Restriction map for pF1KB5068
 (Linear) (Six-base) MAPSORT of: KIBB5068.seq  Check: 118  from: 1  to: 1641



KIBB5068.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1018    1641 
   Size:       1018     623
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      40     383     971    1641 
   Size:         32       8     343     588     670

  Fragments arranged by size: 

                 670     588     343      32       8
 
AflII C'TTAA_G

Cuts at:      0     652    1378    1641 
   Size:        652     726     263

  Fragments arranged by size: 

                 726     652     263
 
AleI CACnn'nnGTG

Cuts at:      0     856    1641 
   Size:        856     785
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     799    1641 
   Size:        799     842
 
AlwNI CAG_nnn'CTG

Cuts at:      0     312     361    1419    1641 
   Size:        312      49    1058     222

  Fragments arranged by size: 

                1058     312     222      49
 
ApaI G_GGCC'C

Cuts at:      0     153     966     998    1231    1641 
   Size:        153     813      32     233     410

  Fragments arranged by size: 

                 813     410     233     153      32
 
ApoI r'AATT_y

Cuts at:      0     730    1615    1641 
   Size:        730     885      26

  Fragments arranged by size: 

                 885     730      26
 
AvaI C'yCGr_G

Cuts at:      0     146     329    1037    1641 
   Size:        146     183     708     604

  Fragments arranged by size: 

                 708     604     183     146
 
AvrII C'CTAG_G

Cuts at:      0    1402    1641 
   Size:       1402     239
 
BanI G'GyrC_C

Cuts at:      0     890    1641 
   Size:        890     751
 
BanII G_rGCy'C

Cuts at:      0     153     163     539     966     998    1231    1641 
   Size:        153      10     376     427      32     233     410

  Fragments arranged by size: 

                 427     410     376     233     153      32      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     175     371     731    1400    1535    1641 
   Size:        175     196     360     669     135     106

  Fragments arranged by size: 

                 669     360     196     175     135     106
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1306    1340    1641 
   Size:       1306      34     301

  Fragments arranged by size: 

                1306     301      34
 
Bme1580I G_kGCm'C

Cuts at:      0     153     966     998    1231    1641 
   Size:        153     813      32     233     410

  Fragments arranged by size: 

                 813     410     233     153      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      28     961    1641 
   Size:         28     933     680

  Fragments arranged by size: 

                 933     680      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     870    1396    1641 
   Size:        499     371     526     245

  Fragments arranged by size: 

                 526     499     371     245
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     604     739    1641 
   Size:        523      81     135     902

  Fragments arranged by size: 

                 902     523     135      81
 
BsaWI w'CCGG_w

Cuts at:      0     228    1020    1253    1641 
   Size:        228     792     233     388

  Fragments arranged by size: 

                 792     388     233     228
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     538    1216    1276    1641 
   Size:        538     678      60     365

  Fragments arranged by size: 

                 678     538     365      60
 
BseYI C'CCAG_C

Cuts at:      0     668    1641 
   Size:        668     973
 
BsiEI CG_ry'CG

Cuts at:      0     176    1641 
   Size:        176    1465
 
BsiHKAI G_wGCw'C

Cuts at:      0     330     539     863    1073    1641 
   Size:        330     209     324     210     568

  Fragments arranged by size: 

                 568     330     324     210     209
 
BsmI GAATG_Cn'

Cuts at:      0    1365    1641 
   Size:       1365     276
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     163     330     539     863     966     998    1073 
   Size:        153      10     167     209     324     103      32      75

Cuts at:   1073    1231    1641 
   Size:        158     410

  Fragments arranged by size: 

                 410     324     209     167     158     153     103      75
                  32      10
 
BspEI T'CCGG_A

Cuts at:      0    1020    1253    1641 
   Size:       1020     233     388

  Fragments arranged by size: 

                1020     388     233
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1018    1641 
   Size:       1018     623
 
BsrFI r'CCGG_y

Cuts at:      0     815    1641 
   Size:        815     826
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     204     946    1641 
   Size:        204     742     695

  Fragments arranged by size: 

                 742     695     204
 
BstBI TT'CG_AA

Cuts at:      0      26    1641 
   Size:         26    1615
 
BstEII G'GTnAC_C

Cuts at:      0     645    1641 
   Size:        645     996
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     279     676    1117    1641 
   Size:        117     162     397     441     524

  Fragments arranged by size: 

                 524     441     397     162     117
 
Bsu36I CC'TnA_GG

Cuts at:      0    1097    1641 
   Size:       1097     544
 
BtgI C'CryG_G

Cuts at:      0    1172    1503    1641 
   Size:       1172     331     138

  Fragments arranged by size: 

                1172     331     138
 
BtsI GCAGTG_nn'

Cuts at:      0     251     877    1454    1585    1641 
   Size:        251     626     577     131      56

  Fragments arranged by size: 

                 626     577     251     131      56
 
DraIII CAC_nnn'GTG

Cuts at:      0    1059    1641 
   Size:       1059     582
 
EaeI y'GGCC_r

Cuts at:      0     173     344    1641 
   Size:        173     171    1297

  Fragments arranged by size: 

                1297     173     171
 
EagI C'GGCC_G

Cuts at:      0     173    1641 
   Size:        173    1468
 
EarI CTCTTCn'nnn_

Cuts at:      0     527    1349    1641 
   Size:        527     822     292

  Fragments arranged by size: 

                 822     527     292
 
EcoICRI GAG'CTC

Cuts at:      0     537    1641 
   Size:        537    1104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32      40     383     499     870     971    1396    1641 
   Size:         32       8     343     116     371     101     425     245

  Fragments arranged by size: 

                 425     371     343     245     116     101      32       8
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1551    1641 
   Size:       1551      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0     135     419     962     963     994    1227    1228    1407 
   Size:        135     284     543       1      31     233       1     179

Cuts at:   1407    1641 
   Size:        234

  Fragments arranged by size: 

                 543     284     234     233     179     135      31       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     523    1404    1641 
   Size:        523     881     237

  Fragments arranged by size: 

                 881     523     237
 
FspI TGC'GCA

Cuts at:      0    1591    1641 
   Size:       1591      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     411     443     997    1029    1641 
   Size:        358      32      21      32     554      32     612

  Fragments arranged by size: 

                 612     554     358      32      32      32      21
 
HincII GTy'rAC

Cuts at:      0     795    1641 
   Size:        795     846
 
HindIII A'AGCT_T

Cuts at:      0    1375    1641 
   Size:       1375     266
 
MscI TGG'CCA

Cuts at:      0     346    1641 
   Size:        346    1295
 
MslI CAynn'nnrTG

Cuts at:      0     856    1641 
   Size:        856     785
 
MspA1I CmG'CkG

Cuts at:      0     201     312     358     672    1416    1641 
   Size:        201     111      46     314     744     225

  Fragments arranged by size: 

                 744     314     225     201     111      46
 
NruI TCG'CGA

Cuts at:      0      84    1641 
   Size:         84    1557
 
PfoI T'CCnGG_A

Cuts at:      0      98     163    1641 
   Size:         98      65    1478

  Fragments arranged by size: 

                1478      98      65
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     242     274    1641 
   Size:        242      32    1367

  Fragments arranged by size: 

                1367     242      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     135     419    1407    1641 
   Size:        135     284     988     234

  Fragments arranged by size: 

                 988     284     234     135
 
PspOMI G'GGCC_C

Cuts at:      0     149     962     994    1227    1641 
   Size:        149     813      32     233     414

  Fragments arranged by size: 

                 813     414     233     149      32
 
PstI C_TGCA'G

Cuts at:      0     200    1291    1641 
   Size:        200    1091     350

  Fragments arranged by size: 

                1091     350     200
 
PvuII CAG'CTG

Cuts at:      0     201     312     358     672    1416    1641 
   Size:        201     111      46     314     744     225

  Fragments arranged by size: 

                 744     314     225     201     111      46
 
SacI G_AGCT'C

Cuts at:      0     539    1641 
   Size:        539    1102
 
SanDI GG'GwC_CC

Cuts at:      0     419    1641 
   Size:        419    1222
 
SapI GCTCTTCn'nnn_

Cuts at:      0     527    1349    1641 
   Size:        527     822     292

  Fragments arranged by size: 

                 822     527     292
 
SexAI A'CCwGG_T

Cuts at:      0    1484    1641 
   Size:       1484     157
 
SfcI C'TryA_G

Cuts at:      0     196    1287    1641 
   Size:        196    1091     354

  Fragments arranged by size: 

                1091     354     196
 
SmaI CCC'GGG

Cuts at:      0    1039    1641 
   Size:       1039     602
 
SmlI C'TyrA_G

Cuts at:      0     538     619     652     754    1378    1641 
   Size:        538      81      33     102     624     263

  Fragments arranged by size: 

                 624     538     263     102      81      33
 
StuI AGG'CCT

Cuts at:      0     254    1012    1102    1641 
   Size:        254     758      90     539

  Fragments arranged by size: 

                 758     539     254      90
 
StyI C'CwwG_G

Cuts at:      0     272     915    1402    1641 
   Size:        272     643     487     239

  Fragments arranged by size: 

                 643     487     272     239
 
Tth111I GACn'n_nGTC

Cuts at:      0     370     931    1641 
   Size:        370     561     710

  Fragments arranged by size: 

                 710     561     370
 
XbaI T'CTAG_A

Cuts at:      0     801    1641 
   Size:        801     840
 
XmaI C'CCGG_G

Cuts at:      0    1037    1641 
   Size:       1037     604
 
XmnI GAAnn'nnTTC

Cuts at:      0    1358    1641 
   Size:       1358     283
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AleI     AlfI    AlwNI     ApaI     ApoI
     AvaI    AvrII     BanI    BanII     BbsI     BcgI Bme1580I     BmrI
     BpmI    BpuEI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspMI    BsrFI   BstAPI    BstBI   BstEII    BstXI
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    Hin4I   HincII  HindIII
     MscI     MslI   MspA1I     NruI     PfoI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SacI    SanDI     SapI    SexAI     SfcI     SmaI
     SmlI     StuI     StyI  Tth111I     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AloI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
     BsgI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EciI    EcoRI    EcoRV     FseI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com