Restriction map for pF1KB4985
##################################
# NO CUT ENZYMES (  125 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspI        AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FseI        HaeIII      
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     Van91I      XhoI        XhoII       


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# ONE CUT ENZYMES (   66 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
AfaI         1112  GTAC                 GT^AC
AflII         847  CTTAAG               C^TTAAG
AflIII       1581  ACGTGT               A^C[GA][CT]GT
AhdI           66  GACAGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          41  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          882  ATTAAT               AT^TAAT
AsnI          882  ATTAAT               AT^TAAT
AspEI          66  GACAGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         120  GAGCAC               G[AT]GC[AT]^C
AvaI          761  CCCGGG               C^[CT]CG[GA]G
AvaII         130  GGACC                G^G[AT]CC
AviII        1191  TGCGCA               TGC^GCA
BbsI         1066  GAAGAC               GAAGAC(2/6)
BcgI         1037  GCAAACTTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         732  GGATAC               GTATCC(6/5)
BfrI          847  CTTAAG               C^TTAAG
BglI          397  GCCACGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          586  CTGGAG               CTGGAG(16/14)
BpuAI        1066  GAAGAC               GAAGAC(2/6)
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BseRI        1226  GAGGAG               GAGGAG(10/8)
BsiHKAI       120  GAGCAC               G[AT]GC[AT]^C
BsoBI         761  CCCGGG               C^[CT]CG[GA]G
BspHI        1055  TCATGA               T^CATGA
BspMI         952  GCAGGT               ACCTGC(4/8)
BstEII        186  GGTAACC              G^GT[ACGT]ACC
BstF5I        364  CATCC                GGATG(2/0)
BstPI         186  GGTAACC              G^GT[ACGT]ACC
BstXI         134  CCAAAGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        130  GGACC                G^G[ACGT]CC
DraII         129  GGGACCC              [GA]G^G[ACGT]CC[CT]
Eam1105I       66  GACAGCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI           52  GAAGAG               CTCTTC(1/4)
EcoNI        1098  CCTTGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      129  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       186  GGTAACC              G^GT[ACGT]ACC
FokI          364  CATCC                GGATG(9/13)
FspI         1191  TGCGCA               TGC^GCA
HaeII         417  GGCGCT               [GA]GCGC^[CT]
HapII         762  CCGG                 C^CGG
HgaI            4  GCGTC                GACGC(5/10)
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HindIII      1024  AAGCTT               A^AGCTT
HpaI           25  GTTAAC               GTT^AAC
HpaII         762  CCGG                 C^CGG
Ksp632I        52  GAAGAG               CTCTTC(1/4)
MspA1I       1520  CAGCTG               C[AC]G^C[GT]G
MspI          762  CCGG                 C^CGG
PpuMI         129  GGGACCC              [GA]G^G[AT]CC[CT]
PshBI         882  ATTAAT               AT^TAAT
PstI          431  CTGCAG               CTGCA^G
PvuII        1520  CAGCTG               CAG^CTG
RcaI         1055  TCATGA               T^CATGA
RsaI         1112  GTAC                 GT^AC
Sau96I        130  GGACC                G^G[ACGT]CC
SexAI        1379  ACCTGGT              A^CC[AT]GGT
SmaI          761  CCCGGG               CCC^GGG
SmlI          847  CTTAAG               C^T[CT][GA]AG
SphI          939  GCATGC               GCATG^C
SspI          481  AATATT               AAT^ATT
TaqI         1046  TCGA                 T^CGA
TthHB8I      1046  TCGA                 T^CGA
XbaI         1210  TCTAGA               T^CTAGA
XmaCI         761  CCCGGG               C^CCGGG
XmaI          761  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BcnI          761  CCCGG                CC^[GC]GG
              762  CCGGG               
BmyI          120  GAGCAC               G[AGT]GC[ACT]^C
              489  GGGCAC              
BsgI         1373  GTGCAG               GTGCAG(16/14)
             1517  GTGCAG              
BsiYI        1011  CCCAGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1098  CCTTGGTGAGG         
BslI         1011  CCCAGAACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1098  CCTTGGTGAGG         
BsmAI          64  GAGAC                GTCTC(1/5)
              107  GAGAC               
Bsp1286I      120  GAGCAC               G[AGT]GC[ACT]^C
              489  GGGCAC              
BsrDI        1166  CATTGC               GCAATG(2/0)
             1303  GCAATG              
BsrI          724  ACTGG                ACTGG(1/-1)
             1017  ACTGG               
BstAPI        784  GCAAAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              943  GCAGATTTTGC         
CfoI          418  GCGC                 GCG^C
             1192  GCGC                
EcoT14I       125  CCTTGG               C^C[AT][AT]GG
             1098  CCTTGG              
FauI          382  GCGGG                CCCGC(4/6)
              487  GCGGG               
HhaI          418  GCGC                 GCG^C
             1192  GCGC                
HinP1I        418  GCGC                 G^CGC
             1192  GCGC                
MaeII         840  ACGT                 A^CGT
             1581  ACGT                
NciI          761  CCCGG                CC^[GC]GG
              762  CCGGG               
NspI          804  ACATGC               [GA]CATG^[CT]
              939  GCATGC              
SfaNI         172  GATGC                GCATC(5/9)
             1174  GATGC               
SfcI          431  CTGCAG               C^T[GA][CT]AG
              548  CTGTAG              
StyI          125  CCTTGG               C^C[AT][AT]GG
             1098  CCTTGG              
Tsp45I        976  GTCAC                ^GT[GC]AC
             1261  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
BanI        BbvI        BclI        BfaI        BsaJI       
BsmFI       BssKI       BstNI       Cac8I       DdeI        
DpnII       EcoRII      FbaI        Fnu4HI      HincII      
HindII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      ScrFI       TfiI        Tru9I       
TseI        Tsp509I     TspRI       XcmI        XmnI        
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