Restriction map for pF1KB4447
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
AviII       AvrII       BamHI       BbeI        BcgI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HgaI        Hin1I       HindIII     HinfI       HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tsp509I     XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        766  GGTACC               G^GTACC
AflIII        693  ACACGT               A^C[GA][CT]GT
AhdI          201  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         48  GTGCAC               G^TGCAC
AocI           32  CCTCAGG              CC^T[ACGT]AGG
ApaLI          48  GTGCAC               G^TGCAC
Asp700I       790  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       766  GGTACC               G^GTACC
AspEI         201  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         694  CACGTG               CAC^GTG
BbsI          742  GAAGAC               GAAGAC(2/6)
BbvCI         601  GCTGAGG              CCTCAGC(-5/-2)
BciVI         895  GTATCC               GTATCC(6/5)
BclI          165  TGATCA               T^GATCA
BfaI          188  CTAG                 C^TAG
BpuAI         742  GAAGAC               GAAGAC(2/6)
BsaAI         694  CACGTG               [CT]AC^GT[GA]
BseRI         139  CTCCTC               GAGGAG(10/8)
BsmI         1231  GCATTC               GAATGC(1/-1)
BsrDI         169  CATTGC               GCAATG(2/0)
BsrFI         566  GCCGGT               [GA]^CCGG[CT]
BssSI         466  CTCGTG               CACGAG(-5/-1)
BstAPI          3  GCAGGGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I         32  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        566  GCCGGT               [GA]^CCGG[CT]
DraIII        694  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      201  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          541  CTCTTC               CTCTTC(1/4)
Eco81I         32  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       864  CCATGG               C^C[AT][AT]GG
FauI          558  GCGGG                CCCGC(4/6)
FbaI          165  TGATCA               T^GATCA
HincII        526  GTCAAC               GT[CT]^[GA]AC
HindII        526  GTCAAC               GT[CT]^[GA]AC
HphI         1157  TCACC                GGTGA(8/7)
KpnI          766  GGTACC               GGTAC^C
Ksp632I       541  CTCTTC               CTCTTC(1/4)
MaeI          188  CTAG                 C^TAG
NcoI          864  CCATGG               C^CATGG
PflFI         520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         694  CACGTG               CAC^GTG
PmlI          694  CACGTG               CAC^GTG
PstI          553  CTGCAG               CTGCA^G
PvuII         194  CAGCTG               CAG^CTG
SacI          538  GAGCTC               GAGCT^C
SapI          540  GCTCTTC              GCTCTTC(1/4)
ScaI          776  AGTACT               AGT^ACT
SfaNI         858  GATGC                GCATC(5/9)
SmaI          581  CCCGGG               CCC^GGG
StyI          864  CCATGG               C^C[AT][AT]GG
Tth111I       520  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         581  CCCGGG               C^CCGGG
XmaI          581  CCCGGG               C^CCGGG
XmnI          790  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          936  GATCC                GGATC(4/5)
              654  GGATC               
AlwNI        1003  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1121  CAGGACCTG           
AvaI           18  CTCGGG               C^[CT]CG[GA]G
              581  CCCGGG              
BpmI          219  CTCCAG               CTGGAG(16/14)
              574  CTGGAG              
BsgI          628  CTGCAC               GTGCAG(16/14)
              919  CTGCAC              
BsoBI          18  CTCGGG               C^[CT]CG[GA]G
              581  CCCGGG              
BstYI         654  GGATCT               [GA]^GATC[CT]
              935  AGATCC              
DsaI          275  CCACGG               C^C[GA][CT]GG
              864  CCATGG              
EcoNI          17  CCTCGGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              717  CCTCTGCAAGG         
HaeII         563  AGCGCC               [GA]GCGC^[CT]
              613  AGCGCC              
MaeII         449  ACGT                 A^CGT
              695  ACGT                
MaeIII        965  GTGAC                ^GT[ACGT]AC
             1117  GTGAC               
MflI          654  GGATCT               [GA]^GATC[CT]
              935  AGATCC              
MseI          394  TTAA                 T^TAA
             1037  TTAA                
PflMI         414  CCACATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1061  CCATAAACTGG         
SmlI         1030  CTTGAG               C^T[CT][GA]AG
             1224  CTCAAG              
Tru9I         394  TTAA                 T^TAA
             1037  TTAA                
Tsp45I        965  GTGAC                ^GT[GC]AC
             1117  GTGAC               
Van91I        414  CCACATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1061  CCATAAACTGG         
XhoII         654  GGATCT               [GA]^GATC[CT]
              935  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MboI        
MboII       MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TseI        
TspRI       TthHB8I     
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