Restriction map for
pF1KB4384
(Linear) (Six-base) MAPSORT of: KIBB4384.seq Check: 3777 from: 1 to: 2610
KIBB4384.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 602 2610
Size: 602 2008
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1475 1507 1674 2137 2143 2610
Size: 1475 32 167 463 6 467
Fragments arranged by size:
1475 467 463 167 32 6
AflIII A'CryG_T
Cuts at: 0 482 947 2610
Size: 482 465 1663
Fragments arranged by size:
1663 482 465
AhdI GACnn_n'nnGTC
Cuts at: 0 872 2610
Size: 872 1738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 742 774 1654 1686 2610
Size: 742 32 880 32 924
Fragments arranged by size:
924 880 742 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1433 1719 1773 2163 2610
Size: 1433 286 54 390 447
Fragments arranged by size:
1433 447 390 286 54
ApaI G_GGCC'C
Cuts at: 0 1946 2006 2486 2610
Size: 1946 60 480 124
Fragments arranged by size:
1946 480 124 60
ApaLI G'TGCA_C
Cuts at: 0 519 888 2610
Size: 519 369 1722
Fragments arranged by size:
1722 519 369
ApoI r'AATT_y
Cuts at: 0 835 2610
Size: 835 1775
AvaI C'yCGr_G
Cuts at: 0 56 1189 1299 1392 1639 1795 2066 2371
Size: 56 1133 110 93 247 156 271 305
Cuts at: 2371 2479 2610
Size: 108 131
Fragments arranged by size:
1133 305 271 247 156 131 110 108
93 56
BanI G'GyrC_C
Cuts at: 0 71 1587 2610
Size: 71 1516 1023
Fragments arranged by size:
1516 1023 71
BanII G_rGCy'C
Cuts at: 0 145 650 1190 1796 1946 2006 2067 2486
Size: 145 505 540 606 150 60 61 419
Cuts at: 2486 2595 2610
Size: 109 15
Fragments arranged by size:
606 540 505 419 150 145 109 61
60 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1463 1619 1792 2610
Size: 1463 156 173 818
Fragments arranged by size:
1463 818 173 156
BbvCI CC'TCA_GC
Cuts at: 0 1272 2610
Size: 1272 1338
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 2001 2610
Size: 1207 794 609
Fragments arranged by size:
1207 794 609
BlpI GC'TnA_GC
Cuts at: 0 825 1624 2610
Size: 825 799 986
Fragments arranged by size:
986 825 799
Bme1580I G_kGCm'C
Cuts at: 0 523 892 1379 1946 2006 2486 2546 2610
Size: 523 369 487 567 60 480 60 64
Fragments arranged by size:
567 523 487 480 369 64 60 60
BmgBI CAC'GTC
Cuts at: 0 1053 2079 2610
Size: 1053 1026 531
Fragments arranged by size:
1053 1026 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 996 1403 1905 2412 2610
Size: 996 407 502 507 198
Fragments arranged by size:
996 507 502 407 198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 2599 2610
Size: 1287 1312 11
Fragments arranged by size:
1312 1287 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1795 2155 2610
Size: 976 819 360 455
Fragments arranged by size:
976 819 455 360
Bpu10I CC'TnA_GC
Cuts at: 0 733 1272 2164 2610
Size: 733 539 892 446
Fragments arranged by size:
892 733 539 446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 2610
Size: 538 2072
BsaI GGTCTCn'nnnn_
Cuts at: 0 952 2610
Size: 952 1658
BsaAI yAC'GTr
Cuts at: 0 948 2610
Size: 948 1662
BsaHI Gr'CG_yC
Cuts at: 0 1226 2610
Size: 1226 1384
BsaWI w'CCGG_w
Cuts at: 0 920 2610
Size: 920 1690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 145 175 188 218 2610
Size: 145 30 13 30 2392
Fragments arranged by size:
2392 145 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 196 201 436 608 823 1198 1207
Size: 54 142 5 235 172 215 375 9
Cuts at: 1207 1279 1341 1657 2575 2610
Size: 72 62 316 918 35
Fragments arranged by size:
918 375 316 235 215 172 142 72
62 54 35 9 5
BseYI C'CCAG_C
Cuts at: 0 1202 1351 1679 2550 2610
Size: 1202 149 328 871 60
Fragments arranged by size:
1202 871 328 149 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 2610
Size: 567 2043
BsiHKAI G_wGCw'C
Cuts at: 0 145 523 892 1802 2610
Size: 145 378 369 910 808
Fragments arranged by size:
910 808 378 369 145
BsmBI CGTCTCn'nnnn_
Cuts at: 0 82 1217 2610
Size: 82 1135 1393
Fragments arranged by size:
1393 1135 82
Bsp1286I G_dGCh'C
Cuts at: 0 145 523 650 892 1190 1379 1796 1802
Size: 145 378 127 242 298 189 417 6
Cuts at: 1802 1946 2006 2067 2486 2546 2595 2610
Size: 144 60 61 419 60 49 15
Fragments arranged by size:
419 417 378 298 242 189 145 144
127 61 60 60 49 15 6
BspHI T'CATG_A
Cuts at: 0 506 2610
Size: 506 2104
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1001 2381 2510 2610
Size: 1001 1380 129 100
Fragments arranged by size:
1380 1001 129 100
BsrBI CCG'CTC
Cuts at: 0 495 824 1647 2168 2610
Size: 495 329 823 521 442
Fragments arranged by size:
823 521 495 442 329
BsrDI GCAATG_nn'
Cuts at: 0 293 2610
Size: 293 2317
BsrFI r'CCGG_y
Cuts at: 0 74 1802 2471 2610
Size: 74 1728 669 139
Fragments arranged by size:
1728 669 139 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1433 2610
Size: 1433 1177
BstEII G'GTnAC_C
Cuts at: 0 303 525 796 2610
Size: 303 222 271 1814
Fragments arranged by size:
1814 303 271 222
BstYI r'GATC_y
Cuts at: 0 1900 1923 2610
Size: 1900 23 687
Fragments arranged by size:
1900 687 23
Bsu36I CC'TnA_GG
Cuts at: 0 806 2227 2610
Size: 806 1421 383
Fragments arranged by size:
1421 806 383
BtgI C'CryG_G
Cuts at: 0 299 1064 1309 1316 1517 1601 1889 2093
Size: 299 765 245 7 201 84 288 204
Cuts at: 2093 2610
Size: 517
Fragments arranged by size:
765 517 299 288 245 204 201 84
7
BtsI GCAGTG_nn'
Cuts at: 0 579 1134 1683 2610
Size: 579 555 549 927
Fragments arranged by size:
927 579 555 549
DraIII CAC_nnn'GTG
Cuts at: 0 138 1952 2185 2454 2610
Size: 138 1814 233 269 156
Fragments arranged by size:
1814 269 233 156 138
EaeI y'GGCC_r
Cuts at: 0 819 912 1103 1863 2090 2610
Size: 819 93 191 760 227 520
Fragments arranged by size:
819 760 520 227 191 93
EarI CTCTTCn'nnn_
Cuts at: 0 1147 1516 1890 2138 2610
Size: 1147 369 374 248 472
Fragments arranged by size:
1147 472 374 369 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 93 1672 2610
Size: 93 1579 938
Fragments arranged by size:
1579 938 93
EcoICRI GAG'CTC
Cuts at: 0 143 2610
Size: 143 2467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1475 1507 1674 1795 2137 2143 2155
Size: 976 499 32 167 121 342 6 12
Cuts at: 2155 2610
Size: 455
Fragments arranged by size:
976 499 455 342 167 121 32 12
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2599 2610
Size: 2599 11
EcoO109I rG'GnC_Cy
Cuts at: 0 2002 2003 2160 2175 2313 2483 2580 2610
Size: 2002 1 157 15 138 170 97 30
Fragments arranged by size:
2002 170 157 138 97 30 15 1
EcoRI G'AATT_C
Cuts at: 0 835 2610
Size: 835 1775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1176 2610
Size: 1176 1434
HaeII r_GCGC'y
Cuts at: 0 1996 2610
Size: 1996 614
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 145 164 177 196 374 406 858
Size: 132 13 19 13 19 178 32 452
Cuts at: 858 890 1214 1246 2610
Size: 32 324 32 1364
Fragments arranged by size:
1364 452 324 178 132 32 32 32
19 19 13 13
HincII GTy'rAC
Cuts at: 0 549 1020 2610
Size: 549 471 1590
Fragments arranged by size:
1590 549 471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2132 2610
Size: 2132 478
MscI TGG'CCA
Cuts at: 0 914 1105 1865 2610
Size: 914 191 760 745
Fragments arranged by size:
914 760 745 191
MslI CAynn'nnrTG
Cuts at: 0 298 511 670 751 2610
Size: 298 213 159 81 1859
Fragments arranged by size:
1859 298 213 159 81
MspA1I CmG'CkG
Cuts at: 0 7 1206 1387 1433 1773 2133 2343 2610
Size: 7 1199 181 46 340 360 210 267
Fragments arranged by size:
1199 360 340 267 210 181 46 7
NaeI GCC'GGC
Cuts at: 0 76 2473 2610
Size: 76 2397 137
Fragments arranged by size:
2397 137 76
NcoI C'CATG_G
Cuts at: 0 1064 1309 1517 1601 1889 2093 2610
Size: 1064 245 208 84 288 204 517
Fragments arranged by size:
1064 517 288 245 208 204 84
NgoMIV G'CCGG_C
Cuts at: 0 74 2471 2610
Size: 74 2397 139
Fragments arranged by size:
2397 139 74
NspI r_CATG'y
Cuts at: 0 486 2610
Size: 486 2124
PasI CC'CwG_GG
Cuts at: 0 963 2610
Size: 963 1647
PciI A'CATG_T
Cuts at: 0 482 2610
Size: 482 2128
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 747 1101 1719 1952 2185 2610
Size: 258 489 354 618 233 233 425
Fragments arranged by size:
618 489 425 354 258 233 233
PfoI T'CCnGG_A
Cuts at: 0 1751 2156 2610
Size: 1751 405 454
Fragments arranged by size:
1751 454 405
PpuMI rG'GwC_Cy
Cuts at: 0 2160 2175 2580 2610
Size: 2160 15 405 30
Fragments arranged by size:
2160 405 30 15
PspOMI G'GGCC_C
Cuts at: 0 1942 2002 2482 2610
Size: 1942 60 480 128
Fragments arranged by size:
1942 480 128 60
PspXI vC'TCGA_Gb
Cuts at: 0 2066 2610
Size: 2066 544
PstI C_TGCA'G
Cuts at: 0 998 2610
Size: 998 1612
PvuII CAG'CTG
Cuts at: 0 7 1206 1387 1433 1773 2133 2610
Size: 7 1199 181 46 340 360 477
Fragments arranged by size:
1199 477 360 340 181 46 7
SacI G_AGCT'C
Cuts at: 0 145 2610
Size: 145 2465
SanDI GG'GwC_CC
Cuts at: 0 2175 2610
Size: 2175 435
SapI GCTCTTCn'nnn_
Cuts at: 0 1516 2138 2610
Size: 1516 622 472
Fragments arranged by size:
1516 622 472
SexAI A'CCwGG_T
Cuts at: 0 2181 2610
Size: 2181 429
SfcI C'TryA_G
Cuts at: 0 994 1727 2430 2610
Size: 994 733 703 180
Fragments arranged by size:
994 733 703 180
SmaI CCC'GGG
Cuts at: 0 58 1394 2373 2610
Size: 58 1336 979 237
Fragments arranged by size:
1336 979 237 58
SmlI C'TyrA_G
Cuts at: 0 553 2066 2610
Size: 553 1513 544
Fragments arranged by size:
1513 553 544
StuI AGG'CCT
Cuts at: 0 160 2610
Size: 160 2450
StyI C'CwwG_G
Cuts at: 0 281 893 1064 1309 1517 1532 1601 1889
Size: 281 612 171 245 208 15 69 288
Cuts at: 1889 2093 2610
Size: 204 517
Fragments arranged by size:
612 517 288 281 245 208 204 171
69 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 707 1354 2311 2431 2610
Size: 707 647 957 120 179
Fragments arranged by size:
957 707 647 179 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 188 220 1580 1612 2610
Size: 188 32 1360 32 998
Fragments arranged by size:
1360 998 188 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2043 2610
Size: 2043 567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 1236 1348 2313 2610
Size: 171 1065 112 965 297
Fragments arranged by size:
1065 965 297 171 112
XhoI C'TCGA_G
Cuts at: 0 2066 2610
Size: 2066 544
XmaI C'CCGG_G
Cuts at: 0 56 1392 2371 2610
Size: 56 1336 979 239
Fragments arranged by size:
1336 979 239 56
XmnI GAAnn'nnTTC
Cuts at: 0 1510 2610
Size: 1510 1100
Enzymes that do cut:
AccI AcuI AflIII AhdI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI SapI SexAI SfcI
SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BmtI BsaBI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BssSI BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI
ZraI