Restriction map for pF1KB5205
 (Linear) (Six-base) MAPSORT of: KIBB5205.seq  Check: 4864  from: 1  to: 1629



KIBB5205.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     309    1629 
   Size:        309    1320
 
AccI GT'mk_AC

Cuts at:      0     239    1430    1629 
   Size:        239    1191     199

  Fragments arranged by size: 

                1191     239     199
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     976    1629 
   Size:        334     642     653

  Fragments arranged by size: 

                 653     642     334
 
AflIII A'CryG_T

Cuts at:      0     920    1629 
   Size:        920     709
 
AleI CACnn'nnGTG

Cuts at:      0     286    1629 
   Size:        286    1343
 
AlwNI CAG_nnn'CTG

Cuts at:      0      83    1296    1519    1629 
   Size:         83    1213     223     110

  Fragments arranged by size: 

                1213     223     110      83
 
AvaI C'yCGr_G

Cuts at:      0     613     730    1629 
   Size:        613     117     899

  Fragments arranged by size: 

                 899     613     117
 
BanI G'GyrC_C

Cuts at:      0     277    1129    1629 
   Size:        277     852     500

  Fragments arranged by size: 

                 852     500     277
 
BanII G_rGCy'C

Cuts at:      0     493     612     731    1398    1629 
   Size:        493     119     119     667     231

  Fragments arranged by size: 

                 667     493     231     119     119
 
BbeI G_GCGC'C

Cuts at:      0    1133    1629 
   Size:       1133     496
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     658    1629 
   Size:        658     971
 
BbvCI CC'TCA_GC

Cuts at:      0      98     263    1629 
   Size:         98     165    1366

  Fragments arranged by size: 

                1366     165      98
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1432    1466    1629 
   Size:       1432      34     163

  Fragments arranged by size: 

                1432     163      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1522    1629 
   Size:       1522     107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     894    1629 
   Size:        894     735
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1629 
   Size:        872     757
 
Bpu10I CC'TnA_GC

Cuts at:      0      98     263    1629 
   Size:         98     165    1366

  Fragments arranged by size: 

                1366     165      98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1254    1629 
   Size:       1254     375
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1042    1629 
   Size:       1042     587
 
BsaBI GATnn'nnATC

Cuts at:      0     586    1629 
   Size:        586    1043
 
BsaHI Gr'CG_yC

Cuts at:      0    1130    1629 
   Size:       1130     499
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     845     875    1629 
   Size:        845      30     754

  Fragments arranged by size: 

                 845     754      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     961    1162    1629 
   Size:        961     201     467

  Fragments arranged by size: 

                 961     467     201
 
BseYI C'CCAG_C

Cuts at:      0      59     386     506     667    1023    1629 
   Size:         59     327     120     161     356     606

  Fragments arranged by size: 

                 606     356     327     161     120      59
 
BsiHKAI G_wGCw'C

Cuts at:      0     493     731    1629 
   Size:        493     238     898

  Fragments arranged by size: 

                 898     493     238
 
BsmI GAATG_Cn'

Cuts at:      0     643    1008    1629 
   Size:        643     365     621

  Fragments arranged by size: 

                 643     621     365
 
Bsp1286I G_dGCh'C

Cuts at:      0     493     612     731    1398    1629 
   Size:        493     119     119     667     231

  Fragments arranged by size: 

                 667     493     231     119     119
 
BspHI T'CATG_A

Cuts at:      0    1178    1629 
   Size:       1178     451
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     309    1265    1629 
   Size:        309     956     364

  Fragments arranged by size: 

                 956     364     309
 
BsrBI CCG'CTC

Cuts at:      0     484    1158    1571    1629 
   Size:        484     674     413      58

  Fragments arranged by size: 

                 674     484     413      58
 
BsrDI GCAATG_nn'

Cuts at:      0     242    1448    1629 
   Size:        242    1206     181

  Fragments arranged by size: 

                1206     242     181
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     390     850    1239    1323    1629 
   Size:        390     460     389      84     306

  Fragments arranged by size: 

                 460     390     389     306      84
 
BstYI r'GATC_y

Cuts at:      0     839     951    1629 
   Size:        839     112     678

  Fragments arranged by size: 

                 839     678     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     944    1629 
   Size:        944     685
 
BtgI C'CryG_G

Cuts at:      0     126    1122    1608    1629 
   Size:        126     996     486      21

  Fragments arranged by size: 

                 996     486     126      21
 
BtsI GCAGTG_nn'

Cuts at:      0     551    1629 
   Size:        551    1078
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1158    1629 
   Size:       1158     471
 
EaeI y'GGCC_r

Cuts at:      0     893     933    1617    1629 
   Size:        893      40     684      12

  Fragments arranged by size: 

                 893     684      40      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     952    1040    1629 
   Size:        952      88     589

  Fragments arranged by size: 

                 952     589      88
 
EcoICRI GAG'CTC

Cuts at:      0     491     729    1629 
   Size:        491     238     900

  Fragments arranged by size: 

                 900     491     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     872     976    1629 
   Size:        334     538     104     653

  Fragments arranged by size: 

                 653     538     334     104
 
EcoO109I rG'GnC_Cy

Cuts at:      0     121    1629 
   Size:        121    1508
 
HaeII r_GCGC'y

Cuts at:      0    1133    1629 
   Size:       1133     496
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     971    1003    1418    1450    1629 
   Size:        971      32     415      32     179

  Fragments arranged by size: 

                 971     415     179      32      32
 
HincII GTy'rAC

Cuts at:      0     399    1629 
   Size:        399    1230
 
HindIII A'AGCT_T

Cuts at:      0     139     515    1466    1629 
   Size:        139     376     951     163

  Fragments arranged by size: 

                 951     376     163     139
 
HpaI GTT'AAC

Cuts at:      0     399    1629 
   Size:        399    1230
 
KasI G'GCGC_C

Cuts at:      0    1129    1629 
   Size:       1129     500
 
MfeI C'AATT_G

Cuts at:      0     794    1629 
   Size:        794     835
 
MscI TGG'CCA

Cuts at:      0     895    1629 
   Size:        895     734
 
MslI CAynn'nnrTG

Cuts at:      0     286    1237    1629 
   Size:        286     951     392

  Fragments arranged by size: 

                 951     392     286
 
MspA1I CmG'CkG

Cuts at:      0     390     627    1296    1344    1629 
   Size:        390     237     669      48     285

  Fragments arranged by size: 

                 669     390     285     237      48
 
NarI GG'CG_CC

Cuts at:      0    1130    1629 
   Size:       1130     499
 
NspI r_CATG'y

Cuts at:      0     924    1109    1405    1629 
   Size:        924     185     296     224

  Fragments arranged by size: 

                 924     296     224     185
 
PasI CC'CwG_GG

Cuts at:      0     117    1407    1629 
   Size:        117    1290     222

  Fragments arranged by size: 

                1290     222     117
 
PciI A'CATG_T

Cuts at:      0     920    1629 
   Size:        920     709
 
PfoI T'CCnGG_A

Cuts at:      0    1281    1629 
   Size:       1281     348
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1555    1587    1629 
   Size:       1555      32      42

  Fragments arranged by size: 

                1555      42      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     121    1629 
   Size:        121    1508
 
PspXI vC'TCGA_Gb

Cuts at:      0     613     730    1629 
   Size:        613     117     899

  Fragments arranged by size: 

                 899     613     117
 
PstI C_TGCA'G

Cuts at:      0     225     306     572    1629 
   Size:        225      81     266    1057

  Fragments arranged by size: 

                1057     266     225      81
 
PvuII CAG'CTG

Cuts at:      0     390     627    1296    1344    1629 
   Size:        390     237     669      48     285

  Fragments arranged by size: 

                 669     390     285     237      48
 
SacI G_AGCT'C

Cuts at:      0     493     731    1629 
   Size:        493     238     898

  Fragments arranged by size: 

                 898     493     238
 
SanDI GG'GwC_CC

Cuts at:      0     121    1629 
   Size:        121    1508
 
ScaI AGT'ACT

Cuts at:      0     904    1629 
   Size:        904     725
 
SfcI C'TryA_G

Cuts at:      0     221     302     568    1544    1629 
   Size:        221      81     266     976      85

  Fragments arranged by size: 

                 976     266     221      85      81
 
SfoI GGC'GCC

Cuts at:      0    1131    1629 
   Size:       1131     498
 
SmlI C'TyrA_G

Cuts at:      0     613     730    1269    1629 
   Size:        613     117     539     360

  Fragments arranged by size: 

                 613     539     360     117
 
SphI G_CATG'C

Cuts at:      0    1405    1629 
   Size:       1405     224
 
StuI AGG'CCT

Cuts at:      0    1321    1629 
   Size:       1321     308
 
StyI C'CwwG_G

Cuts at:      0      48    1094    1316    1322    1629 
   Size:         48    1046     222       6     307

  Fragments arranged by size: 

                1046     307     222      48       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     259     400     913    1629 
   Size:        259     141     513     716

  Fragments arranged by size: 

                 716     513     259     141
 
TatI w'GTAC_w

Cuts at:      0     902    1629 
   Size:        902     727
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       2      34    1629 
   Size:          2      32    1595

  Fragments arranged by size: 

                1595      32       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     888    1629 
   Size:        888     741
 
XhoI C'TCGA_G

Cuts at:      0     613     730    1629 
   Size:        613     117     899

  Fragments arranged by size: 

                 899     613     117
 
XmnI GAAnn'nnTTC

Cuts at:      0     517     655    1629 
   Size:        517     138     974

  Fragments arranged by size: 

                 974     517     138
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BglI     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BstXI
    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MscI     MslI   MspA1I     NarI     NspI     PasI     PciI
     PfoI     PpiI    PpuMI    PspXI     PstI    PvuII     SacI    SanDI
     ScaI     SfcI     SfoI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BsaAI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     EagI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
     KpnI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI    PshAI
     PsiI   PspOMI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI  Tth111I     XbaI     XmaI     ZraI
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