Restriction map for pF1KB5122
 (Linear) (Six-base) MAPSORT of: KIBB5122.seq  Check: 7820  from: 1  to: 1542



KIBB5122.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:27  ..

AccI GT'mk_AC

Cuts at:      0     168     225     642     857    1286    1542 
   Size:        168      57     417     215     429     256

  Fragments arranged by size: 

                 429     417     256     215     168      57
 
Acc65I G'GTAC_C

Cuts at:      0     208    1542 
   Size:        208    1334
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530    1542 
   Size:        530    1012
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1436    1542 
   Size:       1436     106
 
AlwNI CAG_nnn'CTG

Cuts at:      0     328     331     562    1542 
   Size:        328       3     231     980

  Fragments arranged by size: 

                 980     328     231       3
 
ApoI r'AATT_y

Cuts at:      0     834    1178    1542 
   Size:        834     344     364

  Fragments arranged by size: 

                 834     364     344
 
AseI AT'TA_AT

Cuts at:      0     197    1542 
   Size:        197    1345
 
BanI G'GyrC_C

Cuts at:      0     121     208    1542 
   Size:        121      87    1334

  Fragments arranged by size: 

                1334     121      87
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1280    1314    1542 
   Size:       1280      34     228

  Fragments arranged by size: 

                1280     228      34
 
BfrBI ATG'CAT

Cuts at:      0    1301    1542 
   Size:       1301     241
 
Bme1580I G_kGCm'C

Cuts at:      0     126    1542 
   Size:        126    1416
 
BmtI G_CTAG'C

Cuts at:      0     779    1542 
   Size:        779     763
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584     872    1542 
   Size:        584     288     670

  Fragments arranged by size: 

                 670     584     288
 
BsaBI GATnn'nnATC

Cuts at:      0     151    1300    1542 
   Size:        151    1149     242

  Fragments arranged by size: 

                1149     242     151
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1475    1505    1542 
   Size:       1475      30      37

  Fragments arranged by size: 

                1475      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415    1542 
   Size:        415    1127
 
BsiHKAI G_wGCw'C

Cuts at:      0     414    1425    1542 
   Size:        414    1011     117

  Fragments arranged by size: 

                1011     414     117
 
BsmI GAATG_Cn'

Cuts at:      0     989    1479    1542 
   Size:        989     490      63

  Fragments arranged by size: 

                 989     490      63
 
Bsp1286I G_dGCh'C

Cuts at:      0     126     414    1425    1542 
   Size:        126     288    1011     117

  Fragments arranged by size: 

                1011     288     126     117
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1352    1542 
   Size:       1352     190
 
BsrGI T'GTAC_A

Cuts at:      0    1334    1395    1542 
   Size:       1334      61     147

  Fragments arranged by size: 

                1334     147      61
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     328     331    1542 
   Size:        328       3    1211

  Fragments arranged by size: 

                1211     328       3
 
BstEII G'GTnAC_C

Cuts at:      0     766    1542 
   Size:        766     776
 
BstYI r'GATC_y

Cuts at:      0     364    1542 
   Size:        364    1178
 
BstZ17I GTA'TAC

Cuts at:      0     643    1542 
   Size:        643     899
 
Bsu36I CC'TnA_GG

Cuts at:      0     783    1542 
   Size:        783     759
 
BtsI GCAGTG_nn'

Cuts at:      0      18    1542 
   Size:         18    1524
 
DraI TTT'AAA

Cuts at:      0    1118    1542 
   Size:       1118     424
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1147    1542 
   Size:       1147     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     584     872    1542 
   Size:        530      54     288     670

  Fragments arranged by size: 

                 670     530     288      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     799    1071    1542 
   Size:        799     272     471

  Fragments arranged by size: 

                 799     471     272
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     730    1397    1542 
   Size:        730     667     145

  Fragments arranged by size: 

                 730     667     145
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     670     702    1206    1238    1407    1439 
   Size:        226      32     412      32     504      32     169      32

Cuts at:   1439    1542 
   Size:        103

  Fragments arranged by size: 

                 504     412     226     169     103      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0      24     226    1542 
   Size:         24     202    1316

  Fragments arranged by size: 

                1316     202      24
 
HindIII A'AGCT_T

Cuts at:      0     290     304    1542 
   Size:        290      14    1238

  Fragments arranged by size: 

                1238     290      14
 
KpnI G_GTAC'C

Cuts at:      0     212    1542 
   Size:        212    1330
 
MfeI C'AATT_G

Cuts at:      0    1143    1542 
   Size:       1143     399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1225    1473    1542 
   Size:       1225     248      69

  Fragments arranged by size: 

                1225     248      69
 
MslI CAynn'nnrTG

Cuts at:      0     816     890    1046    1542 
   Size:        816      74     156     496

  Fragments arranged by size: 

                 816     496     156      74
 
MspA1I CmG'CkG

Cuts at:      0     139     328     337     346    1542 
   Size:        139     189       9       9    1196

  Fragments arranged by size: 

                1196     189     139       9       9
 
NheI G'CTAG_C

Cuts at:      0     775    1542 
   Size:        775     767
 
NsiI A_TGCA'T

Cuts at:      0    1303    1542 
   Size:       1303     239
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     752    1542 
   Size:        752     790
 
PpuMI rG'GwC_Cy

Cuts at:      0    1071    1542 
   Size:       1071     471
 
PstI C_TGCA'G

Cuts at:      0     336     351    1542 
   Size:        336      15    1191

  Fragments arranged by size: 

                1191     336      15
 
PvuII CAG'CTG

Cuts at:      0     328     337     346    1542 
   Size:        328       9       9    1196

  Fragments arranged by size: 

                1196     328       9       9
 
SalI G'TCGA_C

Cuts at:      0     224    1542 
   Size:        224    1318
 
SanDI GG'GwC_CC

Cuts at:      0    1071    1542 
   Size:       1071     471
 
ScaI AGT'ACT

Cuts at:      0     629    1542 
   Size:        629     913
 
SfcI C'TryA_G

Cuts at:      0     169     332     347     419    1437    1542 
   Size:        169     163      15      72    1018     105

  Fragments arranged by size: 

                1018     169     163     105      72      15
 
SspI AAT'ATT

Cuts at:      0    1457    1542 
   Size:       1457      85
 
StyI C'CwwG_G

Cuts at:      0    1035    1542 
   Size:       1035     507
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1304    1542 
   Size:       1304     238
 
TatI w'GTAC_w

Cuts at:      0     273     627     976    1334    1395    1542 
   Size:        273     354     349     358      61     147

  Fragments arranged by size: 

                 358     354     349     273     147      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     579     611    1542 
   Size:        579      32     931

  Fragments arranged by size: 

                 931     579      32
 
XbaI T'CTAG_A

Cuts at:      0     690    1542 
   Size:        690     852
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI    AlwNI     ApoI     AseI     BanI
     BcgI    BfrBI Bme1580I     BmtI     BpmI    BsaBI    BsaXI    BseRI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrGI   BstAPI   BstEII    BstYI
  BstZ17I   Bsu36I     BtsI     DraI     DrdI  Eco57MI EcoO109I     FalI
    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI     MslI   MspA1I
     NheI     NsiI    PflMI    PpuMI     PstI    PvuII     SalI    SanDI
     ScaI     SfcI     SspI     StyI    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI    BmgBI     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI    BstXI     BtgI    BtgZI     ClaI    CspCI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII     HpaI     KasI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I     XcmI
     XhoI     XmaI     XmnI     ZraI
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