Restriction map for pF1KB4757
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HpaII       ItaI        KasI        
KpnI        KspI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TseI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AvrII         205  CCTAGG               C^CTAGG
BanI         1271  GGCACC               G^G[CT][GA]CC
BfaI          206  CTAG                 C^TAG
BlnI          205  CCTAGG               C^CTAGG
BsaBI         662  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         205  CCTAGG               C^C[ACGT][ACGT]GG
BsgI          444  CTGCAC               GTGCAG(16/14)
BsmBI         349  CGTCTC               CGTCTC(1/5)
BsmI          823  GCATTC               GAATGC(1/-1)
BspLU11I      550  ACATGT               A^CATGT
BspMI        1342  ACCTGC               ACCTGC(4/8)
BstBI         911  TTCGAA               TT^CGAA
DpnII         668  GATC                 ^GATC
EcoNI         228  CCTTTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       205  CCTAGG               C^C[AT][AT]GG
EcoT22I       176  ATGCAT               ATGCA^T
HindIII       318  AAGCTT               A^AGCTT
MaeI          206  CTAG                 C^TAG
MaeII         348  ACGT                 A^CGT
MamI          662  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          668  GATC                 ^GATC
NdeII         668  GATC                 ^GATC
NsiI          176  ATGCAT               ATGCA^T
NspV          911  TTCGAA               TT^CGAA
SacI          498  GAGCTC               GAGCT^C
SapI          902  GCTCTTC              GCTCTTC(1/4)
Sau3AI        668  GATC                 ^GATC
SfcI          362  CTACAG               C^T[GA][CT]AG
SfuI          911  TTCGAA               TT^CGAA
SmlI          333  CTCAAG               C^T[CT][GA]AG
StyI          205  CCTAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          744  CCGC                 CCGC(-3/-1)
             1500  CCGC                
AflIII        346  ACACGT               A^C[GA][CT]GT
              550  ACATGT              
AspHI         498  GAGCTC               G[AT]GC[AT]^C
             1170  GAGCAC              
BanII         498  GAGCTC               G[GA]GC[CT]^C
             1086  GAGCCC              
BsaI          540  GAGACC               GGTCTC(1/5)
             1212  GAGACC              
BsiHKAI       498  GAGCTC               G[AT]GC[AT]^C
             1170  GAGCAC              
BsiYI         228  CCTTTTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              996  CCAAACAAAGG         
BslI          228  CCTTTTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              996  CCAAACAAAGG         
BsrDI         722  GCAATG               GCAATG(2/0)
             1310  GCAATG              
BssKI          52  CCAGG                ^CC[ACGT]GG
              234  CCAGG               
BstNI          52  CCAGG                CC^[AT]GG
              234  CCAGG               
Cac8I          75  GCCTGC               GC[ACGT]^[ACGT]GC
              320  GCTTGC              
EcoRII         52  CCAGG                ^CC[AT]GG
              234  CCAGG               
MseI         1100  TTAA                 T^TAA
             1331  TTAA                
MvaI           52  CCAGG                CC^[AT]GG
              234  CCAGG               
NlaIV         131  GGTTCC               GG[ACGT]^[ACGT]CC
             1271  GGCACC              
NspI          178  GCATGT               [GA]CATG^[CT]
              550  ACATGT              
PleI          106  GACTC                GAGTC(4/5)
              372  GAGTC               
ScrFI          52  CCAGG                CC^[ACGT]GG
              234  CCAGG               
TfiI          154  GATTC                G^A[AT]TC
              220  GAATC               
Tru9I        1100  TTAA                 T^TAA
             1331  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     BmyI        
BpmI        BsmAI       Bsp1286I    BsrI        BstF5I      
DdeI        EarI        EcoRI       FokI        HinfI       
HphI        Ksp632I     MaeIII      MboII       MnlI        
MslI        NdeI        NlaIII      SfaNI       TaqI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XmnI        
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