Restriction map for pF1KB4985
 (Linear) (Six-base) MAPSORT of: KIBB4985.seq  Check: 8436  from: 1  to: 1605



KIBB4985.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     943    1605 
   Size:        943     662
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1544    1605 
   Size:       1544      61
 
AflII C'TTAA_G

Cuts at:      0     847    1605 
   Size:        847     758
 
AflIII A'CryG_T

Cuts at:      0    1581    1605 
   Size:       1581      24
 
AhdI GACnn_n'nnGTC

Cuts at:      0      71    1605 
   Size:         71    1534
 
AlwNI CAG_nnn'CTG

Cuts at:      0      46    1605 
   Size:         46    1559
 
ApoI r'AATT_y

Cuts at:      0     149     460     707    1142    1605 
   Size:        149     311     247     435     463

  Fragments arranged by size: 

                 463     435     311     247     149
 
AseI AT'TA_AT

Cuts at:      0     883    1605 
   Size:        883     722
 
AvaI C'yCGr_G

Cuts at:      0     761    1605 
   Size:        761     844
 
BanI G'GyrC_C

Cuts at:      0      91     199    1129    1447    1569    1605 
   Size:         91     108     930     318     122      36

  Fragments arranged by size: 

                 930     318     122     108      91      36
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1073    1605 
   Size:       1073     532
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1026    1060    1605 
   Size:       1026      34     545

  Fragments arranged by size: 

                1026     545      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     726    1605 
   Size:        726     879
 
BclI T'GATC_A

Cuts at:      0     438    1005    1590    1605 
   Size:        438     567     585      15

  Fragments arranged by size: 

                 585     567     438      15
 
BglI GCCn_nnn'nGGC

Cuts at:      0     403    1605 
   Size:        403    1202
 
Bme1580I G_kGCm'C

Cuts at:      0     493    1605 
   Size:        493    1112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1605 
   Size:        607     998
 
Bpu10I CC'TnA_GC

Cuts at:      0    1393    1605 
   Size:       1393     212
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1605 
   Size:          4    1601
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      98     128    1605 
   Size:         98      30    1477

  Fragments arranged by size: 

                1477      98      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1241    1605 
   Size:       1241     364
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1394    1538    1605 
   Size:       1394     144      67

  Fragments arranged by size: 

                1394     144      67
 
BsiHKAI G_wGCw'C

Cuts at:      0     124    1605 
   Size:        124    1481
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     493    1605 
   Size:        124     369    1112

  Fragments arranged by size: 

                1112     369     124
 
BspHI T'CATG_A

Cuts at:      0    1055    1605 
   Size:       1055     550
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     943    1605 
   Size:        943     662
 
BsrDI GCAATG_nn'

Cuts at:      0    1165    1310    1605 
   Size:       1165     145     295

  Fragments arranged by size: 

                1165     295     145
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     790     949    1605 
   Size:        790     159     656

  Fragments arranged by size: 

                 790     656     159
 
BstEII G'GTnAC_C

Cuts at:      0     186    1605 
   Size:        186    1419
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141    1605 
   Size:        141    1464
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      81    1605 
   Size:         81    1524
 
BtsI GCAGTG_nn'

Cuts at:      0     168    1421    1605 
   Size:        168    1253     184

  Fragments arranged by size: 

                1253     184     168
 
EarI CTCTTCn'nnn_

Cuts at:      0      47    1605 
   Size:         47    1558
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     607    1544    1605 
   Size:        607     937      61

  Fragments arranged by size: 

                 937     607      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1102    1605 
   Size:       1102     503
 
EcoO109I rG'GnC_Cy

Cuts at:      0     130    1605 
   Size:        130    1475
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141    1605 
   Size:        141    1464
 
FspI TGC'GCA

Cuts at:      0    1193    1605 
   Size:       1193     412
 
HaeII r_GCGC'y

Cuts at:      0     421    1605 
   Size:        421    1184
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      57      89    1605 
   Size:         57      32    1516

  Fragments arranged by size: 

                1516      57      32
 
HincII GTy'rAC

Cuts at:      0      27     228     774    1605 
   Size:         27     201     546     831

  Fragments arranged by size: 

                 831     546     201      27
 
HindIII A'AGCT_T

Cuts at:      0    1024    1605 
   Size:       1024     581
 
HpaI GTT'AAC

Cuts at:      0      27    1605 
   Size:         27    1578
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     638    1605 
   Size:        367     271     967

  Fragments arranged by size: 

                 967     367     271
 
MspA1I CmG'CkG

Cuts at:      0    1522    1605 
   Size:       1522      83
 
NspI r_CATG'y

Cuts at:      0     808     943    1605 
   Size:        808     135     662

  Fragments arranged by size: 

                 808     662     135
 
PpuMI rG'GwC_Cy

Cuts at:      0     130    1605 
   Size:        130    1475
 
PsiI TTA'TAA

Cuts at:      0     890    1605 
   Size:        890     715
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     542     574    1605 
   Size:        542      32    1031

  Fragments arranged by size: 

                1031     542      32
 
PstI C_TGCA'G

Cuts at:      0     435    1605 
   Size:        435    1170
 
PvuII CAG'CTG

Cuts at:      0    1522    1605 
   Size:       1522      83
 
SanDI GG'GwC_CC

Cuts at:      0     130    1605 
   Size:        130    1475
 
SexAI A'CCwGG_T

Cuts at:      0    1379    1605 
   Size:       1379     226
 
SfcI C'TryA_G

Cuts at:      0     431     548    1605 
   Size:        431     117    1057

  Fragments arranged by size: 

                1057     431     117
 
SmaI CCC'GGG

Cuts at:      0     763    1605 
   Size:        763     842
 
SmlI C'TyrA_G

Cuts at:      0     847    1605 
   Size:        847     758
 
SphI G_CATG'C

Cuts at:      0     943    1605 
   Size:        943     662
 
SspI AAT'ATT

Cuts at:      0     483    1605 
   Size:        483    1122
 
StyI C'CwwG_G

Cuts at:      0     125    1098    1605 
   Size:        125     973     507

  Fragments arranged by size: 

                 973     507     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1025    1587    1605 
   Size:       1025     562      18

  Fragments arranged by size: 

                1025     562      18
 
XbaI T'CTAG_A

Cuts at:      0    1210    1605 
   Size:       1210     395
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108    1428    1458    1605 
   Size:        108    1320      30     147

  Fragments arranged by size: 

                1320     147     108      30
 
XmaI C'CCGG_G

Cuts at:      0     761    1605 
   Size:        761     844
 
XmnI GAAnn'nnTTC

Cuts at:      0     557     842    1487    1605 
   Size:        557     285     645     118

  Fragments arranged by size: 

                 645     557     285     118
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AhdI    AlwNI     ApoI     AseI
     AvaI     BanI     BbsI     BcgI    BciVI     BclI     BglI Bme1580I
     BpmI   Bpu10I    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI   BstAPI   BstEII    BstXI    BtgZI     BtsI
     EarI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     MmeI   MspA1I     NspI    PpuMI     PsiI
     PsrI     PstI    PvuII    SanDI    SexAI     SfcI     SmaI     SmlI
     SphI     SspI     StyI    TaqII     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoRI    EcoRV     FseI    FspAI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     StuI     SwaI     TatI
     TstI  Tth111I     XhoI     ZraI
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