Restriction map for pF1KB5164
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BbeI        BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BsaBI       BsaHI       
BsaI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MseI        MslI        MunI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tru9I       Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII          95  CGCG                 CG^CG
AccIII        369  TCCGGA               T^CCGGA
AcsI          234  GAATTT               [GA]^AATT[CT]
AocI         1113  CCTCAGG              CC^T[ACGT]AGG
ApoI          234  GAATTT               [GA]^AATT[CT]
Asp700I       409  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          721  TGGCCA               TGG^CCA
BamHI          20  GGATCC               G^GATCC
BbrPI        1107  CACGTG               CAC^GTG
BbvCI         502  CCTCAGC              CCTCAGC(-5/-2)
BglII         981  AGATCT               A^GATCT
BlpI          792  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      792  GCTGAGC              GC^T[ACGT]AGC
BseAI         369  TCCGGA               T^CCGGA
BsgI         1177  CTGCAC               GTGCAG(16/14)
BsmAI         701  GAGAC                GTCTC(1/5)
BspEI         369  TCCGGA               T^CCGGA
BsrBI         240  CCGCTC               CCGCTC(-3/-3)
BssSI        1084  CTCGTG               CACGAG(-5/-1)
BstUI          95  CGCG                 CG^CG
BstXI        1263  CCAGGGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1113  CCTCAGG              CC^T[ACGT]AGG
CelII         792  GCTGAGC              GC^T[ACGT]AGC
CfoI           96  GCGC                 GCG^C
EaeI          721  TGGCCA               [CT]^GGCC[GA]
Eco81I       1113  CCTCAGG              CC^T[ACGT]AGG
HhaI           96  GCGC                 GCG^C
HinP1I         96  GCGC                 G^CGC
MluNI         721  TGGCCA               TGG^CCA
MroI          369  TCCGGA               T^CCGGA
MscI          721  TGGCCA               TGG^CCA
MvnI           95  CGCG                 CG^CG
NdeI          188  CATATG               CA^TATG
PleI         1048  GAGTC                GAGTC(4/5)
PmaCI        1107  CACGTG               CAC^GTG
PmlI         1107  CACGTG               CAC^GTG
PstI         1234  CTGCAG               CTGCA^G
PvuII          43  CAGCTG               CAG^CTG
SbfI         1233  CCTGCAGG             CCTGCA^GG
ScaI          391  AGTACT               AGT^ACT
Sse8387I     1233  CCTGCAGG             CCTGCA^GG
TaqI          611  TCGA                 T^CGA
TfiI          155  GAATC                G^A[AT]TC
Tsp509I       235  AATT                 ^AATT
TthHB8I       611  TCGA                 T^CGA
XmnI          409  GAAGACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI          392  GTAC                 GT^AC
             1221  GTAC                
AvaI          196  CCCGGG               C^[CT]CG[GA]G
             1136  CCCGGG              
BanI          588  GGTGCC               G^G[CT][GA]CC
             1147  GGTGCC              
BbsI          409  GAAGAC               GAAGAC(2/6)
              643  GTCTTC              
BglI          665  GCCCGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              714  GCCTGGATGGC         
BpmI           61  CTGGAG               CTGGAG(16/14)
             1159  CTGGAG              
BpuAI         409  GAAGAC               GAAGAC(2/6)
              643  GTCTTC              
BsaAI         778  TACGTG               [CT]AC^GT[GA]
             1107  CACGTG              
BsaWI         284  ACCGGA               [AT]^CCGG[AT]
              369  TCCGGA              
BseRI        1152  CTCCTC               GAGGAG(10/8)
              889  GAGGAG              
BsoBI         196  CCCGGG               C^[CT]CG[GA]G
             1136  CCCGGG              
DsaI           16  CCACGG               C^C[GA][CT]GG
             1069  CCGTGG              
EarI          228  GAAGAG               CTCTTC(1/4)
              598  GAAGAG              
EcoNI         123  CCTACGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              224  CCTGGAAGAGG         
FauI          825  GCGGG                CCCGC(4/6)
              908  GCGGG               
HgaI          708  GACGC                GACGC(5/10)
             1010  GACGC               
HinfI         155  GAATC                G^A[ACGT]TC
             1048  GAGTC               
Ksp632I       228  GAAGAG               CTCTTC(1/4)
              598  GAAGAG              
MaeII         779  ACGT                 A^CGT
             1108  ACGT                
NspI          746  GCATGT               [GA]CATG^[CT]
              786  GCATGT              
PflMI         736  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              849  CCAGCGGCTGG         
RsaI          392  GTAC                 GT^AC
             1221  GTAC                
SfaNI         133  GATGC                GCATC(5/9)
              104  GCATC               
SfcI          482  CTGTAG               C^T[GA][CT]AG
             1234  CTGCAG              
SmaI          196  CCCGGG               CCC^GGG
             1136  CCCGGG              
StuI          164  AGGCCT               AGG^CCT
             1226  AGGCCT              
Tsp45I        616  GTGAC                ^GT[GC]AC
              727  GTCAC               
Van91I        736  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              849  CCAGCGGCTGG         
XmaCI         196  CCCGGG               C^CCGGG
             1136  CCCGGG              
XmaI          196  CCCGGG               C^CCGGG
             1136  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       ApaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
Sau3AI      Sau96I      ScrFI       StyI        TseI        
TspRI       XhoII       
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