Restriction map for pF1KB4571
 (Linear) (Six-base) MAPSORT of: KIBB4571.seq  Check: 3818  from: 1  to: 1845



KIBB4571.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0     264     782    1845 
   Size:        264     518    1063

  Fragments arranged by size: 

                1063     518     264
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     964    1845 
   Size:        385     579     881

  Fragments arranged by size: 

                 881     579     385
 
AflIII A'CryG_T

Cuts at:      0     962    1845 
   Size:        962     883
 
ApoI r'AATT_y

Cuts at:      0      13      77     454     723    1512    1845 
   Size:         13      64     377     269     789     333

  Fragments arranged by size: 

                 789     377     333     269      64      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     709     876    1845 
   Size:        709     167     969

  Fragments arranged by size: 

                 969     709     167
 
BfrBI ATG'CAT

Cuts at:      0    1528    1845 
   Size:       1528     317
 
Bme1580I G_kGCm'C

Cuts at:      0     282    1820    1845 
   Size:        282    1538      25

  Fragments arranged by size: 

                1538     282      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     707    1538    1845 
   Size:        707     831     307

  Fragments arranged by size: 

                 831     707     307
 
Bpu10I CC'TnA_GC

Cuts at:      0     407    1845 
   Size:        407    1438
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     859    1038    1845 
   Size:        859     179     807

  Fragments arranged by size: 

                 859     807     179
 
BsaAI yAC'GTr

Cuts at:      0     963    1845 
   Size:        963     882
 
BsaHI Gr'CG_yC

Cuts at:      0     222    1845 
   Size:        222    1623
 
BsaWI w'CCGG_w

Cuts at:      0    1532    1845 
   Size:       1532     313
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      82     112    1367    1397    1845 
   Size:         82      30    1255      30     448

  Fragments arranged by size: 

                1255     448      82      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     644     722    1845 
   Size:        644      78    1123

  Fragments arranged by size: 

                1123     644      78
 
BseYI C'CCAG_C

Cuts at:      0     101    1845 
   Size:        101    1744
 
BsiEI CG_ry'CG

Cuts at:      0     264    1845 
   Size:        264    1581
 
BsmI GAATG_Cn'

Cuts at:      0    1528    1845 
   Size:       1528     317
 
Bsp1286I G_dGCh'C

Cuts at:      0     282    1820    1845 
   Size:        282    1538      25

  Fragments arranged by size: 

                1538     282      25
 
BspEI T'CCGG_A

Cuts at:      0    1532    1845 
   Size:       1532     313
 
BsrGI T'GTAC_A

Cuts at:      0     965    1845 
   Size:        965     880
 
BssSI C'ACGA_G

Cuts at:      0    1754    1845 
   Size:       1754      91
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1644    1845 
   Size:       1644     201
 
BstYI r'GATC_y

Cuts at:      0     708    1845 
   Size:        708    1137
 
BtgI C'CryG_G

Cuts at:      0    1331    1845 
   Size:       1331     514
 
BtsI GCAGTG_nn'

Cuts at:      0     584    1845 
   Size:        584    1261
 
DraI TTT'AAA

Cuts at:      0    1568    1845 
   Size:       1568     277
 
EaeI y'GGCC_r

Cuts at:      0     995    1110    1253    1450    1845 
   Size:        995     115     143     197     395

  Fragments arranged by size: 

                 995     395     197     143     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     507     682     943    1845 
   Size:        507     175     261     902

  Fragments arranged by size: 

                 902     507     261     175
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     964    1845 
   Size:        385     579     881

  Fragments arranged by size: 

                 881     579     385
 
HaeII r_GCGC'y

Cuts at:      0    1054    1845 
   Size:       1054     791
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      82     114     506     538    1011    1043    1845 
   Size:         82      32     392      32     473      32     802

  Fragments arranged by size: 

                 802     473     392      82      32      32      32
 
HincII GTy'rAC

Cuts at:      0     265    1325    1845 
   Size:        265    1060     520

  Fragments arranged by size: 

                1060     520     265
 
HindIII A'AGCT_T

Cuts at:      0    1555    1845 
   Size:       1555     290
 
MfeI C'AATT_G

Cuts at:      0     827    1845 
   Size:        827    1018
 
MscI TGG'CCA

Cuts at:      0    1255    1452    1845 
   Size:       1255     197     393

  Fragments arranged by size: 

                1255     393     197
 
MslI CAynn'nnrTG

Cuts at:      0    1436    1845 
   Size:       1436     409
 
MspA1I CmG'CkG

Cuts at:      0     357    1845 
   Size:        357    1488
 
NcoI C'CATG_G

Cuts at:      0    1331    1845 
   Size:       1331     514
 
NsiI A_TGCA'T

Cuts at:      0    1530    1845 
   Size:       1530     315
 
NspI r_CATG'y

Cuts at:      0    1038    1845 
   Size:       1038     807
 
PstI C_TGCA'G

Cuts at:      0     921    1845 
   Size:        921     924
 
SalI G'TCGA_C

Cuts at:      0     263    1845 
   Size:        263    1582
 
ScaI AGT'ACT

Cuts at:      0    1804    1845 
   Size:       1804      41
 
SexAI A'CCwGG_T

Cuts at:      0     636    1845 
   Size:        636    1209
 
SfcI C'TryA_G

Cuts at:      0     917    1845 
   Size:        917     928
 
SmlI C'TyrA_G

Cuts at:      0     874    1017    1845 
   Size:        874     143     828

  Fragments arranged by size: 

                 874     828     143
 
StyI C'CwwG_G

Cuts at:      0    1250    1331    1664    1845 
   Size:       1250      81     333     181

  Fragments arranged by size: 

                1250     333     181      81
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1176    1636    1845 
   Size:       1176     460     209

  Fragments arranged by size: 

                1176     460     209
 
TatI w'GTAC_w

Cuts at:      0     965    1802    1845 
   Size:        965     837      43

  Fragments arranged by size: 

                 965     837      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1367    1399    1845 
   Size:       1367      32     446

  Fragments arranged by size: 

                1367     446      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1351    1845 
   Size:       1351     494
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApoI     BbsI    BfrBI Bme1580I     BmrI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI     BsmI Bsp1286I    BspEI    BsrGI    BssSI    BstXI    BstYI
     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI    HaeII    Hin4I
   HincII  HindIII     MfeI     MscI     MslI   MspA1I     NcoI     NsiI
     NspI     PstI     SalI     ScaI    SexAI     SfcI     SmlI     StyI
    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaBI     BsgI  BsiHKAI    BsiWI
    BsmBI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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