Restriction map for pF1KB5199
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BsaBI       BsaWI       BseAI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tsp509I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         931  GACGTC               GACGT^C
AlwNI        1114  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         601  GAGCAC               G[AT]GC[AT]^C
AspI          571  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1149  CCCGGG               C^[CT]CG[GA]G
BbsI          955  GTCTTC               GAAGAC(2/6)
BfaI          844  CTAG                 C^TAG
BglII         110  AGATCT               A^GATCT
BlpI          708  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      708  GCTCAGC              GC^T[ACGT]AGC
BpuAI         955  GTCTTC               GAAGAC(2/6)
BsaAI          91  TACGTG               [CT]AC^GT[GA]
BsiEI        1319  CGGCCG               CG[GA][CT]^CG
BsiHKAI       601  GAGCAC               G[AT]GC[AT]^C
BsmBI        1198  GAGACG               CGTCTC(1/5)
BsoBI        1149  CCCGGG               C^[CT]CG[GA]G
BspMI         725  ACCTGC               ACCTGC(4/8)
BssHII         44  GCGCGC               G^CGCGC
BstXI         872  CCACGCTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         708  GCTCAGC              GC^T[ACGT]AGC
DraII         180  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        403  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1319  CGGCCG               C^GGCCG
EarI         1084  CTCTTC               CTCTTC(1/4)
EclXI        1319  CGGCCG               C^GGCCG
Eco52I       1319  CGGCCG               C^GGCCG
EcoO109I      180  AGGCCCC              [GA]G^G[ACGT]CC[CT]
HaeII        1139  AGCGCC               [GA]GCGC^[CT]
HinfI         470  GAATC                G^A[ACGT]TC
Ksp632I      1084  CTCTTC               CTCTTC(1/4)
MaeI          844  CTAG                 C^TAG
NcoI          515  CCATGG               C^CATGG
PflFI         571  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         628  CAGCTG               CAG^CTG
SmaI         1149  CCCGGG               CCC^GGG
SphI          533  GCATGC               GCATG^C
TfiI          470  GAATC                G^A[AT]TC
Tth111I       571  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1149  CCCGGG               C^CCGGG
XmaI         1149  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          577  GTCTAC               GT^[AC][GT]AC
              934  GTCTAC              
AcyI          931  GACGTC               G[GA]^CG[CT]C
             1105  GGCGTC              
AvaII         498  GGACC                G^G[AT]CC
              540  GGACC               
BanI           61  GGCACC               G^G[CT][GA]CC
              825  GGTGCC              
BanII          96  GGGCTC               G[GA]GC[CT]^C
             1164  GGGCTC              
BsaHI         931  GACGTC               G[GA]^CG[CT]C
             1105  GGCGTC              
BseRI          37  CTCCTC               GAGGAG(10/8)
              894  CTCCTC              
BsgI          271  CTGCAC               GTGCAG(16/14)
              715  CTGCAC              
BsmFI         287  GGGAC                GGGAC(10/14)
             1147  GTCCC               
Bsp1407I      458  TGTACA               T^GTACA
              746  TGTACA              
BsrGI         458  TGTACA               T^GTACA
              746  TGTACA              
BstYI         110  AGATCT               [GA]^GATC[CT]
              944  GGATCT              
EcoT14I       515  CCATGG               C^C[AT][AT]GG
             1028  CCAAGG              
Hin1I         931  GACGTC               G[GA]^CG[CT]C
             1105  GGCGTC              
KspI           57  CCGCGG               CCGC^GG
              738  CCGCGG              
MflI          110  AGATCT               [GA]^GATC[CT]
              944  GGATCT              
NspI          533  GCATGC               [GA]CATG^[CT]
              764  ACATGC              
PflMI         609  CCACGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              788  CCATGTCCTGG         
PstI          436  CTGCAG               CTGCA^G
              625  CTGCAG              
SacII          57  CCGCGG               CCGC^GG
              738  CCGCGG              
SspBI         458  TGTACA               T^GTACA
              746  TGTACA              
StyI          515  CCATGG               C^C[AT][AT]GG
             1028  CCAAGG              
TaqI          224  TCGA                 T^CGA
             1265  TCGA                
Tsp45I        409  GTGAC                ^GT[GC]AC
             1108  GTCAC               
TthHB8I       224  TCGA                 T^CGA
             1265  TCGA                
Van91I        609  CCACGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              788  CCATGTCCTGG         
XhoII         110  AGATCT               [GA]^GATC[CT]
              944  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
BalI        BbvI        BcnI        BglI        BmyI        
BpmI        BsaI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI       XcmI        
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