Restriction map for pF1KB5335
 (Linear) (Six-base) MAPSORT of: KIBB5335.seq  Check: 5319  from: 1  to: 1959



KIBB5335.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     648     732     900     984    1068    1152    1236    1404 
   Size:        648      84     168      84      84      84      84     168

Cuts at:   1404    1488    1824    1959 
   Size:         84     336     135

  Fragments arranged by size: 

                 648     336     168     168     135      84      84      84
                  84      84      84
 
AflIII A'CryG_T

Cuts at:      0     722     890     974    1646    1825    1959 
   Size:        722     168      84     672     179     134

  Fragments arranged by size: 

                 722     672     179     168     134      84
 
AleI CACnn'nnGTG

Cuts at:      0     875    1959 
   Size:        875    1084
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1070    1959 
   Size:       1070     889
 
ApaI G_GGCC'C

Cuts at:      0     367    1959 
   Size:        367    1592
 
ApoI r'AATT_y

Cuts at:      0     122     274    1260    1959 
   Size:        122     152     986     699

  Fragments arranged by size: 

                 986     699     152     122
 
AvaI C'yCGr_G

Cuts at:      0     789    1629    1959 
   Size:        789     840     330

  Fragments arranged by size: 

                 840     789     330
 
BamHI G'GATC_C

Cuts at:      0     357    1959 
   Size:        357    1602
 
BanI G'GyrC_C

Cuts at:      0     445    1959 
   Size:        445    1514
 
BanII G_rGCy'C

Cuts at:      0      58     233     367    1710    1959 
   Size:         58     175     134    1343     249

  Fragments arranged by size: 

                1343     249     175     134      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1057    1879    1959 
   Size:       1057     822      80

  Fragments arranged by size: 

                1057     822      80
 
BbvCI CC'TCA_GC

Cuts at:      0    1858    1959 
   Size:       1858     101
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     692    1959 
   Size:        692    1267
 
BclI T'GATC_A

Cuts at:      0     417    1959 
   Size:        417    1542
 
BlpI GC'TnA_GC

Cuts at:      0      52    1959 
   Size:         52    1907
 
Bme1580I G_kGCm'C

Cuts at:      0     367    1247    1959 
   Size:        367     880     712

  Fragments arranged by size: 

                 880     712     367
 
BmgBI CAC'GTC

Cuts at:      0    1826    1880    1959 
   Size:       1826      54      79

  Fragments arranged by size: 

                1826      79      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     127    1304    1675    1959 
   Size:        127    1177     371     284

  Fragments arranged by size: 

                1177     371     284     127
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      77    1959 
   Size:         77    1882
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     178     198     249     432     480    1959 
   Size:        112      66      20      51     183      48    1479

  Fragments arranged by size: 

                1479     183     112      66      51      48      20
 
Bpu10I CC'TnA_GC

Cuts at:      0    1858    1916    1959 
   Size:       1858      58      43

  Fragments arranged by size: 

                1858      58      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1327    1831    1959 
   Size:       1327     504     128

  Fragments arranged by size: 

                1327     504     128
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     117     166     399    1609    1959 
   Size:        117      49     233    1210     350

  Fragments arranged by size: 

                1210     350     233     117      49
 
BsaAI yAC'GTr

Cuts at:      0     723     975    1959 
   Size:        723     252     984

  Fragments arranged by size: 

                 984     723     252
 
BsaWI w'CCGG_w

Cuts at:      0     836     874    1959 
   Size:        836      38    1085

  Fragments arranged by size: 

                1085     836      38
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1090    1120    1291    1321    1543    1573    1959 
   Size:       1090      30     171      30     222      30     386

  Fragments arranged by size: 

                1090     386     222     171      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1060    1959 
   Size:       1060     899
 
BseYI C'CCAG_C

Cuts at:      0     152     564    1959 
   Size:        152     412    1395

  Fragments arranged by size: 

                1395     412     152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     831     915    1083    1671    1755    1924    1946 
   Size:        353     478      84     168     588      84     169      22

Cuts at:   1946    1959 
   Size:         13

  Fragments arranged by size: 

                 588     478     353     169     168      84      84      22
                  13
 
BsiHKAI G_wGCw'C

Cuts at:      0     233    1959 
   Size:        233    1726
 
BsmI GAATG_Cn'

Cuts at:      0       4    1959 
   Size:          4    1955
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     233     367    1247    1710    1959 
   Size:         58     175     134     880     463     249

  Fragments arranged by size: 

                 880     463     249     175     134      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     689    1959 
   Size:        689    1270
 
BsrFI r'CCGG_y

Cuts at:      0     201    1600    1959 
   Size:        201    1399     359

  Fragments arranged by size: 

                1399     359     201
 
BsrGI T'GTAC_A

Cuts at:      0      74    1959 
   Size:         74    1885
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     291    1959 
   Size:        291    1668
 
BstYI r'GATC_y

Cuts at:      0     216     314     357    1959 
   Size:        216      98      43    1602

  Fragments arranged by size: 

                1602     216      98      43
 
Bsu36I CC'TnA_GG

Cuts at:      0     434     584    1959 
   Size:        434     150    1375

  Fragments arranged by size: 

                1375     434     150
 
BtgI C'CryG_G

Cuts at:      0    1475    1959 
   Size:       1475     484
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1176    1959 
   Size:       1176     783
 
BtsI GCAGTG_nn'

Cuts at:      0     903    1071    1663    1959 
   Size:        903     168     592     296

  Fragments arranged by size: 

                 903     592     296     168
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1137    1172    1389    1424    1809    1844    1959 
   Size:       1137      35     217      35     385      35     115

  Fragments arranged by size: 

                1137     385     217     115      35      35      35
 
EaeI y'GGCC_r

Cuts at:      0    1410    1746    1959 
   Size:       1410     336     213

  Fragments arranged by size: 

                1410     336     213
 
EarI CTCTTCn'nnn_

Cuts at:      0     601     754    1712    1959 
   Size:        601     153     958     247

  Fragments arranged by size: 

                 958     601     247     153
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1299    1959 
   Size:       1299     660
 
EcoICRI GAG'CTC

Cuts at:      0     231    1959 
   Size:        231    1728
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     178     198     249     432     480     648     732 
   Size:        112      66      20      51     183      48     168      84

Cuts at:    732     900     984    1068    1152    1236    1404    1488    1824 
   Size:        168      84      84      84      84     168      84     336

Cuts at:   1824    1959 
   Size:        135

  Fragments arranged by size: 

                 336     183     168     168     168     135     112      84
                  84      84      84      84      84      66      51      48
                  20
 
EcoO109I rG'GnC_Cy

Cuts at:      0     430    1959 
   Size:        430    1529
 
EcoRI G'AATT_C

Cuts at:      0    1260    1959 
   Size:       1260     699
 
HincII GTy'rAC

Cuts at:      0     639     845    1191    1959 
   Size:        639     206     346     768

  Fragments arranged by size: 

                 768     639     346     206
 
MfeI C'AATT_G

Cuts at:      0     803    1959 
   Size:        803    1156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1457    1959 
   Size:       1457     502
 
MscI TGG'CCA

Cuts at:      0    1748    1959 
   Size:       1748     211
 
MslI CAynn'nnrTG

Cuts at:      0     327     875    1815    1959 
   Size:        327     548     940     144

  Fragments arranged by size: 

                 940     548     327     144
 
MspA1I CmG'CkG

Cuts at:      0     156     696    1015    1959 
   Size:        156     540     319     944

  Fragments arranged by size: 

                 944     540     319     156
 
NcoI C'CATG_G

Cuts at:      0    1475    1959 
   Size:       1475     484
 
NdeI CA'TA_TG

Cuts at:      0    1812    1959 
   Size:       1812     147
 
NspI r_CATG'y

Cuts at:      0     292     326     894    1650    1959 
   Size:        292      34     568     756     309

  Fragments arranged by size: 

                 756     568     309     292      34
 
PasI CC'CwG_GG

Cuts at:      0     367    1959 
   Size:        367    1592
 
PciI A'CATG_T

Cuts at:      0     890    1646    1959 
   Size:        890     756     313

  Fragments arranged by size: 

                 890     756     313
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1360    1392    1959 
   Size:       1360      32     567

  Fragments arranged by size: 

                1360     567      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     430    1959 
   Size:        430    1529
 
PspOMI G'GGCC_C

Cuts at:      0     363    1959 
   Size:        363    1596
 
PspXI vC'TCGA_Gb

Cuts at:      0    1629    1959 
   Size:       1629     330
 
PvuII CAG'CTG

Cuts at:      0     156    1015    1959 
   Size:        156     859     944

  Fragments arranged by size: 

                 944     859     156
 
SacI G_AGCT'C

Cuts at:      0     233    1959 
   Size:        233    1726
 
SapI GCTCTTCn'nnn_

Cuts at:      0     754    1959 
   Size:        754    1205
 
SexAI A'CCwGG_T

Cuts at:      0     107    1959 
   Size:        107    1852
 
SfcI C'TryA_G

Cuts at:      0      99    1959 
   Size:         99    1860
 
SmlI C'TyrA_G

Cuts at:      0    1342    1629    1846    1959 
   Size:       1342     287     217     113

  Fragments arranged by size: 

                1342     287     217     113
 
StyI C'CwwG_G

Cuts at:      0    1475    1749    1959 
   Size:       1475     274     210

  Fragments arranged by size: 

                1475     274     210
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      46     166    1959 
   Size:         46     120    1793

  Fragments arranged by size: 

                1793     120      46
 
TatI w'GTAC_w

Cuts at:      0      74    1959 
   Size:         74    1885
 
Tth111I GACn'n_nGTC

Cuts at:      0    1878    1959 
   Size:       1878      81
 
XhoI C'TCGA_G

Cuts at:      0    1629    1959 
   Size:       1629     330
 
XmnI GAAnn'nnTTC

Cuts at:      0     745    1959 
   Size:        745    1214
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrFI    BsrGI   BstAPI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI   HincII     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NspI     PasI     PciI     PpiI    PpuMI   PspOMI    PspXI
    PvuII     SacI     SapI    SexAI     SfcI     SmlI     StyI    TaqII
     TatI  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BfrBI     BglI    BglII     BmtI    BsaBI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I     ClaI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI     XbaI     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com