Restriction map for pF1KB5280
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BmyI        BpuAI       BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          32  CGCG                 CG^CG
AlwI          555  GATCC                GGATC(4/5)
Aor51HI        53  AGCGCT               AGC^GCT
AvaII           6  GGACC                G^G[AT]CC
BanI          160  GGTGCC               G^G[CT][GA]CC
BglI           40  GCCGAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          616  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      616  GCTGAGC              GC^T[ACGT]AGC
BsaBI         121  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         209  CCATGG               C^C[ACGT][ACGT]GG
BseRI         130  CTCCTC               GAGGAG(10/8)
BsiYI         132  CCTCTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          132  CCTCTCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI           5  GGGAC                GGGAC(10/14)
BsmI          561  GAATGC               GAATGC(1/-1)
BsrI          345  CCAGT                ACTGG(1/-1)
BstUI          32  CGCG                 CG^CG
BstXI         509  CCATCCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         554  AGATCC               [GA]^GATC[CT]
CelII         616  GCTGAGC              GC^T[ACGT]AGC
DsaI          209  CCATGG               C^C[GA][CT]GG
EaeI           38  TGGCCG               [CT]^GGCC[GA]
Eco47III       53  AGCGCT               AGC^GCT
EcoT14I       209  CCATGG               C^C[AT][AT]GG
FauI           27  GCGGG                CCCGC(4/6)
HaeII          53  AGCGCT               [GA]GCGC^[CT]
MaeIII         88  GTGAC                ^GT[ACGT]AC
MamI          121  GATCGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          554  AGATCC               [GA]^GATC[CT]
MseI          245  TTAA                 T^TAA
MvnI           32  CGCG                 CG^CG
NcoI          209  CCATGG               C^CATGG
PleI          652  GACTC                GAGTC(4/5)
PstI           22  CTGCAG               CTGCA^G
PvuII          16  CAGCTG               CAG^CTG
SfcI           22  CTGCAG               C^T[GA][CT]AG
StyI          209  CCATGG               C^C[AT][AT]GG
TfiI          640  GATTC                G^A[AT]TC
Tru9I         245  TTAA                 T^TAA
Tsp45I         88  GTGAC                ^GT[GC]AC
Tsp509I       233  AATT                 ^AATT
TspRI         432  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          611  CCATAGCTGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         554  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AlwNI          16  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              670  CAGTTGCTG           
BclI          323  TGATCA               T^GATCA
              662  TGATCA              
BcnI            9  CCGGG                CC^[GC]GG
              255  CCGGG               
BpmI          177  CTGGAG               CTGGAG(16/14)
              277  CTGGAG              
BsrDI         290  GCAATG               GCAATG(2/0)
              335  GCAATG              
BstAPI         15  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              669  GCAGTTGCTGC         
CfoI           31  GCGC                 GCG^C
               54  GCGC                
FbaI          323  TGATCA               T^GATCA
              662  TGATCA              
HhaI           31  GCGC                 GCG^C
               54  GCGC                
HinP1I         31  GCGC                 G^CGC
               54  GCGC                
HindIII       248  AAGCTT               A^AGCTT
              443  AAGCTT              
HinfI         640  GATTC                G^A[ACGT]TC
              652  GACTC               
MspA1I         16  CAGCTG               C[AC]G^C[GT]G
               25  CAGCGG              
NciI            9  CCGGG                CC^[GC]GG
              255  CCGGG               
NlaIV           5  GGGACC               GG[ACGT]^[ACGT]CC
              160  GGTGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BbvI        BsmAI       BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TseI        TthHB8I     
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