Restriction map for pF1KB5231
 (Linear) (Six-base) MAPSORT of: KIBB5231.seq  Check: 8586  from: 1  to: 2130



KIBB5231.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     285    2130 
   Size:        285    1845
 
AccI GT'mk_AC

Cuts at:      0    1844    2130 
   Size:       1844     286
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638     893    1091    1489    1772    2130 
   Size:        638     255     198     398     283     358

  Fragments arranged by size: 

                 638     398     358     283     255     198
 
AlwNI CAG_nnn'CTG

Cuts at:      0     535     538     871    1318    2130 
   Size:        535       3     333     447     812

  Fragments arranged by size: 

                 812     535     447     333       3
 
ApoI r'AATT_y

Cuts at:      0    1042    1303    1620    2130 
   Size:       1042     261     317     510

  Fragments arranged by size: 

                1042     510     317     261
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     292     434    1079    1607    1898    2122    2130 
   Size:        292     142     645     528     291     224       8

  Fragments arranged by size: 

                 645     528     292     291     224     142       8
 
BbvCI CC'TCA_GC

Cuts at:      0     177     698    2130 
   Size:        177     521    1432

  Fragments arranged by size: 

                1432     521     177
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     973    1007    2130 
   Size:        973      34    1123

  Fragments arranged by size: 

                1123     973      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1737    2130 
   Size:       1737     393
 
BfrBI ATG'CAT

Cuts at:      0    1576    2130 
   Size:       1576     554
 
BglI GCCn_nnn'nGGC

Cuts at:      0     397    1882    2045    2130 
   Size:        397    1485     163      85

  Fragments arranged by size: 

                1485     397     163      85
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1826    1938    2130 
   Size:       1826     112     192

  Fragments arranged by size: 

                1826     192     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236    1099    1412    1684    2130 
   Size:        236     863     313     272     446

  Fragments arranged by size: 

                 863     446     313     272     236
 
Bpu10I CC'TnA_GC

Cuts at:      0     177     698    2130 
   Size:        177     521    1432

  Fragments arranged by size: 

                1432     521     177
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     119     149    1072    1102    2130 
   Size:        119      30     923      30    1028

  Fragments arranged by size: 

                1028     923     119      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      44      47     457     475     484     799     802     814 
   Size:         44       3     410      18       9     315       3      12

Cuts at:    814     817    1873    2024    2033    2075    2087    2096    2099 
   Size:          3    1056     151       9      42      12       9       3

Cuts at:   2099    2102    2130 
   Size:          3      28

  Fragments arranged by size: 

                1056     410     315     151      44      42      28      18
                  12      12       9       9       9       3       3       3
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     387    2130 
   Size:        387    1743
 
BsmI GAATG_Cn'

Cuts at:      0     420    2130 
   Size:        420    1710
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10     285    2130 
   Size:         10     275    1845

  Fragments arranged by size: 

                1845     275      10
 
BsrDI GCAATG_nn'

Cuts at:      0    1125    2130 
   Size:       1125    1005
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     277     535     538    2130 
   Size:        277     258       3    1592

  Fragments arranged by size: 

                1592     277     258       3
 
BstBI TT'CG_AA

Cuts at:      0    1598    2130 
   Size:       1598     532
 
BstEII G'GTnAC_C

Cuts at:      0      34    2092    2130 
   Size:         34    2058      38

  Fragments arranged by size: 

                2058      38      34
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     693    2130 
   Size:         48     645    1437

  Fragments arranged by size: 

                1437     645      48
 
Bsu36I CC'TnA_GG

Cuts at:      0    1940    2130 
   Size:       1940     190
 
BtgI C'CryG_G

Cuts at:      0    1886    2130 
   Size:       1886     244
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     535     998    2130 
   Size:        535     463    1132

  Fragments arranged by size: 

                1132     535     463
 
BtsI GCAGTG_nn'

Cuts at:      0     129    2130 
   Size:        129    2001
 
ClaI AT'CG_AT

Cuts at:      0    1361    2130 
   Size:       1361     769
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     170     205    2130 
   Size:        170      35    1925

  Fragments arranged by size: 

                1925     170      35
 
DraI TTT'AAA

Cuts at:      0    1619    2130 
   Size:       1619     511
 
EaeI y'GGCC_r

Cuts at:      0     851    2130 
   Size:        851    1279
 
EarI CTCTTCn'nnn_

Cuts at:      0     125     434     710     791     803    1508    1748    2130 
   Size:        125     309     276      81      12     705     240     382

  Fragments arranged by size: 

                 705     382     309     276     240     125      81      12
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     638     893    1091    1099    1412    1489    1684 
   Size:        236     402     255     198       8     313      77     195

Cuts at:   1684    1772    2130 
   Size:         88     358

  Fragments arranged by size: 

                 402     358     313     255     236     198     195      88
                  77       8
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1738    2130 
   Size:       1738     392
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1675    1820    2130 
   Size:       1675     145     310

  Fragments arranged by size: 

                1675     310     145
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1519    2130 
   Size:       1519     611
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     119     151    1048    1080    1354    1386 
   Size:         82      32       5      32     897      32     274      32

Cuts at:   1386    2130 
   Size:        744

  Fragments arranged by size: 

                 897     744     274      82      32      32      32      32
                   5
 
HindIII A'AGCT_T

Cuts at:      0    1613    2130 
   Size:       1613     517
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     912    1070    2130 
   Size:        912     158    1060

  Fragments arranged by size: 

                1060     912     158
 
MscI TGG'CCA

Cuts at:      0     853    2130 
   Size:        853    1277
 
MspA1I CmG'CkG

Cuts at:      0     244     535    2130 
   Size:        244     291    1595

  Fragments arranged by size: 

                1595     291     244
 
NcoI C'CATG_G

Cuts at:      0    1886    2130 
   Size:       1886     244
 
NruI TCG'CGA

Cuts at:      0      13    2130 
   Size:         13    2117
 
NsiI A_TGCA'T

Cuts at:      0    1578    2130 
   Size:       1578     552
 
PasI CC'CwG_GG

Cuts at:      0    1679    2130 
   Size:       1679     451
 
PpuMI rG'GwC_Cy

Cuts at:      0    1675    1820    2130 
   Size:       1675     145     310

  Fragments arranged by size: 

                1675     310     145
 
PvuII CAG'CTG

Cuts at:      0     244     535    2130 
   Size:        244     291    1595

  Fragments arranged by size: 

                1595     291     244
 
SapI GCTCTTCn'nnn_

Cuts at:      0     803    2130 
   Size:        803    1327
 
SfcI C'TryA_G

Cuts at:      0    1416    1482    2130 
   Size:       1416      66     648

  Fragments arranged by size: 

                1416     648      66
 
StuI AGG'CCT

Cuts at:      0    1733    2130 
   Size:       1733     397
 
StyI C'CwwG_G

Cuts at:      0     392    1877    1886    1922    2130 
   Size:        392    1485       9      36     208

  Fragments arranged by size: 

                1485     392     208      36       9
 
TatI w'GTAC_w

Cuts at:      0    1382    2130 
   Size:       1382     748
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1552    1884    2130 
   Size:       1552     332     246

  Fragments arranged by size: 

                1552     332     246
 
XmnI GAAnn'nnTTC

Cuts at:      0    1502    2130 
   Size:       1502     628
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     ApoI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI     BmrI     BpmI   Bpu10I    BsaXI    BseRI
    BseYI     BsmI    BspMI    BsrDI   BstAPI    BstBI   BstEII    BstXI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I  HindIII     MmeI
     MscI   MspA1I     NcoI     NruI     NsiI     PasI    PpuMI    PvuII
     SapI     SfcI     StuI     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI    BstYI  BstZ17I   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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