Restriction map for pF1KB5199
 (Linear) (Six-base) MAPSORT of: KIBB5199.seq  Check: 7985  from: 1  to: 1356



KIBB5199.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     734    1356 
   Size:        734     622
 
AatII G_ACGT'C

Cuts at:      0     935    1356 
   Size:        935     421
 
AccI GT'mk_AC

Cuts at:      0     578     935    1356 
   Size:        578     357     421

  Fragments arranged by size: 

                 578     421     357
 
AleI CACnn'nnGTG

Cuts at:      0     856    1356 
   Size:        856     500
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     670     702    1356 
   Size:        670      32     654

  Fragments arranged by size: 

                 670     654      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1119    1356 
   Size:       1119     237
 
AvaI C'yCGr_G

Cuts at:      0    1149    1356 
   Size:       1149     207
 
BanI G'GyrC_C

Cuts at:      0      61     825    1356 
   Size:         61     764     531

  Fragments arranged by size: 

                 764     531      61
 
BanII G_rGCy'C

Cuts at:      0     100    1168    1356 
   Size:        100    1068     188

  Fragments arranged by size: 

                1068     188     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     948    1356 
   Size:        948     408
 
BglI GCCn_nnn'nGGC

Cuts at:      0      15     391     922    1238    1356 
   Size:         15     376     531     316     118

  Fragments arranged by size: 

                 531     376     316     118      15
 
BglII A'GATC_T

Cuts at:      0     110    1356 
   Size:        110    1246
 
BlpI GC'TnA_GC

Cuts at:      0     709    1356 
   Size:        709     647
 
Bme1580I G_kGCm'C

Cuts at:      0     830     891    1251    1356 
   Size:        830      61     360     105

  Fragments arranged by size: 

                 830     360     105      61
 
BmgBI CAC'GTC

Cuts at:      0     408    1065    1356 
   Size:        408     657     291

  Fragments arranged by size: 

                 657     408     291
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1347    1356 
   Size:       1347       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     229     565    1026    1356 
   Size:        127     102     336     461     330

  Fragments arranged by size: 

                 461     336     330     127     102
 
Bpu10I CC'TnA_GC

Cuts at:      0    1224    1255    1356 
   Size:       1224      31     101

  Fragments arranged by size: 

                1224     101      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     313     804    1044    1356 
   Size:        313     491     240     312

  Fragments arranged by size: 

                 491     313     312     240
 
BsaAI yAC'GTr

Cuts at:      0      93    1356 
   Size:         93    1263
 
BsaHI Gr'CG_yC

Cuts at:      0     932    1106    1356 
   Size:        932     174     250

  Fragments arranged by size: 

                 932     250     174
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      82     112     754     784    1356 
   Size:         82      30     642      30     572

  Fragments arranged by size: 

                 642     572      82      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28     885    1356 
   Size:         28     857     471

  Fragments arranged by size: 

                 857     471      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     700    1356 
   Size:        256     444     656

  Fragments arranged by size: 

                 656     444     256
 
BsiEI CG_ry'CG

Cuts at:      0    1322    1356 
   Size:       1322      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     605    1356 
   Size:        605     751
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1192    1356 
   Size:       1192     164
 
Bsp1286I G_dGCh'C

Cuts at:      0     100     605     830     891    1168    1251    1356 
   Size:        100     505     225      61     277      83     105

  Fragments arranged by size: 

                 505     277     225     105     100      83      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     734    1356 
   Size:        734     622
 
BsrBI CCG'CTC

Cuts at:      0      30     893    1223    1356 
   Size:         30     863     330     133

  Fragments arranged by size: 

                 863     330     133      30
 
BsrFI r'CCGG_y

Cuts at:      0     145    1102    1133    1356 
   Size:        145     957      31     223

  Fragments arranged by size: 

                 957     223     145      31
 
BsrGI T'GTAC_A

Cuts at:      0     458     746    1356 
   Size:        458     288     610

  Fragments arranged by size: 

                 610     458     288
 
BssHII G'CGCG_C

Cuts at:      0      44    1356 
   Size:         44    1312
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     879    1356 
   Size:        879     477
 
BstYI r'GATC_y

Cuts at:      0     110     944    1356 
   Size:        110     834     412

  Fragments arranged by size: 

                 834     412     110
 
BtgI C'CryG_G

Cuts at:      0      57     515     609     665     703     738     854     904 
   Size:         57     458      94      56      38      35     116      50

Cuts at:    904    1356 
   Size:        452

  Fragments arranged by size: 

                 458     452     116      94      57      56      50      38
                  35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      85    1356 
   Size:         85    1271
 
DraIII CAC_nnn'GTG

Cuts at:      0     408    1356 
   Size:        408     948
 
EaeI y'GGCC_r

Cuts at:      0     215     503     693    1121    1319    1356 
   Size:        215     288     190     428     198      37

  Fragments arranged by size: 

                 428     288     215     198     190      37
 
EagI C'GGCC_G

Cuts at:      0    1319    1356 
   Size:       1319      37
 
EarI CTCTTCn'nnn_

Cuts at:      0    1090    1356 
   Size:       1090     266
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     229     565    1026    1356 
   Size:        127     102     336     461     330

  Fragments arranged by size: 

                 461     336     330     127     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     181    1356 
   Size:        181    1175
 
HaeII r_GCGC'y

Cuts at:      0    1143    1356 
   Size:       1143     213
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249    1356 
   Size:        217      32    1107

  Fragments arranged by size: 

                1107     217      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     125     569    1356 
   Size:        125     444     787

  Fragments arranged by size: 

                 787     444     125
 
MscI TGG'CCA

Cuts at:      0     217     505     695    1123    1356 
   Size:        217     288     190     428     233

  Fragments arranged by size: 

                 428     288     233     217     190
 
MslI CAynn'nnrTG

Cuts at:      0     262     856     877    1356 
   Size:        262     594      21     479

  Fragments arranged by size: 

                 594     479     262      21
 
MspA1I CmG'CkG

Cuts at:      0      59     630     691     740    1356 
   Size:         59     571      61      49     616

  Fragments arranged by size: 

                 616     571      61      59      49
 
NaeI GCC'GGC

Cuts at:      0     147    1104    1135    1356 
   Size:        147     957      31     221

  Fragments arranged by size: 

                 957     221     147      31
 
NcoI C'CATG_G

Cuts at:      0     515    1356 
   Size:        515     841
 
NgoMIV G'CCGG_C

Cuts at:      0     145    1102    1133    1356 
   Size:        145     957      31     223

  Fragments arranged by size: 

                 957     223     145      31
 
NspI r_CATG'y

Cuts at:      0     537     768    1356 
   Size:        537     231     588

  Fragments arranged by size: 

                 588     537     231
 
PasI CC'CwG_GG

Cuts at:      0     324    1356 
   Size:        324    1032
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     615     794    1356 
   Size:        615     179     562

  Fragments arranged by size: 

                 615     562     179
 
PstI C_TGCA'G

Cuts at:      0     440     629    1356 
   Size:        440     189     727

  Fragments arranged by size: 

                 727     440     189
 
PvuII CAG'CTG

Cuts at:      0     630    1356 
   Size:        630     726
 
SacII CC_GC'GG

Cuts at:      0      60     741    1356 
   Size:         60     681     615

  Fragments arranged by size: 

                 681     615      60
 
SfcI C'TryA_G

Cuts at:      0     436     625    1008    1170    1356 
   Size:        436     189     383     162     186

  Fragments arranged by size: 

                 436     383     189     186     162
 
SmaI CCC'GGG

Cuts at:      0    1151    1356 
   Size:       1151     205
 
SphI G_CATG'C

Cuts at:      0     537    1356 
   Size:        537     819
 
StyI C'CwwG_G

Cuts at:      0     515    1028    1356 
   Size:        515     513     328

  Fragments arranged by size: 

                 515     513     328
 
TatI w'GTAC_w

Cuts at:      0     251     458     746    1356 
   Size:        251     207     288     610

  Fragments arranged by size: 

                 610     288     251     207
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     340     372    1356 
   Size:        340      32     984

  Fragments arranged by size: 

                 984     340      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     574    1356 
   Size:        574     782
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     321     513     924    1356 
   Size:        321     192     411     432

  Fragments arranged by size: 

                 432     411     321     192
 
XmaI C'CCGG_G

Cuts at:      0    1149    1356 
   Size:       1149     207
 
ZraI GAC'GTC

Cuts at:      0     933    1356 
   Size:        933     423
 

 Enzymes that do cut:

     AarI    AatII     AccI     AleI     AloI    AlwNI     AvaI     BanI
    BanII     BbsI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BstXI    BstYI     BtgI    BtgZI   DraIII     EaeI     EagI
     EarI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PstI
    PvuII    SacII     SfcI     SmaI     SphI     StyI     TatI     TstI
  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BmtI     BplI    BpuEI    BsaBI    BsaWI    BseYI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     XbaI     XhoI     XmnI
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