Restriction map for pF1KB5164
 (Linear) (Six-base) MAPSORT of: KIBB5164.seq  Check: 7542  from: 1  to: 1281



KIBB5164.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     595    1281 
   Size:        595     686
 
AlwNI CAG_nnn'CTG

Cuts at:      0     510     742     855    1281 
   Size:        510     232     113     426

  Fragments arranged by size: 

                 510     426     232     113
 
ApaI G_GGCC'C

Cuts at:      0     560     849     971    1270    1281 
   Size:        560     289     122     299      11

  Fragments arranged by size: 

                 560     299     289     122      11
 
ApoI r'AATT_y

Cuts at:      0     234    1281 
   Size:        234    1047
 
AvaI C'yCGr_G

Cuts at:      0     196    1136    1281 
   Size:        196     940     145

  Fragments arranged by size: 

                 940     196     145
 
BamHI G'GATC_C

Cuts at:      0      20    1281 
   Size:         20    1261
 
BanI G'GyrC_C

Cuts at:      0     588    1147    1281 
   Size:        588     559     134

  Fragments arranged by size: 

                 588     559     134
 
BanII G_rGCy'C

Cuts at:      0     497     520     560     849     971    1270    1281 
   Size:        497      23      40     289     122     299      11

  Fragments arranged by size: 

                 497     299     289     122      40      23      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     416     636    1281 
   Size:        416     220     645

  Fragments arranged by size: 

                 645     416     220
 
BbvCI CC'TCA_GC

Cuts at:      0     503    1281 
   Size:        503     778
 
BglI GCCn_nnn'nGGC

Cuts at:      0     671     720    1281 
   Size:        671      49     561

  Fragments arranged by size: 

                 671     561      49
 
BglII A'GATC_T

Cuts at:      0     981    1281 
   Size:        981     300
 
BlpI GC'TnA_GC

Cuts at:      0     793    1281 
   Size:        793     488
 
Bme1580I G_kGCm'C

Cuts at:      0     560     849     863     971    1106    1270    1281 
   Size:        560     289      14     108     135     164      11

  Fragments arranged by size: 

                 560     289     164     135     108      14      11
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     439     885    1281 
   Size:        439     446     396

  Fragments arranged by size: 

                 446     439     396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1180    1281 
   Size:         82    1098     101

  Fragments arranged by size: 

                1098     101      82
 
Bpu10I CC'TnA_GC

Cuts at:      0     491     503    1281 
   Size:        491      12     778

  Fragments arranged by size: 

                 778     491      12
 
BsaAI yAC'GTr

Cuts at:      0     780    1109    1281 
   Size:        780     329     172

  Fragments arranged by size: 

                 780     329     172
 
BsaWI w'CCGG_w

Cuts at:      0     284     369    1281 
   Size:        284      85     912

  Fragments arranged by size: 

                 912     284      85
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1186    1216    1281 
   Size:       1186      30      65

  Fragments arranged by size: 

                1186      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     904    1143    1281 
   Size:        904     239     138

  Fragments arranged by size: 

                 904     239     138
 
BseYI C'CCAG_C

Cuts at:      0     353     520     848     855    1281 
   Size:        353     167     328       7     426

  Fragments arranged by size: 

                 426     353     328     167       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1162    1281 
   Size:       1162     119
 
Bsp1286I G_dGCh'C

Cuts at:      0     497     520     560     849     863     971    1106    1270 
   Size:        497      23      40     289      14     108     135     164

Cuts at:   1270    1281 
   Size:         11

  Fragments arranged by size: 

                 497     289     164     135     108      40      23      14
                  11
 
BspEI T'CCGG_A

Cuts at:      0     369    1281 
   Size:        369     912
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     224    1184    1241    1281 
   Size:        224     960      57      40

  Fragments arranged by size: 

                 960     224      57      40
 
BsrBI CCG'CTC

Cuts at:      0     242    1281 
   Size:        242    1039
 
BssSI C'ACGA_G

Cuts at:      0    1084    1281 
   Size:       1084     197
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1270    1281 
   Size:       1270      11
 
BstYI r'GATC_y

Cuts at:      0      20     459     981    1281 
   Size:         20     439     522     300

  Fragments arranged by size: 

                 522     439     300      20
 
Bsu36I CC'TnA_GG

Cuts at:      0    1114    1281 
   Size:       1114     167
 
BtgI C'CryG_G

Cuts at:      0      16    1069    1281 
   Size:         16    1053     212

  Fragments arranged by size: 

                1053     212      16
 
BtsI GCAGTG_nn'

Cuts at:      0     730     841     861     887    1281 
   Size:        730     111      20      26     394

  Fragments arranged by size: 

                 730     394     111      26      20
 
EaeI y'GGCC_r

Cuts at:      0     721    1281 
   Size:        721     560
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     593    1281 
   Size:        223     370     688

  Fragments arranged by size: 

                 688     370     223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     595    1180    1281 
   Size:         82     513     585     101

  Fragments arranged by size: 

                 585     513     101      82
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     127     228    1281 
   Size:        127     101    1053

  Fragments arranged by size: 

                1053     127     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0     294     466     556     557     672     930     967     968 
   Size:        294     172      90       1     115     258      37       1

Cuts at:    968    1118    1266    1267    1281 
   Size:        150     148       1      14

  Fragments arranged by size: 

                 294     258     172     150     148     115      90      37
                  14       1       1       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     710     742     803     835    1281 
   Size:        710      32      61      32     446

  Fragments arranged by size: 

                 710     446      61      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1211    1238    1281 
   Size:       1211      27      43

  Fragments arranged by size: 

                1211      43      27
 
MscI TGG'CCA

Cuts at:      0     723    1281 
   Size:        723     558
 
MspA1I CmG'CkG

Cuts at:      0      45     852     908    1281 
   Size:         45     807      56     373

  Fragments arranged by size: 

                 807     373      56      45
 
NdeI CA'TA_TG

Cuts at:      0     189    1281 
   Size:        189    1092
 
NspI r_CATG'y

Cuts at:      0     750     790    1281 
   Size:        750      40     491

  Fragments arranged by size: 

                 750     491      40
 
PasI CC'CwG_GG

Cuts at:      0    1270    1281 
   Size:       1270      11
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     742     855    1281 
   Size:        742     113     426

  Fragments arranged by size: 

                 742     426     113
 
PfoI T'CCnGG_A

Cuts at:      0     462    1281 
   Size:        462     819
 
PmlI CAC'GTG

Cuts at:      0    1109    1281 
   Size:       1109     172
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     592     624    1281 
   Size:        592      32     657

  Fragments arranged by size: 

                 657     592      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     294     466     930    1281 
   Size:        294     172     464     351

  Fragments arranged by size: 

                 464     351     294     172
 
PspOMI G'GGCC_C

Cuts at:      0     556     845     967    1266    1281 
   Size:        556     289     122     299      15

  Fragments arranged by size: 

                 556     299     289     122      15
 
PstI C_TGCA'G

Cuts at:      0    1238    1281 
   Size:       1238      43
 
PvuII CAG'CTG

Cuts at:      0      45    1281 
   Size:         45    1236
 
SanDI GG'GwC_CC

Cuts at:      0     466    1281 
   Size:        466     815
 
SbfI CC_TGCA'GG

Cuts at:      0    1238    1281 
   Size:       1238      43
 
ScaI AGT'ACT

Cuts at:      0     393    1281 
   Size:        393     888
 
SfcI C'TryA_G

Cuts at:      0     482    1234    1281 
   Size:        482     752      47

  Fragments arranged by size: 

                 752     482      47
 
SmaI CCC'GGG

Cuts at:      0     198    1138    1281 
   Size:        198     940     143

  Fragments arranged by size: 

                 940     198     143
 
StuI AGG'CCT

Cuts at:      0     166    1228    1281 
   Size:        166    1062      53

  Fragments arranged by size: 

                1062     166      53
 
StyI C'CwwG_G

Cuts at:      0     109     935    1121    1281 
   Size:        109     826     186     160

  Fragments arranged by size: 

                 826     186     160     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      66     793    1211    1281 
   Size:         66     727     418      70

  Fragments arranged by size: 

                 727     418      70      66
 
TatI w'GTAC_w

Cuts at:      0     391    1220    1281 
   Size:        391     829      61

  Fragments arranged by size: 

                 829     391      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1054    1086    1186    1218    1281 
   Size:       1054      32     100      32      63

  Fragments arranged by size: 

                1054     100      63      32      32
 
XmaI C'CCGG_G

Cuts at:      0     196    1136    1281 
   Size:        196     940     145

  Fragments arranged by size: 

                 940     196     145
 
XmnI GAAnn'nnTTC

Cuts at:      0     413    1281 
   Size:        413     868
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI Bsp1286I
    BspEI    BspMI    BsrBI    BssSI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    Hin4I     MmeI
     MscI   MspA1I     NdeI     NspI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI     PstI    PvuII    SanDI     SbfI     ScaI
     SfcI     SmaI     StuI     StyI    TaqII     TatI     TstI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI
     XcmI     XhoI     ZraI
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