Restriction map for pF1KB5227
 (Linear) (Six-base) MAPSORT of: KIBB5227.seq  Check: 8287  from: 1  to: 1482



KIBB5227.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     765    1482 
   Size:        765     717
 
AccI GT'mk_AC

Cuts at:      0     133    1030    1482 
   Size:        133     897     452

  Fragments arranged by size: 

                 897     452     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46    1482 
   Size:         46    1436
 
AlwNI CAG_nnn'CTG

Cuts at:      0     142     772    1482 
   Size:        142     630     710

  Fragments arranged by size: 

                 710     630     142
 
ApoI r'AATT_y

Cuts at:      0     698    1482 
   Size:        698     784
 
AseI AT'TA_AT

Cuts at:      0     487    1482 
   Size:        487     995
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1174    1207    1482 
   Size:       1174      33     275

  Fragments arranged by size: 

                1174     275      33
 
BanI G'GyrC_C

Cuts at:      0    1404    1482 
   Size:       1404      78
 
BbvCI CC'TCA_GC

Cuts at:      0     401    1482 
   Size:        401    1081
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1351    1482 
   Size:       1351     131
 
BlpI GC'TnA_GC

Cuts at:      0     147    1482 
   Size:        147    1335
 
Bme1580I G_kGCm'C

Cuts at:      0    1179    1409    1482 
   Size:       1179     230      73

  Fragments arranged by size: 

                1179     230      73
 
BmgBI CAC'GTC

Cuts at:      0     691    1482 
   Size:        691     791
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     570     809    1482 
   Size:        570     239     673

  Fragments arranged by size: 

                 673     570     239
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     776    1482 
   Size:        455     321     706

  Fragments arranged by size: 

                 706     455     321
 
Bpu10I CC'TnA_GC

Cuts at:      0     401     923    1482 
   Size:        401     522     559

  Fragments arranged by size: 

                 559     522     401
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698     834    1482 
   Size:        698     136     648

  Fragments arranged by size: 

                 698     648     136
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1296    1443    1482 
   Size:       1296     147      39

  Fragments arranged by size: 

                1296     147      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     494    1418    1482 
   Size:        494     924      64

  Fragments arranged by size: 

                 924     494      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     743    1482 
   Size:        743     739
 
BsiHKAI G_wGCw'C

Cuts at:      0     459    1482 
   Size:        459    1023
 
BsmI GAATG_Cn'

Cuts at:      0     470     524     665    1482 
   Size:        470      54     141     817

  Fragments arranged by size: 

                 817     470     141      54
 
Bsp1286I G_dGCh'C

Cuts at:      0     459    1179    1409    1482 
   Size:        459     720     230      73

  Fragments arranged by size: 

                 720     459     230      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     156     765     786    1482 
   Size:        156     609      21     696

  Fragments arranged by size: 

                 696     609     156      21
 
BsrDI GCAATG_nn'

Cuts at:      0     156    1482 
   Size:        156    1326
 
BstYI r'GATC_y

Cuts at:      0      71    1482 
   Size:         71    1411
 
BstZ17I GTA'TAC

Cuts at:      0     134    1482 
   Size:        134    1348
 
BtgI C'CryG_G

Cuts at:      0      31    1482 
   Size:         31    1451
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     533     568    1482 
   Size:        533      35     914

  Fragments arranged by size: 

                 914     533      35
 
DraI TTT'AAA

Cuts at:      0     105    1482 
   Size:        105    1377
 
EaeI y'GGCC_r

Cuts at:      0    1210    1473    1482 
   Size:       1210     263       9

  Fragments arranged by size: 

                1210     263       9
 
EarI CTCTTCn'nnn_

Cuts at:      0    1160    1482 
   Size:       1160     322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     455     776    1482 
   Size:         46     409     321     706

  Fragments arranged by size: 

                 706     409     321      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     404     632    1211    1482 
   Size:        404     228     579     271

  Fragments arranged by size: 

                 579     404     271     228
 
EcoO109I rG'GnC_Cy

Cuts at:      0     139     940    1221    1482 
   Size:        139     801     281     261

  Fragments arranged by size: 

                 801     281     261     139
 
EcoRI G'AATT_C

Cuts at:      0     698    1482 
   Size:        698     784
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     875    1482 
   Size:        875     607
 
HaeII r_GCGC'y

Cuts at:      0     736    1482 
   Size:        736     746
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375    1141    1173    1482 
   Size:        343      32     766      32     309

  Fragments arranged by size: 

                 766     343     309      32      32
 
MfeI C'AATT_G

Cuts at:      0    1250    1482 
   Size:       1250     232
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     209    1224    1482 
   Size:        209    1015     258

  Fragments arranged by size: 

                1015     258     209
 
MslI CAynn'nnrTG

Cuts at:      0     601    1145    1482 
   Size:        601     544     337

  Fragments arranged by size: 

                 601     544     337
 
MspA1I CmG'CkG

Cuts at:      0      24     931    1078    1482 
   Size:         24     907     147     404

  Fragments arranged by size: 

                 907     404     147      24
 
NspI r_CATG'y

Cuts at:      0     165     543    1353    1482 
   Size:        165     378     810     129

  Fragments arranged by size: 

                 810     378     165     129
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     613     694    1243    1321    1482 
   Size:        613      81     549      78     161

  Fragments arranged by size: 

                 613     549     161      81      78
 
PfoI T'CCnGG_A

Cuts at:      0     693    1482 
   Size:        693     789
 
PpuMI rG'GwC_Cy

Cuts at:      0     139     940    1482 
   Size:        139     801     542

  Fragments arranged by size: 

                 801     542     139
 
PshAI GACnn'nnGTC

Cuts at:      0     838    1482 
   Size:        838     644
 
PstI C_TGCA'G

Cuts at:      0     366    1068    1482 
   Size:        366     702     414

  Fragments arranged by size: 

                 702     414     366
 
PvuII CAG'CTG

Cuts at:      0      24     931    1482 
   Size:         24     907     551

  Fragments arranged by size: 

                 907     551      24
 
ScaI AGT'ACT

Cuts at:      0    1062    1482 
   Size:       1062     420
 
SexAI A'CCwGG_T

Cuts at:      0     666    1482 
   Size:        666     816
 
SfcI C'TryA_G

Cuts at:      0     362     989    1031    1064    1482 
   Size:        362     627      42      33     418

  Fragments arranged by size: 

                 627     418     362      42      33
 
SmlI C'TyrA_G

Cuts at:      0     713     849    1482 
   Size:        713     136     633

  Fragments arranged by size: 

                 713     633     136
 
SpeI A'CTAG_T

Cuts at:      0    1045    1117    1482 
   Size:       1045      72     365

  Fragments arranged by size: 

                1045     365      72
 
SphI G_CATG'C

Cuts at:      0     165     543    1353    1482 
   Size:        165     378     810     129

  Fragments arranged by size: 

                 810     378     165     129
 
StyI C'CwwG_G

Cuts at:      0     936    1482 
   Size:        936     546
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     589     601    1482 
   Size:        589      12     881

  Fragments arranged by size: 

                 881     589      12
 
TatI w'GTAC_w

Cuts at:      0     853    1048    1060    1482 
   Size:        853     195      12     422

  Fragments arranged by size: 

                 853     422     195      12
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     943    1482 
   Size:        943     539
 
XmnI GAAnn'nnTTC

Cuts at:      0     251    1482 
   Size:        251    1231
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     ApoI     AseI     BaeI     BanI
    BbvCI    BciVI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrDI    BstYI  BstZ17I     BtgI    CspCI     DraI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     MfeI
     MmeI     MslI   MspA1I     NspI    PflMI     PfoI    PpuMI    PshAI
     PstI    PvuII     ScaI    SexAI     SfcI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII    BamHI    BanII     BbeI     BbsI     BcgI     BclI    BfrBI
     BglI    BglII     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
   Bsu36I    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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