Restriction map for pF1KB4596
 (Linear) (Six-base) MAPSORT of: KIBB4596.seq  Check: 9566  from: 1  to: 2751



KIBB4596.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2443    2751 
   Size:       2443     308
 
AccI GT'mk_AC

Cuts at:      0    1235    2751 
   Size:       1235    1516
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     111    2347    2751 
   Size:         61      50    2236     404

  Fragments arranged by size: 

                2236     404      61      50
 
AflIII A'CryG_T

Cuts at:      0    2660    2751 
   Size:       2660      91
 
AleI CACnn'nnGTG

Cuts at:      0     362    2496    2716    2751 
   Size:        362    2134     220      35

  Fragments arranged by size: 

                2134     362     220      35
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1532    1896    2751 
   Size:       1532     364     855

  Fragments arranged by size: 

                1532     855     364
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     899     931    2751 
   Size:        899      32    1820

  Fragments arranged by size: 

                1820     899      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18    2751 
   Size:         18    2733
 
ApaLI G'TGCA_C

Cuts at:      0     355    2751 
   Size:        355    2396
 
ApoI r'AATT_y

Cuts at:      0     272    1936    2751 
   Size:        272    1664     815

  Fragments arranged by size: 

                1664     815     272
 
AvaI C'yCGr_G

Cuts at:      0     155     989    2333    2751 
   Size:        155     834    1344     418

  Fragments arranged by size: 

                1344     834     418     155
 
BamHI G'GATC_C

Cuts at:      0     850    2751 
   Size:        850    1901
 
BanI G'GyrC_C

Cuts at:      0     634     697    1171    1302    1978    2751 
   Size:        634      63     474     131     676     773

  Fragments arranged by size: 

                 773     676     634     474     131      63
 
BanII G_rGCy'C

Cuts at:      0    2009    2751 
   Size:       2009     742
 
BbeI G_GCGC'C

Cuts at:      0    1175    2751 
   Size:       1175    1576
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     254     818    2335    2751 
   Size:        254     564    1517     416

  Fragments arranged by size: 

                1517     564     416     254
 
BbvCI CC'TCA_GC

Cuts at:      0    1495    2751 
   Size:       1495    1256
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2458    2751 
   Size:       2458     293
 
BclI T'GATC_A

Cuts at:      0     381     503     683    1725    1847    2231    2751 
   Size:        381     122     180    1042     122     384     520

  Fragments arranged by size: 

                1042     520     384     381     180     122     122
 
BglII A'GATC_T

Cuts at:      0    1673    2312    2751 
   Size:       1673     639     439

  Fragments arranged by size: 

                1673     639     439
 
BlpI GC'TnA_GC

Cuts at:      0    1143    2751 
   Size:       1143    1608
 
Bme1580I G_kGCm'C

Cuts at:      0     359     637    1207    1708    1981    2751 
   Size:        359     278     570     501     273     770

  Fragments arranged by size: 

                 770     570     501     359     278     273
 
BmgBI CAC'GTC

Cuts at:      0    1950    2751 
   Size:       1950     801
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1177    1718    2751 
   Size:       1177     541    1033

  Fragments arranged by size: 

                1177    1033     541
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1474    2377    2395    2598    2751 
   Size:       1474     903      18     203     153

  Fragments arranged by size: 

                1474     903     203     153      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    1495    2751 
   Size:       1495    1256
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1408    2341    2751 
   Size:       1408     933     410

  Fragments arranged by size: 

                1408     933     410
 
BsaAI yAC'GTr

Cuts at:      0    1853    2751 
   Size:       1853     898
 
BsaBI GATnn'nnATC

Cuts at:      0     164     766    2110    2751 
   Size:        164     602    1344     641

  Fragments arranged by size: 

                1344     641     602     164
 
BsaHI Gr'CG_yC

Cuts at:      0    1172    2751 
   Size:       1172    1579
 
BsaWI w'CCGG_w

Cuts at:      0     818    1547    2751 
   Size:        818     729    1204

  Fragments arranged by size: 

                1204     818     729
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     536     566     750     780     899     929    1720    1750 
   Size:        536      30     184      30     119      30     791      30

Cuts at:   1750    2751 
   Size:       1001

  Fragments arranged by size: 

                1001     791     536     184     119      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1318    1426    2017    2077    2751 
   Size:       1318     108     591      60     674

  Fragments arranged by size: 

                1318     674     591     108      60
 
BseYI C'CCAG_C

Cuts at:      0    1488    2206    2751 
   Size:       1488     718     545

  Fragments arranged by size: 

                1488     718     545
 
BsiEI CG_ry'CG

Cuts at:      0    2717    2751 
   Size:       2717      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     359    2751 
   Size:        359    2392
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     743     788    2314    2751 
   Size:        743      45    1526     437

  Fragments arranged by size: 

                1526     743     437      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     637    1207    1708    1981    2009    2751 
   Size:        359     278     570     501     273      28     742

  Fragments arranged by size: 

                 742     570     501     359     278     273      28
 
BspEI T'CCGG_A

Cuts at:      0     818    1547    2751 
   Size:        818     729    1204

  Fragments arranged by size: 

                1204     818     729
 
BspHI T'CATG_A

Cuts at:      0     116     294    2751 
   Size:        116     178    2457

  Fragments arranged by size: 

                2457     178     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2443    2751 
   Size:       2443     308
 
BsrBI CCG'CTC

Cuts at:      0     533    2751 
   Size:        533    2218
 
BsrDI GCAATG_nn'

Cuts at:      0     239    2052    2751 
   Size:        239    1813     699

  Fragments arranged by size: 

                1813     699     239
 
BsrFI r'CCGG_y

Cuts at:      0    1400    2038    2751 
   Size:       1400     638     713

  Fragments arranged by size: 

                1400     713     638
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2051    2751 
   Size:       2051     700
 
BstBI TT'CG_AA

Cuts at:      0     270    2751 
   Size:        270    2481
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1155    2751 
   Size:       1155    1596
 
BstYI r'GATC_y

Cuts at:      0     249     850    1240    1593    1673    2312    2531    2751 
   Size:        249     601     390     353      80     639     219     220

  Fragments arranged by size: 

                 639     601     390     353     249     220     219      80
            
 
BtgI C'CryG_G

Cuts at:      0    1223    1361    1520    2717    2751 
   Size:       1223     138     159    1197      34

  Fragments arranged by size: 

                1223    1197     159     138      34
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2453    2751 
   Size:       2453     298
 
BtsI GCAGTG_nn'

Cuts at:      0     369    2386    2751 
   Size:        369    2017     365

  Fragments arranged by size: 

                2017     369     365
 
DraI TTT'AAA

Cuts at:      0     525    2751 
   Size:        525    2226
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2555    2751 
   Size:       2555     196
 
EaeI y'GGCC_r

Cuts at:      0     938    1388    2714    2751 
   Size:        938     450    1326      37

  Fragments arranged by size: 

                1326     938     450      37
 
EagI C'GGCC_G

Cuts at:      0    2714    2751 
   Size:       2714      37
 
EarI CTCTTCn'nnn_

Cuts at:      0    1459    1709    2305    2751 
   Size:       1459     250     596     446

  Fragments arranged by size: 

                1459     596     446     250
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     981    2751 
   Size:        981    1770
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     111    1474    2347    2377    2395    2598    2751 
   Size:         61      50    1363     873      30      18     203     153

  Fragments arranged by size: 

                1363     873     203     153      61      50      30      18
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1908    2677    2751 
   Size:       1908     769      74

  Fragments arranged by size: 

                1908     769      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0     948    1777    2129    2751 
   Size:        948     829     352     622

  Fragments arranged by size: 

                 948     829     622     352
 
EcoRI G'AATT_C

Cuts at:      0     272    2751 
   Size:        272    2479
 
EcoRV GAT'ATC

Cuts at:      0     114    2160    2751 
   Size:        114    2046     591

  Fragments arranged by size: 

                2046     591     114
 
HaeII r_GCGC'y

Cuts at:      0    1175    1299    2751 
   Size:       1175     124    1452

  Fragments arranged by size: 

                1452    1175     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     787     819     835     867    1090    1122    1407    1439 
   Size:        787      32      16      32     223      32     285      32

Cuts at:   1439    2297    2329    2751 
   Size:        858      32     422

  Fragments arranged by size: 

                 858     787     422     285     223      32      32      32
                  32      32      16
 
HincII GTy'rAC

Cuts at:      0    1236    1965    2751 
   Size:       1236     729     786

  Fragments arranged by size: 

                1236     786     729
 
HindIII A'AGCT_T

Cuts at:      0    2596    2751 
   Size:       2596     155
 
KasI G'GCGC_C

Cuts at:      0    1171    2751 
   Size:       1171    1580
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    2001    2751 
   Size:        193    1808     750

  Fragments arranged by size: 

                1808     750     193
 
MscI TGG'CCA

Cuts at:      0     940    2751 
   Size:        940    1811
 
MslI CAynn'nnrTG

Cuts at:      0     362     640    1514    1984    2116    2496    2652    2716 
   Size:        362     278     874     470     132     380     156      64

Cuts at:   2716    2751 
   Size:         35

  Fragments arranged by size: 

                 874     470     380     362     278     156     132      64
                  35
 
MspA1I CmG'CkG

Cuts at:      0     873    1346    2496    2690    2751 
   Size:        873     473    1150     194      61

  Fragments arranged by size: 

                1150     873     473     194      61
 
NarI GG'CG_CC

Cuts at:      0    1172    2751 
   Size:       1172    1579
 
NcoI C'CATG_G

Cuts at:      0    1361    2751 
   Size:       1361    1390
 
NspI r_CATG'y

Cuts at:      0     900    1449    2751 
   Size:        900     549    1302

  Fragments arranged by size: 

                1302     900     549
 
PfoI T'CCnGG_A

Cuts at:      0    2479    2751 
   Size:       2479     272
 
PmlI CAC'GTG

Cuts at:      0    1853    2751 
   Size:       1853     898
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     899     931    2751 
   Size:        899      32    1820

  Fragments arranged by size: 

                1820     899      32
 
PvuII CAG'CTG

Cuts at:      0     873    2496    2751 
   Size:        873    1623     255

  Fragments arranged by size: 

                1623     873     255
 
SalI G'TCGA_C

Cuts at:      0    1234    2751 
   Size:       1234    1517
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1709    2751 
   Size:       1709    1042
 
SexAI A'CCwGG_T

Cuts at:      0      50    2751 
   Size:         50    2701
 
SfoI GGC'GCC

Cuts at:      0    1173    2751 
   Size:       1173    1578
 
SmaI CCC'GGG

Cuts at:      0     157    2335    2751 
   Size:        157    2178     416

  Fragments arranged by size: 

                2178     416     157
 
SspI AAT'ATT

Cuts at:      0    2194    2751 
   Size:       2194     557
 
StuI AGG'CCT

Cuts at:      0    1043    2751 
   Size:       1043    1708
 
StyI C'CwwG_G

Cuts at:      0     941    1361    2751 
   Size:        941     420    1390

  Fragments arranged by size: 

                1390     941     420
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1224    1274    1582    2239    2393    2751 
   Size:       1224      50     308     657     154     358

  Fragments arranged by size: 

                1224     657     358     308     154      50
 
TatI w'GTAC_w

Cuts at:      0     899    2751 
   Size:        899    1852
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     307     339     536     568    1720    1752    2131    2163 
   Size:        307      32     197      32    1152      32     379      32

Cuts at:   2163    2751 
   Size:        588

  Fragments arranged by size: 

                1152     588     379     307     197      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0      52    1102    2751 
   Size:         52    1050    1649

  Fragments arranged by size: 

                1649    1050      52
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1788    2751 
   Size:       1788     963
 
XmaI C'CCGG_G

Cuts at:      0     155    2333    2751 
   Size:        155    2178     418

  Fragments arranged by size: 

                2178     418     155
 
XmnI GAAnn'nnTTC

Cuts at:      0    2293    2751 
   Size:       2293     458
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstBI    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I
   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI     PfoI     PmlI     PpiI    PvuII     SalI     SapI
    SexAI     SfoI     SmaI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI     BcgI    BfrBI     BglI
     BmtI     BplI    BpuEI     BsgI    BsiWI     BsmI    BsrGI   BssHII
    BssSI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI   DraIII  EcoICRI
     FalI     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII    SanDI
     SbfI     ScaI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI     XbaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com