Restriction map for pF1KB5244
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmFI       BsmI        BspDI       
BspEI       BspLU11I    BsrDI       BsrFI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KspI        MaeIII      MfeI        MluI        MroI        
MslI        MunI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        TfiI        
Tsp45I      Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        694  GGTACC               G^GTACC
AlwNI         403  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          929  GGGCCC               GGGCC^C
Asp718I       694  GGTACC               G^GTACC
AspHI          97  GAGCAC               G[AT]GC[AT]^C
AspI          490  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          323  CCCGGG               C^[CT]CG[GA]G
AviII         371  TGCGCA               TGC^GCA
BalI          434  TGGCCA               TGG^CCA
BbsI          715  GTCTTC               GAAGAC(2/6)
BfaI          361  CTAG                 C^TAG
BlpI          828  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      828  GCTCAGC              GC^T[ACGT]AGC
BpuAI         715  GTCTTC               GAAGAC(2/6)
BsaBI         226  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          495  GGTCTC               GGTCTC(1/5)
BsiHKAI        97  GAGCAC               G[AT]GC[AT]^C
BsoBI         323  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      645  TGTACA               T^GTACA
BsrGI         645  TGTACA               T^GTACA
BssSI         100  CACGAG               CACGAG(-5/-1)
BstF5I        225  GGATG                GGATG(2/0)
CelII         828  GCTCAGC              GC^T[ACGT]AGC
DraI          295  TTTAAA               TTT^AAA
FokI          225  GGATG                GGATG(9/13)
FspI          371  TGCGCA               TGC^GCA
HapII         324  CCGG                 C^CGG
HpaII         324  CCGG                 C^CGG
KpnI          694  GGTACC               GGTAC^C
MaeI          361  CTAG                 C^TAG
MaeII         722  ACGT                 A^CGT
MamI          226  GATGACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         434  TGGCCA               TGG^CCA
MscI          434  TGGCCA               TGG^CCA
MspA1I        962  CCGCTG               C[AC]G^C[GT]G
MspI          324  CCGG                 C^CGG
NcoI          698  CCATGG               C^CATGG
PflFI         490  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI          70  GACGAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          911  CTGCAG               CTGCA^G
SfaNI         833  GCATC                GCATC(5/9)
SmaI          323  CCCGGG               CCC^GGG
SphI          338  GCATGC               GCATG^C
SspBI         645  TGTACA               T^GTACA
SspI          994  AATATT               AAT^ATT
Tth111I       490  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          536  CCACCACCAACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         323  CCCGGG               C^CCGGG
XmaI          323  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         250  CGCG                 CG^CG
              354  CGCG                
Aor51HI       631  AGCGCT               AGC^GCT
              753  AGCGCT              
BbvCI          40  CCTCAGC              CCTCAGC(-5/-2)
              816  CCTCAGC             
BcnI          323  CCCGG                CC^[GC]GG
              324  CCGGG               
BpmI          286  CTGGAG               CTGGAG(16/14)
              757  CTGGAG              
BsmBI         246  GAGACG               CGTCTC(1/5)
              303  GAGACG              
BspMI         368  ACCTGC               ACCTGC(4/8)
              617  ACCTGC              
BsrBI         826  CCGCTC               CCGCTC(-3/-3)
              328  GAGCGG              
BstUI         250  CGCG                 CG^CG
              354  CGCG                
BstXI         539  CCACCAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              663  CCAGTCGCCTGG        
BstYI         464  AGATCC               [GA]^GATC[CT]
              980  AGATCC              
DsaI          698  CCATGG               C^C[GA][CT]GG
              939  CCACGG              
EaeI          434  TGGCCA               [CT]^GGCC[GA]
              626  CGGCCA              
Eco47III      631  AGCGCT               AGC^GCT
              753  AGCGCT              
EcoNI         195  CCTGGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              369  CCTGCGCAAGG         
EcoT14I        48  CCAAGG               C^C[AT][AT]GG
              698  CCATGG              
FauI          822  CCCGC                CCCGC(4/6)
              553  GCGGG               
MflI          464  AGATCC               [GA]^GATC[CT]
              980  AGATCC              
MseI          296  TTAA                 T^TAA
              725  TTAA                
MvnI          250  CGCG                 CG^CG
              354  CGCG                
NciI          323  CCCGG                CC^[GC]GG
              324  CCGGG               
NspI          338  GCATGC               [GA]CATG^[CT]
              605  ACATGC              
PpuMI          51  AGGTCCT              [GA]G^G[AT]CC[CT]
              191  AGGACCT             
SexAI         491  ACCTGGT              A^CC[AT]GGT
              545  ACCTGGT             
SfcI          744  CTACAG               C^T[GA][CT]AG
              911  CTGCAG              
StyI           48  CCAAGG               C^C[AT][AT]GG
              698  CCATGG              
Tru9I         296  TTAA                 T^TAA
              725  TTAA                
Tsp509I       299  AATT                 ^AATT
              886  AATT                
TspRI         155  CACTG                CA[GC]TG[ACGT][ACGT]^
              920  CACTG               
XhoII         464  AGATCC               [GA]^GATC[CT]
              980  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BanI        BanII       BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       Bsp1286I    
BspHI       BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EarI        EcoO109I    EcoRII      Fnu4HI      HaeII       
HaeIII      HgaI        HhaI        HinP1I      HinfI       
ItaI        Ksp632I     MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       TaqI        TseI        TthHB8I     
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