Restriction map for pF1KB4391
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        AhdI        AocI        ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BsaBI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraII       DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII         39  TCCGGA               T^CCGGA
AcyI          778  GACGCC               G[GA]^CG[CT]C
AflIII        499  ACATGT               A^C[GA][CT]GT
Alw44I       1381  GTGCAC               G^TGCAC
AlwNI          24  CAGCGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1052  AGCGCT               AGC^GCT
ApaLI        1381  GTGCAC               G^TGCAC
BalI          162  TGGCCA               TGG^CCA
BanII          18  GAGCTC               G[GA]GC[CT]^C
BbsI         1100  GTCTTC               GAAGAC(2/6)
BlpI          854  GCTCAGC              GC^T[ACGT]AGC
BpmI           21  CTCCAG               CTGGAG(16/14)
Bpu1102I      854  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1100  GTCTTC               GAAGAC(2/6)
BsaHI         778  GACGCC               G[GA]^CG[CT]C
BsaWI          39  TCCGGA               [AT]^CCGG[AT]
BseAI          39  TCCGGA               T^CCGGA
BseRI        1483  GAGGAG               GAGGAG(10/8)
BspEI          39  TCCGGA               T^CCGGA
BspHI         506  TCATGA               T^CATGA
BspLU11I      499  ACATGT               A^CATGT
BsrBI         852  CCGCTC               CCGCTC(-3/-3)
BsrDI          33  CATTGC               GCAATG(2/0)
BsrFI          95  GCCGGT               [GA]^CCGG[CT]
BstAPI         86  GCAGCGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         570  GCCCGC               GC[ACGT]^[ACGT]GC
CelII         854  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         95  GCCGGT               [GA]^CCGG[CT]
DraIII        674  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          162  TGGCCA               [CT]^GGCC[GA]
EarI          658  GAAGAG               CTCTTC(1/4)
Eco47III     1052  AGCGCT               AGC^GCT
HaeII        1052  AGCGCT               [GA]GCGC^[CT]
Hin1I         778  GACGCC               G[GA]^CG[CT]C
HincII        964  GTTAAC               GT[CT]^[GA]AC
HindII        964  GTTAAC               GT[CT]^[GA]AC
HpaI          964  GTTAAC               GTT^AAC
HphI         1494  GGTGA                GGTGA(8/7)
Ksp632I       658  GAAGAG               CTCTTC(1/4)
MluNI         162  TGGCCA               TGG^CCA
MroI           39  TCCGGA               T^CCGGA
MscI          162  TGGCCA               TGG^CCA
MslI         1450  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         1386  CCATGG               C^CATGG
NdeI          975  CATATG               CA^TATG
NspI          499  ACATGT               [GA]CATG^[CT]
PvuII         380  CAGCTG               CAG^CTG
RcaI          506  TCATGA               T^CATGA
SacI           18  GAGCTC               GAGCT^C
ScaI         1462  AGTACT               AGT^ACT
SnaBI        1279  TACGTA               TAC^GTA
XbaI          991  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwI         1003  GATCC                GGATC(4/5)
             1546  GGATC               
AspHI          18  GAGCTC               G[AT]GC[AT]^C
             1381  GTGCAC              
BcnI         1344  CCCGG                CC^[GC]GG
             1394  CCGGG               
BmyI           18  GAGCTC               G[AGT]GC[ACT]^C
             1381  GTGCAC              
BsaAI        1279  TACGTA               [CT]AC^GT[GA]
             1522  TACGTG              
BsiHKAI        18  GAGCTC               G[AT]GC[AT]^C
             1381  GTGCAC              
BsmAI        1135  GAGAC                GTCTC(1/5)
             1486  GAGAC               
BsmFI         324  GGGAC                GGGAC(10/14)
              982  GGGAC               
BsmI          510  GAATGC               GAATGC(1/-1)
              947  GCATTC              
Bsp1286I       18  GAGCTC               G[AGT]GC[ACT]^C
             1381  GTGCAC              
BsrI          327  ACTGG                ACTGG(1/-1)
             1078  CCAGT               
BstF5I        516  CATCC                GGATG(2/0)
             1512  CATCC               
CfoI          795  GCGC                 GCG^C
             1053  GCGC                
DpnII        1003  GATC                 ^GATC
             1547  GATC                
DsaI          575  CCGTGG               C^C[GA][CT]GG
             1386  CCATGG              
EcoRI         787  GAATTC               G^AATTC
              873  GAATTC              
FauI          571  CCCGC                CCCGC(4/6)
              851  CCCGC               
FokI          516  CATCC                GGATG(9/13)
             1512  CATCC               
HgaI          778  GACGC                GACGC(5/10)
               26  GCGTC               
HhaI          795  GCGC                 GCG^C
             1053  GCGC                
HinP1I        795  GCGC                 G^CGC
             1053  GCGC                
MaeII        1280  ACGT                 A^CGT
             1523  ACGT                
MboI         1003  GATC                 ^GATC
             1547  GATC                
NciI         1344  CCCGG                CC^[GC]GG
             1394  CCGGG               
NdeII        1003  GATC                 ^GATC
             1547  GATC                
PleI           43  GACTC                GAGTC(4/5)
              718  GAGTC               
PstI           84  CTGCAG               CTGCA^G
              865  CTGCAG              
Sau3AI       1003  GATC                 ^GATC
             1547  GATC                
SfaNI         187  GATGC                GCATC(5/9)
              916  GCATC               
Tsp45I        525  GTCAC                ^GT[GC]AC
              928  GTCAC               
XcmI          317  CCAAGCTGGGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1533  CCAGGCCAAAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BfaI        BsaJI       BsiYI       
BslI        BssKI       BstNI       Cfr13I      DdeI        
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HinfI       HpaII       ItaI        MaeI        MaeIII      
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfcI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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