Restriction map for pF1KB5242
##################################
# NO CUT ENZYMES (  118 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
BalI        BbeI        BbsI        BcgI        BclI        
BfrI        BglII       BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        Hin1I       HindIII     
HpaI        KasI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PmeI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AflIII        476  ACGTGT               A^C[GA][CT]GT
AgeI          668  ACCGGT               A^CCGGT
AviII          61  TGCGCA               TGC^GCA
AvrII        1431  CCTAGG               C^CTAGG
BamHI         824  GGATCC               G^GATCC
BbvCI        1135  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1030  GGATAC               GTATCC(6/5)
BlnI         1431  CCTAGG               C^CTAGG
BsaWI         668  ACCGGT               [AT]^CCGG[AT]
BsrBI        1423  GAGCGG               CCGCTC(-3/-3)
BsrDI         699  CATTGC               GCAATG(2/0)
BssHII        327  GCGCGC               G^CGCGC
BstYI         824  GGATCC               [GA]^GATC[CT]
EarI          276  GAAGAG               CTCTTC(1/4)
FspI           61  TGCGCA               TGC^GCA
HgaI          981  GCGTC                GACGC(5/10)
HincII        865  GTCAAC               GT[CT]^[GA]AC
HindII        865  GTCAAC               GT[CT]^[GA]AC
Ksp632I       276  GAAGAG               CTCTTC(1/4)
KspI           10  CCGCGG               CCGC^GG
MboII         276  GAAGA                GAAGA(8/7)
MflI          824  GGATCC               [GA]^GATC[CT]
PinAI         668  ACCGGT               A^CCGGT
PshAI         421  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1150  CAGCTG               CAG^CTG
SacI         1021  GAGCTC               GAGCT^C
SacII          10  CCGCGG               CCGC^GG
SapI          276  GAAGAGC              GCTCTTC(1/4)
XhoII         824  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccII          11  CGCG                 CG^CG
              328  CGCG                
AocI         1416  CCTGAGG              CC^T[ACGT]AGG
             1515  CCTGAGG             
AspHI         640  GTGCTC               G[AT]GC[AT]^C
             1021  GAGCTC              
BbrPI         475  CACGTG               CAC^GTG
              631  CACGTG              
BfaI         1316  CTAG                 C^TAG
             1432  CTAG                
BsaAI         475  CACGTG               [CT]AC^GT[GA]
              631  CACGTG              
BsiHKAI       640  GTGCTC               G[AT]GC[AT]^C
             1021  GAGCTC              
BsrFI         209  ACCGGC               [GA]^CCGG[CT]
              668  ACCGGT              
BstF5I        438  CATCC                GGATG(2/0)
              471  CATCC               
BstUI          11  CGCG                 CG^CG
              328  CGCG                
Bsu36I       1416  CCTGAGG              CC^T[ACGT]AGG
             1515  CCTGAGG             
Cfr10I        209  ACCGGC               [GA]^CCGG[CT]
              668  ACCGGT              
DpnII         162  GATC                 ^GATC
              825  GATC                
EagI         1007  CGGCCG               C^GGCCG
             1389  CGGCCG              
EclXI        1007  CGGCCG               C^GGCCG
             1389  CGGCCG              
Eco52I       1007  CGGCCG               C^GGCCG
             1389  CGGCCG              
Eco81I       1416  CCTGAGG              CC^T[ACGT]AGG
             1515  CCTGAGG             
EcoT14I      1285  CCAAGG               C^C[AT][AT]GG
             1431  CCTAGG              
FokI          438  CATCC                GGATG(9/13)
              471  CATCC               
HaeII         348  GGCGCT               [GA]GCGC^[CT]
             1318  AGCGCC              
HphI           91  TCACC                GGTGA(8/7)
             1091  TCACC               
MaeI         1316  CTAG                 C^TAG
             1432  CTAG                
MaeII         476  ACGT                 A^CGT
              632  ACGT                
MboI          162  GATC                 ^GATC
              825  GATC                
MvnI           11  CGCG                 CG^CG
              328  CGCG                
NdeII         162  GATC                 ^GATC
              825  GATC                
PaeR7I       1328  CTCGAG               C^TCGAG
             1407  CTCGAG              
PmaCI         475  CACGTG               CAC^GTG
              631  CACGTG              
PmlI          475  CACGTG               CAC^GTG
              631  CACGTG              
PstI          253  CTGCAG               CTGCA^G
              415  CTGCAG              
Sau3AI        162  GATC                 ^GATC
              825  GATC                
SmaI           29  CCCGGG               CCC^GGG
               70  CCCGGG              
StuI         1434  AGGCCT               AGG^CCT
             1491  AGGCCT              
StyI         1285  CCAAGG               C^C[AT][AT]GG
             1431  CCTAGG              
XhoI         1328  CTCGAG               C^TCGAG
             1407  CTCGAG              
XmaCI          29  CCCGGG               C^CCGGG
               70  CCCGGG              
XmaI           29  CCCGGG               C^CCGGG
               70  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BstAPI      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeIII      MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NlaIII      NlaIV       
PflMI       PleI        PpuMI       RsaI        Sau96I      
ScrFI       SfcI        SmlI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      
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