 T^CGA
             1630  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AcsI        AflIII      AluI        AlwI        
ApoI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaI        BsaJI       BsgI        
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        Sau3AI      
Sau96I      ScrFI       SmlI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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