Restriction map for pF1KB4321
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       AccII       AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        AspEI       
AspI        AviII       BbeI        BbrPI       BbvCI       
BcgI        BfrI        BglI        BlpI        Bpu1102I    
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BsrBI       BsrGI       BssHII      BssSI       BstBI       
BstUI       Bsu36I      CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       FseI        FspI        HpaI        
KasI        KspI        MluI        MspA1I      MvnI        
NarI        NdeI        NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       1238  GGTACC               G^GTACC
AccIII        149  TCCGGA               T^CCGGA
ApaI           13  GGGCCC               GGGCC^C
Asp700I      1126  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1238  GGTACC               G^GTACC
AspHI        1622  GAGCAC               G[AT]GC[AT]^C
AvrII        2166  CCTAGG               C^CTAGG
BalI          161  TGGCCA               TGG^CCA
BamHI        1974  GGATCC               G^GATCC
BciVI        2971  GGATAC               GTATCC(6/5)
BglII        2400  AGATCT               A^GATCT
BlnI         2166  CCTAGG               C^CTAGG
BsaBI        1975  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         149  TCCGGA               [AT]^CCGG[AT]
BseAI         149  TCCGGA               T^CCGGA
BsiHKAI      1622  GAGCAC               G[AT]GC[AT]^C
BsmI         1086  GAATGC               GAATGC(1/-1)
BspEI         149  TCCGGA               T^CCGGA
BspHI        1277  TCATGA               T^CATGA
BspLU11I     2792  ACATGT               A^CATGT
BstAPI       1184  GCAAGAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2524  CCAATCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI         2702  CCATGG               C^C[GA][CT]GG
HaeII         829  AGCGCC               [GA]GCGC^[CT]
KpnI         1238  GGTACC               GGTAC^C
MamI         1975  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          760  CAATTG               C^AATTG
MluNI         161  TGGCCA               TGG^CCA
MroI          149  TCCGGA               T^CCGGA
MscI          161  TGGCCA               TGG^CCA
MunI          760  CAATTG               C^AATTG
NcoI         2702  CCATGG               C^CATGG
PflMI         235  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1277  TCATGA               T^CATGA
SgrAI          31  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI         1347  CTTGAG               C^T[CT][GA]AG
SnaBI        2810  TACGTA               TAC^GTA
SpeI         1111  ACTAGT               A^CTAGT
Van91I        235  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1126  GAATGGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          682  GTATAC               GT^[AC][GT]AC
             2946  GTATAC              
AcyI           29  GACGCC               G[GA]^CG[CT]C
             1774  GGCGTC              
AflIII       1163  ACGTGT               A^C[GA][CT]GT
             2792  ACATGT              
BclI          477  TGATCA               T^GATCA
             2196  TGATCA              
BsaAI        1625  CACGTA               [CT]AC^GT[GA]
             2810  TACGTA              
BsaHI          29  GACGCC               G[GA]^CG[CT]C
             1774  GGCGTC              
BsaI         1534  GAGACC               GGTCTC(1/5)
             2631  GAGACC              
BsgI           98  CTGCAC               GTGCAG(16/14)
              912  GTGCAG              
BspMI        1204  GCAGGT               ACCTGC(4/8)
             2050  GCAGGT              
Bst1107I      682  GTATAC               GTA^TAC
             2946  GTATAC              
BstZ17I       682  GTATAC               GTA^TAC
             2946  GTATAC              
EaeI           83  TGGCCG               [CT]^GGCC[GA]
              161  TGGCCA              
FbaI          477  TGATCA               T^GATCA
             2196  TGATCA              
Hin1I          29  GACGCC               G[GA]^CG[CT]C
             1774  GGCGTC              
HincII        787  GTCAAC               GT[CT]^[GA]AC
             2788  GTCAAC              
HindII        787  GTCAAC               GT[CT]^[GA]AC
             2788  GTCAAC              
HindIII       543  AAGCTT               A^AGCTT
              992  AAGCTT              
NaeI           32  GCCGGC               GCC^GGC
               85  GCCGGC              
NgoMIV         32  GCCGGC               G^CCGGC
               85  GCCGGC              
SmaI           17  CCCGGG               CCC^GGG
             2468  CCCGGG              
XcmI          235  CCAAAAAATGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1846  CCAAGCCTAATATGG     
XmaCI          17  CCCGGG               C^CCGGG
             2468  CCCGGG              
XmaI           17  CCCGGG               C^CCGGG
             2468  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrDI       BsrFI       BsrI        BssKI       BstEII      
BstF5I      BstNI       BstPI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SphI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       
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