Restriction map for pF1KB5231
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BclI        BfrI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstYI       
BstZ17I     CelII       CfoI        Cfr10I      CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MroI        MslI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AccI         1843  GTAGAC               GT^[AC][GT]AC
AccII          12  CGCG                 CG^CG
AlwI         1684  GGATC                GGATC(4/5)
AocI         1939  CCTAAGG              CC^T[ACGT]AGG
Asp700I      1498  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          851  TGGCCA               TGG^CCA
BcgI          984  CGATGTTTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1743  GGATAC               GTATCC(6/5)
BsmI          414  GAATGC               GAATGC(1/-1)
BspDI        1360  ATCGAT               AT^CGAT
BsrDI        1118  GCAATG               GCAATG(2/0)
BstBI        1597  TTCGAA               TT^CGAA
BstUI          12  CGCG                 CG^CG
Bsu36I       1939  CCTAAGG              CC^T[ACGT]AGG
ClaI         1360  ATCGAT               AT^CGAT
DraI         1617  TTTAAA               TTT^AAA
DsaI         1886  CCATGG               C^C[GA][CT]GG
EaeI          851  TGGCCA               [CT]^GGCC[GA]
Eco81I       1939  CCTAAGG              CC^T[ACGT]AGG
EcoNI        1734  CCTGTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1574  ATGCAT               ATGCA^T
HgaI         1603  GACGC                GACGC(5/10)
HindIII      1613  AAGCTT               A^AGCTT
MaeII         692  ACGT                 A^CGT
MluNI         851  TGGCCA               TGG^CCA
MscI          851  TGGCCA               TGG^CCA
MvnI           12  CGCG                 CG^CG
NcoI         1886  CCATGG               C^CATGG
NlaIII       1887  CATG                 CATG^
NruI           11  TCGCGA               TCG^CGA
NsiI         1574  ATGCAT               ATGCA^T
NspV         1597  TTCGAA               TT^CGAA
SapI          808  GAAGAGC              GCTCTTC(1/4)
SfuI         1597  TTCGAA               TT^CGAA
StuI         1731  AGGCCT               AGG^CCT
XmnI         1498  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI         1249  GCGG                 CCGC(-3/-1)
             1982  GCGG                
AfaI         1383  GTAC                 GT^AC
             1540  GTAC                
AvaII        1675  GGACC                G^G[AT]CC
             1820  GGTCC               
BbvCI         176  CCTCAGC              CCTCAGC(-5/-2)
              697  GCTGAGG             
BcnI         1809  CCGGG                CC^[GC]GG
             2040  CCGGG               
BfaI         1037  CTAG                 C^TAG
             1132  CTAG                
BsmAI         634  GAGAC                GTCTC(1/5)
             1756  GAGAC               
BspMI         276  ACCTGC               ACCTGC(4/8)
               19  GCAGGT              
BstEII         34  GGTGACC              G^GT[ACGT]ACC
             2092  GGTGACC             
BstPI          34  GGTGACC              G^GT[ACGT]ACC
             2092  GGTGACC             
BstXI          41  CCAAGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              686  CCAAGAACGTGG        
Cac8I           9  GCTCGC               GC[ACGT]^[ACGT]GC
             1703  GCCAGC              
DraII        1674  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1819  GGGTCCT             
EcoO109I     1674  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1819  GGGTCCT             
EcoO65I        34  GGTGACC              G^GT[ACGT]ACC
             2092  GGTGACC             
HapII        1809  CCGG                 C^CGG
             2040  CCGG                
HpaII        1809  CCGG                 C^CGG
             2040  CCGG                
MaeI         1037  CTAG                 C^TAG
             1132  CTAG                
MspA1I        242  CAGCTG               C[AC]G^C[GT]G
              533  CAGCTG              
MspI         1809  CCGG                 C^CGG
             2040  CCGG                
NciI         1809  CCGGG                CC^[GC]GG
             2040  CCGGG               
PleI          613  GACTC                GAGTC(4/5)
             1768  GAGTC               
PpuMI        1674  AGGACCC              [GA]G^G[AT]CC[CT]
             1819  GGGTCCT             
PvuII         242  CAGCTG               CAG^CTG
              533  CAGCTG              
RsaI         1383  GTAC                 GT^AC
             1540  GTAC                
SfcI         1416  CTATAG               C^T[GA][CT]AG
             1482  CTACAG              
TaqI         1361  TCGA                 T^CGA
             1598  TCGA                
TfiI         1063  GAATC                G^A[AT]TC
             1450  GAATC               
TthHB8I      1361  TCGA                 T^CGA
             1598  TCGA                
XcmI         1545  CCAGAACCAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1877  CCAAGGAGGCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AcsI        AluI        AlwNI       ApoI        BbsI        
BbvI        BglI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com