Restriction map for pF1KB4447
 (Linear) (Six-base) MAPSORT of: KIBB4447.seq  Check: 5732  from: 1  to: 1281



KIBB4447.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      35     560    1281 
   Size:         35     525     721

  Fragments arranged by size: 

                 721     525      35
 
Acc65I G'GTAC_C

Cuts at:      0     766    1281 
   Size:        766     515
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     236     463     761     809    1038    1281 
   Size:        106     130     227     298      48     229     243

  Fragments arranged by size: 

                 298     243     229     227     130     106      48
 
AflIII A'CryG_T

Cuts at:      0     693    1281 
   Size:        693     588
 
AhdI GACnn_n'nnGTC

Cuts at:      0     206    1281 
   Size:        206    1075
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     553     585    1281 
   Size:        553      32     696

  Fragments arranged by size: 

                 696     553      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1008    1126    1281 
   Size:       1008     118     155

  Fragments arranged by size: 

                1008     155     118
 
ApaLI G'TGCA_C

Cuts at:      0      48    1281 
   Size:         48    1233
 
AvaI C'yCGr_G

Cuts at:      0      18     581    1281 
   Size:         18     563     700

  Fragments arranged by size: 

                 700     563      18
 
BanI G'GyrC_C

Cuts at:      0      80     455     766     783    1281 
   Size:         80     375     311      17     498

  Fragments arranged by size: 

                 498     375     311      80      17
 
BanII G_rGCy'C

Cuts at:      0     542     581    1015    1281 
   Size:        542      39     434     266

  Fragments arranged by size: 

                 542     434     266      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     749    1281 
   Size:        749     532
 
BbvCI CC'TCA_GC

Cuts at:      0     602    1281 
   Size:        602     679
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     906    1281 
   Size:        906     375
 
BclI T'GATC_A

Cuts at:      0     165    1281 
   Size:        165    1116
 
Bme1580I G_kGCm'C

Cuts at:      0      52    1281 
   Size:         52    1229
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1077    1281 
   Size:       1077     204
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1112    1281 
   Size:       1112     169
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     204     595    1281 
   Size:        204     391     686

  Fragments arranged by size: 

                 686     391     204
 
Bpu10I CC'TnA_GC

Cuts at:      0     602    1281 
   Size:        602     679
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    1209    1281 
   Size:       1051     158      72

  Fragments arranged by size: 

                1051     158      72
 
BsaAI yAC'GTr

Cuts at:      0     696    1281 
   Size:        696     585
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583     616     646    1281 
   Size:        553      30      33      30     635

  Fragments arranged by size: 

                 635     553      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130    1281 
   Size:        130    1151
 
BseYI C'CCAG_C

Cuts at:      0     192     196     269    1008    1281 
   Size:        192       4      73     739     273

  Fragments arranged by size: 

                 739     273     192      73       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613     904    1281 
   Size:        613     291     377

  Fragments arranged by size: 

                 613     377     291
 
BsiHKAI G_wGCw'C

Cuts at:      0      52     518     542    1281 
   Size:         52     466      24     739

  Fragments arranged by size: 

                 739     466      52      24
 
BsmI GAATG_Cn'

Cuts at:      0    1231    1281 
   Size:       1231      50
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     518     542     581    1015    1281 
   Size:         52     466      24      39     434     266

  Fragments arranged by size: 

                 466     434     266      52      39      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      35     560     635    1134    1281 
   Size:         35     525      75     499     147

  Fragments arranged by size: 

                 525     499     147      75      35
 
BsrDI GCAATG_nn'

Cuts at:      0     168    1281 
   Size:        168    1113
 
BsrFI r'CCGG_y

Cuts at:      0     566    1281 
   Size:        566     715
 
BssSI C'ACGA_G

Cuts at:      0     466    1281 
   Size:        466     815
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0       9    1281 
   Size:          9    1272
 
BstYI r'GATC_y

Cuts at:      0     654     935    1281 
   Size:        654     281     346

  Fragments arranged by size: 

                 654     346     281
 
Bsu36I CC'TnA_GG

Cuts at:      0      33    1281 
   Size:         33    1248
 
BtgI C'CryG_G

Cuts at:      0     275     864    1281 
   Size:        275     589     417

  Fragments arranged by size: 

                 589     417     275
 
BtsI GCAGTG_nn'

Cuts at:      0      82     371     438     827    1281 
   Size:         82     289      67     389     454

  Fragments arranged by size: 

                 454     389     289      82      67
 
DraIII CAC_nnn'GTG

Cuts at:      0     699    1281 
   Size:        699     582
 
EaeI y'GGCC_r

Cuts at:      0     332     608     956    1205    1281 
   Size:        332     276     348     249      76

  Fragments arranged by size: 

                 348     332     276     249      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     547    1281 
   Size:        547     734
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     116    1281 
   Size:        116    1165
 
EcoICRI GAG'CTC

Cuts at:      0     540    1281 
   Size:        540     741
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     204     236     463     595     761     809    1038 
   Size:        106      98      32     227     132     166      48     229

Cuts at:   1038    1281 
   Size:        243

  Fragments arranged by size: 

                 243     229     227     166     132     106      98      48
                  32
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      21     721    1281 
   Size:         21     700     560

  Fragments arranged by size: 

                 700     560      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200     247     324    1123    1220    1281 
   Size:        200      47      77     799      97      61

  Fragments arranged by size: 

                 799     200      97      77      61      47
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     408     525    1281 
   Size:        408     117     756

  Fragments arranged by size: 

                 756     408     117
 
HaeII r_GCGC'y

Cuts at:      0     567     617    1281 
   Size:        567      50     664

  Fragments arranged by size: 

                 664     567      50
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     192     224     736     768    1281 
   Size:        192      32     512      32     513

  Fragments arranged by size: 

                 513     512     192      32      32
 
HincII GTy'rAC

Cuts at:      0     528    1281 
   Size:        528     753
 
KpnI G_GTAC'C

Cuts at:      0     770    1281 
   Size:        770     511
 
MscI TGG'CCA

Cuts at:      0     334     610    1207    1281 
   Size:        334     276     597      74

  Fragments arranged by size: 

                 597     334     276      74
 
MspA1I CmG'CkG

Cuts at:      0     196     558    1005    1281 
   Size:        196     362     447     276

  Fragments arranged by size: 

                 447     362     276     196
 
NcoI C'CATG_G

Cuts at:      0     864    1281 
   Size:        864     417
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     420    1067    1281 
   Size:        420     647     214

  Fragments arranged by size: 

                 647     420     214
 
PfoI T'CCnGG_A

Cuts at:      0     572    1281 
   Size:        572     709
 
PmlI CAC'GTG

Cuts at:      0     696    1281 
   Size:        696     585
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     553     585    1281 
   Size:        553      32     696

  Fragments arranged by size: 

                 696     553      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     200     324    1123    1281 
   Size:        200     124     799     158

  Fragments arranged by size: 

                 799     200     158     124
 
PstI C_TGCA'G

Cuts at:      0     557    1281 
   Size:        557     724
 
PvuII CAG'CTG

Cuts at:      0     196    1281 
   Size:        196    1085
 
SacI G_AGCT'C

Cuts at:      0     542    1281 
   Size:        542     739
 
SanDI GG'GwC_CC

Cuts at:      0     200    1281 
   Size:        200    1081
 
SapI GCTCTTCn'nnn_

Cuts at:      0     547    1281 
   Size:        547     734
 
ScaI AGT'ACT

Cuts at:      0     778    1281 
   Size:        778     503
 
SfcI C'TryA_G

Cuts at:      0     174     376     553     882     931    1281 
   Size:        174     202     177     329      49     350

  Fragments arranged by size: 

                 350     329     202     177     174      49
 
SmaI CCC'GGG

Cuts at:      0     583    1281 
   Size:        583     698
 
SmlI C'TyrA_G

Cuts at:      0    1030    1224    1281 
   Size:       1030     194      57

  Fragments arranged by size: 

                1030     194      57
 
StyI C'CwwG_G

Cuts at:      0     864    1281 
   Size:        864     417
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1174    1281 
   Size:       1174     107
 
TatI w'GTAC_w

Cuts at:      0     776    1281 
   Size:        776     505
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     683     715     817     849    1281 
   Size:        683      32     102      32     432

  Fragments arranged by size: 

                 683     432     102      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     523    1281 
   Size:        523     758
 
XmaI C'CCGG_G

Cuts at:      0     581    1281 
   Size:        581     700
 
XmnI GAAnn'nnTTC

Cuts at:      0     794    1281 
   Size:        794     487
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AhdI     AloI    AlwNI    ApaLI
     AvaI     BanI    BanII     BbsI    BbvCI    BciVI     BclI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI
    BssSI   BstAPI    BstYI   Bsu36I     BtgI     BtsI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     KpnI     MscI   MspA1I     NcoI    PflMI     PfoI
     PmlI     PpiI    PpuMI     PstI    PvuII     SacI    SanDI     SapI
     ScaI     SfcI     SmaI     SmlI     StyI    TaqII     TatI     TstI
  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI     AlfI
     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BsaI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     XbaI     XcmI     XhoI
     ZraI
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