Restriction map for pF1KB5154
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspI        AviII       BamHI       BbvCI       
BcgI        BclI        BfrI        BglI        BsiWI       
BsmI        BspDI       BspLU11I    BspMI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      ClaI        CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MfeI        MluI        MunI        MvnI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SphI        Sse8387I    StuI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccIII        476  TCCGGA               T^CCGGA
AseI         1468  ATTAAT               AT^TAAT
AsnI         1468  ATTAAT               AT^TAAT
AvaI         1063  CCCGAG               C^[CT]CG[GA]G
AvrII        1329  CCTAGG               C^CTAGG
BbeI         1798  GGCGCC               GGCGC^C
BbrPI        1837  CACGTG               CAC^GTG
BglII        1178  AGATCT               A^GATCT
BlnI         1329  CCTAGG               C^CTAGG
BsaAI        1837  CACGTG               [CT]AC^GT[GA]
BsaWI         476  TCCGGA               [AT]^CCGG[AT]
BseAI         476  TCCGGA               T^CCGGA
BsgI         1713  CTGCAC               GTGCAG(16/14)
BsiEI          59  CGGTCG               CG[GA][CT]^CG
BsmBI        1448  GAGACG               CGTCTC(1/5)
BsoBI        1063  CCCGAG               C^[CT]CG[GA]G
BspEI         476  TCCGGA               T^CCGGA
BspHI         416  TCATGA               T^CATGA
BstAPI        440  GCAACGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI          846  TTTAAA               TTT^AAA
EcoNI          46  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI         1798  GGCGCC               G^GCGCC
MroI          476  TCCGGA               T^CCGGA
MslI          451  CATCACAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1798  GGCGCC               GG^CGCC
NcoI         1873  CCATGG               C^CATGG
PleI         1605  GACTC                GAGTC(4/5)
PmaCI        1837  CACGTG               CAC^GTG
PmlI         1837  CACGTG               CAC^GTG
PshBI        1468  ATTAAT               AT^TAAT
RcaI          416  TCATGA               T^CATGA
SacI         1163  GAGCTC               GAGCT^C
SapI         1590  GAAGAGC              GCTCTTC(1/4)
SfaNI        1287  GATGC                GCATC(5/9)
SfoI         1798  GGCGCC               GGC^GCC
SpeI          835  ACTAGT               A^CTAGT
SspI         1623  AATATT               AAT^ATT
SwaI          845  ATTTAAAT             ATTT^AAAT
TfiI          229  GAATC                G^A[AT]TC
XcmI         1404  CCACCAGTCCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII        1450  GACGTC               GACGT^C
             1825  GACGTC              
AccI          407  GTCTAC               GT^[AC][GT]AC
              831  GTCTAC              
AlwNI          17  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1119  CAGAGCCTG           
AspHI        1163  GAGCTC               G[AT]GC[AT]^C
             1810  GAGCAC              
BbsI          561  GAAGAC               GAAGAC(2/6)
             1480  GAAGAC              
BlpI          964  GCTCAGC              GC^T[ACGT]AGC
             1036  GCTGAGC             
BpmI          128  CTGGAG               CTGGAG(16/14)
             1106  CTGGAG              
Bpu1102I      964  GCTCAGC              GC^T[ACGT]AGC
             1036  GCTGAGC             
BpuAI         561  GAAGAC               GAAGAC(2/6)
             1480  GAAGAC              
BsaBI         550  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1015  GATGGCTATC          
BsaI          636  GAGACC               GGTCTC(1/5)
             1109  GAGACC              
BseRI         122  CTCCTC               GAGGAG(10/8)
              887  GAGGAG              
BsiHKAI      1163  GAGCTC               G[AT]GC[AT]^C
             1810  GAGCAC              
Bsp1407I      358  TGTACA               T^GTACA
             1703  TGTACA              
BsrBI         119  CCGCTC               CCGCTC(-3/-3)
               56  GAGCGG              
BsrFI          99  GCCGGT               [GA]^CCGG[CT]
             1190  ACCGGC              
BsrGI         358  TGTACA               T^GTACA
             1703  TGTACA              
BstXI         450  CCATCACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1407  CCAGTCCATTGG        
CelII         964  GCTCAGC              GC^T[ACGT]AGC
             1036  GCTGAGC             
Cfr10I         99  GCCGGT               [GA]^CCGG[CT]
             1190  ACCGGC              
DrdI          528  GACATGGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1491  GACAGCCAAGTC        
EcoT14I      1329  CCTAGG               C^C[AT][AT]GG
             1873  CCATGG              
HaeII         968  AGCGCC               [GA]GCGC^[CT]
             1798  GGCGCC              
HinfI         229  GAATC                G^A[ACGT]TC
             1605  GACTC               
MamI          550  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1015  GATGGCTATC          
PpuMI          18  AGGACCT              [GA]G^G[AT]CC[CT]
              776  AGGACCT             
SfcI          426  CTACAG               C^T[GA][CT]AG
             1116  CTACAG              
SspBI         358  TGTACA               T^GTACA
             1703  TGTACA              
StyI         1329  CCTAGG               C^C[AT][AT]GG
             1873  CCATGG              
TaqI          203  TCGA                 T^CGA
             1161  TCGA                
Tsp45I        947  GTGAC                ^GT[GC]AC
             1784  GTGAC               
TthHB8I       203  TCGA                 T^CGA
             1161  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AvaII       BalI        BanI        
BanII       BbvI        BciVI       BcnI        BfaI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       Tru9I       
TseI        Tsp509I     TspRI       XhoII       
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