Restriction map for pF1KB5048
 (Linear) (Six-base) MAPSORT of: KIBB5048.seq  Check: 5834  from: 1  to: 2172



KIBB5048.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     147     229    1934    2172 
   Size:        147      82    1705     238

  Fragments arranged by size: 

                1705     238     147      82
 
AatII G_ACGT'C

Cuts at:      0     668    2172 
   Size:        668    1504
 
AccI GT'mk_AC

Cuts at:      0     716    2172 
   Size:        716    1456
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     498     715    1156    2172 
   Size:        213     285     217     441    1016

  Fragments arranged by size: 

                1016     441     285     217     213
 
AflIII A'CryG_T

Cuts at:      0     701    2172 
   Size:        701    1471
 
AleI CACnn'nnGTG

Cuts at:      0     263    2172 
   Size:        263    1909
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     695    2172 
   Size:        695    1477
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1670    1702    2032    2064    2172 
   Size:       1670      32     330      32     108

  Fragments arranged by size: 

                1670     330     108      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     801    1077    1258    1353    2172 
   Size:        801     276     181      95     819

  Fragments arranged by size: 

                 819     801     276     181      95
 
ApaI G_GGCC'C

Cuts at:      0    1036    1305    1625    2172 
   Size:       1036     269     320     547

  Fragments arranged by size: 

                1036     547     320     269
 
ApaLI G'TGCA_C

Cuts at:      0    1591    2172 
   Size:       1591     581
 
AvaI C'yCGr_G

Cuts at:      0     904     923    1754    2159    2172 
   Size:        904      19     831     405      13

  Fragments arranged by size: 

                 904     831     405      19      13
 
BamHI G'GATC_C

Cuts at:      0    1678    2172 
   Size:       1678     494
 
BanI G'GyrC_C

Cuts at:      0     835    2172 
   Size:        835    1337
 
BanII G_rGCy'C

Cuts at:      0     958    1036    1252    1305    1525    1625    2172 
   Size:        958      78     216      53     220     100     547

  Fragments arranged by size: 

                 958     547     220     216     100      78      53
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      56     920    1556    2172 
   Size:         56     864     636     616

  Fragments arranged by size: 

                 864     636     616      56
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     574     608    1582    1616    2001    2035    2172 
   Size:        574      34     974      34     385      34     137

  Fragments arranged by size: 

                 974     574     385     137      34      34      34
 
BfrBI ATG'CAT

Cuts at:      0     672    2172 
   Size:        672    1500
 
BglII A'GATC_T

Cuts at:      0    2096    2172 
   Size:       2096      76
 
Bme1580I G_kGCm'C

Cuts at:      0     838    1036    1305    1595    1625    2172 
   Size:        838     198     269     290      30     547

  Fragments arranged by size: 

                 838     547     290     269     198      30
 
BmgBI CAC'GTC

Cuts at:      0     372    2172 
   Size:        372    1800
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     103    1527    2172 
   Size:        103    1424     645

  Fragments arranged by size: 

                1424     645     103
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     438    2079    2148    2172 
   Size:        438    1641      69      24

  Fragments arranged by size: 

                1641     438      69      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     869    1509    1984    2059    2172 
   Size:         85     784     640     475      75     113

  Fragments arranged by size: 

                 784     640     475     113      85      75
 
Bpu10I CC'TnA_GC

Cuts at:      0    1251    1342    1354    2172 
   Size:       1251      91      12     818

  Fragments arranged by size: 

                1251     818      91      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1435    2172 
   Size:       1435     737
 
BsaAI yAC'GTr

Cuts at:      0     704    2172 
   Size:        704    1468
 
BsaHI Gr'CG_yC

Cuts at:      0     665    2172 
   Size:        665    1507
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     940     970    1507    1537    2032    2062    2172 
   Size:        940      30     537      30     495      30     110

  Fragments arranged by size: 

                 940     537     495     110      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1417    2014    2107    2172 
   Size:       1417     597      93      65

  Fragments arranged by size: 

                1417     597      93      65
 
BseYI C'CCAG_C

Cuts at:      0     736     794    1465    2172 
   Size:        736      58     671     707

  Fragments arranged by size: 

                 736     707     671      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1912    2172 
   Size:       1912     260
 
BsiHKAI G_wGCw'C

Cuts at:      0      71    1595    2172 
   Size:         71    1524     577

  Fragments arranged by size: 

                1524     577      71
 
BsmI GAATG_Cn'

Cuts at:      0    1146    2172 
   Size:       1146    1026
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     838     958    1036    1252    1305    1525    1595 
   Size:         71     767     120      78     216      53     220      70

Cuts at:   1595    1625    2172 
   Size:         30     547

  Fragments arranged by size: 

                 767     547     220     216     120      78      71      70
                  53      30
 
BspHI T'CATG_A

Cuts at:      0    1232    2172 
   Size:       1232     940
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     147     229     908    1934    2172 
   Size:        147      82     679    1026     238

  Fragments arranged by size: 

                1026     679     238     147      82
 
BsrBI CCG'CTC

Cuts at:      0    1958    2172 
   Size:       1958     214
 
BsrDI GCAATG_nn'

Cuts at:      0     747    1127    1163    2172 
   Size:        747     380      36    1009

  Fragments arranged by size: 

                1009     747     380      36
 
BsrFI r'CCGG_y

Cuts at:      0    1801    2172 
   Size:       1801     371
 
BstEII G'GTnAC_C

Cuts at:      0     381    2172 
   Size:        381    1791
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     607     966    1825    2037    2172 
   Size:        607     359     859     212     135

  Fragments arranged by size: 

                 859     607     359     212     135
 
BstYI r'GATC_y

Cuts at:      0     632    1073    1208    1678    2096    2172 
   Size:        632     441     135     470     418      76

  Fragments arranged by size: 

                 632     470     441     418     135      76
 
Bsu36I CC'TnA_GG

Cuts at:      0     331    2172 
   Size:        331    1841
 
BtgI C'CryG_G

Cuts at:      0     959    2172 
   Size:        959    1213
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     219     504    1920    2172 
   Size:        219     285    1416     252

  Fragments arranged by size: 

                1416     285     252     219
 
BtsI GCAGTG_nn'

Cuts at:      0    1872    2172 
   Size:       1872     300
 
ClaI AT'CG_AT

Cuts at:      0    1388    2172 
   Size:       1388     784
 
DraIII CAC_nnn'GTG

Cuts at:      0    1746    2057    2109    2172 
   Size:       1746     311      52      63

  Fragments arranged by size: 

                1746     311      63      52
 
EaeI y'GGCC_r

Cuts at:      0     324     728     890    1025    1728    1803    1892    2172 
   Size:        324     404     162     135     703      75      89     280

  Fragments arranged by size: 

                 703     404     324     280     162     135      89      75
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1178    2172 
   Size:       1178     994
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1939    2172 
   Size:       1939     233
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     213     498     715     869    1156    1509    1984 
   Size:         85     128     285     217     154     287     353     475

Cuts at:   1984    2059    2172 
   Size:         75     113

  Fragments arranged by size: 

                 475     353     287     285     217     154     128     113
                  85      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     412    1345    2172 
   Size:        412     933     827

  Fragments arranged by size: 

                 933     827     412
 
EcoO109I rG'GnC_Cy

Cuts at:      0     496     897    1032    1033    1301    1302    1350    2127 
   Size:        496     401     135       1     268       1      48     777

Cuts at:   2127    2137    2172 
   Size:         10      35

  Fragments arranged by size: 

                 777     496     401     268     135      48      35      10
                   1       1
 
FseI GG_CCGG'CC

Cuts at:      0    1805    2172 
   Size:       1805     367
 
HaeII r_GCGC'y

Cuts at:      0     879    2172 
   Size:        879    1293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     617     649    1152    1184    1507    1539 
   Size:        259      32     326      32     503      32     323      32

Cuts at:   1539    1639    1671    2172 
   Size:        100      32     501

  Fragments arranged by size: 

                 503     501     326     323     259     100      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     120     339     645    1086    2172 
   Size:        120     219     306     441    1086

  Fragments arranged by size: 

                1086     441     306     219     120
 
MscI TGG'CCA

Cuts at:      0     730     892    2172 
   Size:        730     162    1280

  Fragments arranged by size: 

                1280     730     162
 
MslI CAynn'nnrTG

Cuts at:      0     263     605     677     853    1823    2172 
   Size:        263     342      72     176     970     349

  Fragments arranged by size: 

                 970     349     342     263     176      72
 
MspA1I CmG'CkG

Cuts at:      0     394    2172 
   Size:        394    1778
 
NaeI GCC'GGC

Cuts at:      0    1803    2172 
   Size:       1803     369
 
NgoMIV G'CCGG_C

Cuts at:      0    1801    2172 
   Size:       1801     371
 
NsiI A_TGCA'T

Cuts at:      0     674    2172 
   Size:        674    1498
 
NspI r_CATG'y

Cuts at:      0    1384    2172 
   Size:       1384     788
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     801    1517    2172 
   Size:        801     716     655

  Fragments arranged by size: 

                 801     716     655
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2032    2064    2172 
   Size:       2032      32     108

  Fragments arranged by size: 

                2032     108      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     897    2127    2172 
   Size:        897    1230      45

  Fragments arranged by size: 

                1230     897      45
 
PshAI GACnn'nnGTC

Cuts at:      0    1834    2172 
   Size:       1834     338
 
PspOMI G'GGCC_C

Cuts at:      0    1032    1301    1621    2172 
   Size:       1032     269     320     551

  Fragments arranged by size: 

                1032     551     320     269
 
PstI C_TGCA'G

Cuts at:      0    1041    1916    2172 
   Size:       1041     875     256

  Fragments arranged by size: 

                1041     875     256
 
SfcI C'TryA_G

Cuts at:      0    1037    1912    2172 
   Size:       1037     875     260

  Fragments arranged by size: 

                1037     875     260
 
SmaI CCC'GGG

Cuts at:      0    1756    2172 
   Size:       1756     416
 
StyI C'CwwG_G

Cuts at:      0     893    1325    1511    1617    1628    2172 
   Size:        893     432     186     106      11     544

  Fragments arranged by size: 

                 893     544     432     186     106      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     973    1071    1459    2172 
   Size:        184     789      98     388     713

  Fragments arranged by size: 

                 789     713     388     184      98
 
TatI w'GTAC_w

Cuts at:      0    1187    1808    2172 
   Size:       1187     621     364

  Fragments arranged by size: 

                1187     621     364
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     940     972    1409    1441    2172 
   Size:        940      32     437      32     731

  Fragments arranged by size: 

                 940     731     437      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1260    2172 
   Size:       1260     912
 
XmaI C'CCGG_G

Cuts at:      0    1754    2172 
   Size:       1754     418
 
XmnI GAAnn'nnTTC

Cuts at:      0     917    2172 
   Size:        917    1255
 
ZraI GAC'GTC

Cuts at:      0     666    2172 
   Size:        666    1506
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FseI
    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     NaeI   NgoMIV
     NsiI     NspI    PflMI     PpiI    PpuMI    PshAI   PspOMI     PstI
     SfcI     SmaI     StyI    TaqII     TatI     TstI  Tth111I     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI    BciVI     BclI
     BglI     BlpI     BmtI    BpuEI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FalI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     XbaI
     XcmI     XhoI
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