Restriction map for pF1KB5244
 (Linear) (Six-base) MAPSORT of: KIBB5244.seq  Check: 9759  from: 1  to: 1068



KIBB5244.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     626    1068 
   Size:        626     442
 
Acc65I G'GTAC_C

Cuts at:      0     694    1068 
   Size:        694     374
 
AfeI AGC'GCT

Cuts at:      0     633     755    1068 
   Size:        633     122     313

  Fragments arranged by size: 

                 633     313     122
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     926    1068 
   Size:        397     529     142

  Fragments arranged by size: 

                 529     397     142
 
AlwNI CAG_nnn'CTG

Cuts at:      0     408    1068 
   Size:        408     660
 
ApaI G_GGCC'C

Cuts at:      0     933    1068 
   Size:        933     135
 
AvaI C'yCGr_G

Cuts at:      0     323    1068 
   Size:        323     745
 
BanI G'GyrC_C

Cuts at:      0     381     694     958    1068 
   Size:        381     313     264     110

  Fragments arranged by size: 

                 381     313     264     110
 
BanII G_rGCy'C

Cuts at:      0     456     764     933    1068 
   Size:        456     308     169     135

  Fragments arranged by size: 

                 456     308     169     135
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     708    1068 
   Size:        708     360
 
BbvCI CC'TCA_GC

Cuts at:      0      41     817    1068 
   Size:         41     776     251

  Fragments arranged by size: 

                 776     251      41
 
BlpI GC'TnA_GC

Cuts at:      0     829    1068 
   Size:        829     239
 
Bme1580I G_kGCm'C

Cuts at:      0     933    1044    1068 
   Size:        933     111      24

  Fragments arranged by size: 

                 933     111      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     674    1068 
   Size:        674     394
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168    1068 
   Size:        168     900
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     778    1068 
   Size:        307     471     290

  Fragments arranged by size: 

                 471     307     290
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     474     590     817    1068 
   Size:         41     433     116     227     251

  Fragments arranged by size: 

                 433     251     227     116      41
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     501    1068 
   Size:        501     567
 
BsaBI GATnn'nnATC

Cuts at:      0     230    1068 
   Size:        230     838
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     481     511     640     670    1068 
   Size:        481      30     129      30     398

  Fragments arranged by size: 

                 481     398     129      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     160     187     190     217     235    1068 
   Size:        109      51      27       3      27      18     833

  Fragments arranged by size: 

                 833     109      51      27      27      18       3
 
BseYI C'CCAG_C

Cuts at:      0    1043    1068 
   Size:       1043      25
 
BsiHKAI G_wGCw'C

Cuts at:      0     101    1068 
   Size:        101     967
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     240     297    1068 
   Size:        240      57     771

  Fragments arranged by size: 

                 771     240      57
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     456     764     933    1044    1068 
   Size:        101     355     308     169     111      24

  Fragments arranged by size: 

                 355     308     169     111     101      24
 
BspHI T'CATG_A

Cuts at:      0    1023    1034    1062    1068 
   Size:       1023      11      28       6

  Fragments arranged by size: 

                1023      28      11       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     377     626    1068 
   Size:        377     249     442

  Fragments arranged by size: 

                 442     377     249
 
BsrBI CCG'CTC

Cuts at:      0     330     828    1068 
   Size:        330     498     240

  Fragments arranged by size: 

                 498     330     240
 
BsrGI T'GTAC_A

Cuts at:      0     645    1068 
   Size:        645     423
 
BssSI C'ACGA_G

Cuts at:      0     100    1068 
   Size:        100     968
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     546     670    1068 
   Size:        546     124     398

  Fragments arranged by size: 

                 546     398     124
 
BstYI r'GATC_y

Cuts at:      0     464     980    1068 
   Size:        464     516      88

  Fragments arranged by size: 

                 516     464      88
 
BtgI C'CryG_G

Cuts at:      0     698     939    1068 
   Size:        698     241     129

  Fragments arranged by size: 

                 698     241     129
 
DraI TTT'AAA

Cuts at:      0     297    1068 
   Size:        297     771
 
EaeI y'GGCC_r

Cuts at:      0     434     626    1068 
   Size:        434     192     442

  Fragments arranged by size: 

                 442     434     192
 
EarI CTCTTCn'nnn_

Cuts at:      0     194     206     212    1068 
   Size:        194      12       6     856

  Fragments arranged by size: 

                 856     194      12       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     674     722     858    1068 
   Size:        674      48     136     210

  Fragments arranged by size: 

                 674     210     136      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     778    1068 
   Size:        307     471     290

  Fragments arranged by size: 

                 471     307     290
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     199     373    1068 
   Size:        199     174     695

  Fragments arranged by size: 

                 695     199     174
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52     192     929    1068 
   Size:         52     140     737     139

  Fragments arranged by size: 

                 737     140     139      52
 
FspI TGC'GCA

Cuts at:      0     373    1068 
   Size:        373     695
 
HaeII r_GCGC'y

Cuts at:      0     361     635     757    1068 
   Size:        361     274     122     311

  Fragments arranged by size: 

                 361     311     274     122
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     264     296     481     513    1068 
   Size:        264      32     185      32     555

  Fragments arranged by size: 

                 555     264     185      32      32
 
KpnI G_GTAC'C

Cuts at:      0     698    1068 
   Size:        698     370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     440    1068 
   Size:        440     628
 
MscI TGG'CCA

Cuts at:      0     436    1068 
   Size:        436     632
 
MspA1I CmG'CkG

Cuts at:      0     964    1068 
   Size:        964     104
 
NcoI C'CATG_G

Cuts at:      0     698    1068 
   Size:        698     370
 
NspI r_CATG'y

Cuts at:      0     342     609    1068 
   Size:        342     267     459

  Fragments arranged by size: 

                 459     342     267
 
PpuMI rG'GwC_Cy

Cuts at:      0      52     192    1068 
   Size:         52     140     876

  Fragments arranged by size: 

                 876     140      52
 
PshAI GACnn'nnGTC

Cuts at:      0      74    1068 
   Size:         74     994
 
PspOMI G'GGCC_C

Cuts at:      0     929    1068 
   Size:        929     139
 
PstI C_TGCA'G

Cuts at:      0     915    1068 
   Size:        915     153
 
SexAI A'CCwGG_T

Cuts at:      0     491     545    1068 
   Size:        491      54     523

  Fragments arranged by size: 

                 523     491      54
 
SfcI C'TryA_G

Cuts at:      0     744     911    1068 
   Size:        744     167     157

  Fragments arranged by size: 

                 744     167     157
 
SmaI CCC'GGG

Cuts at:      0     325    1068 
   Size:        325     743
 
SphI G_CATG'C

Cuts at:      0     342    1068 
   Size:        342     726
 
SspI AAT'ATT

Cuts at:      0     996    1068 
   Size:        996      72
 
StyI C'CwwG_G

Cuts at:      0      48     698    1068 
   Size:         48     650     370

  Fragments arranged by size: 

                 650     370      48
 
TatI w'GTAC_w

Cuts at:      0     645    1068 
   Size:        645     423
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     640     672     850     882    1068 
   Size:        640      32     178      32     186

  Fragments arranged by size: 

                 640     186     178      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     493    1068 
   Size:        493     575
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     543    1068 
   Size:        543     525
 
XmaI C'CCGG_G

Cuts at:      0     323    1068 
   Size:        323     745
 

 Enzymes that do cut:

     AarI   Acc65I     AfeI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI    BbvCI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrGI    BssSI    BstXI    BstYI
     BtgI     DraI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FspI    HaeII    Hin4I     KpnI     MmeI     MscI   MspA1I     NcoI
     NspI    PpuMI    PshAI   PspOMI     PstI    SexAI     SfcI     SmaI
     SphI     SspI     StyI     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII    BmgBI     BmtI    BpuEI    BsaAI    BsaHI    BsaWI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BsrDI    BsrFI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII
     XbaI     XhoI     XmnI     ZraI
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